Partial purification and characterization of a triglyceride lipase from cod (Gadus morhua)

Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish

“…In addition, the substrate specificities of triacylglycerol lipases in fish suggest that this enzyme activity will hydrolyse wax esters, although at a much slower rate than triacylglycerols. The bile salt-activated lipase found in teleost fish may be more specific for hydrolysing wax esters than pancreatic lipase (Patton et al, 1975), as has been indicated more recently (Gjellesvik, 1991a,b;Gjellesvik et al, 1989Gjellesvik et al, ,1992.…”

“…Studies had indicated that the lipolytic activity in cod intestinal fluid was stimulated by bile salts and subsequently a bile-salt activated lipase from cod pyloric caeca was purified and extensively characterised (Gjellesvik, 1991a,b;Gjellesvik et al, 1989Gjellesvik et al, ,1992. The enzyme was reportedly very similar to human bile salt-dependent lipase with only minor differences in fatty acid specificity (Gjellesvik, 1991b).…”

“…The precise role(s) of esterase activities in lipid digestion is unclear, but the current understanding of both mammalian and fish bile salt-activated lipase suggests that it could be defined both as a lipase and esterase, depending on substrate used. For instance, the bile salt-activated lipase in cod hydrolysed triacylglycerols, 4-nitrophenyl esters and cholesterol esters (Gjellesvik et al, 1989(Gjellesvik et al, ,1992.…”

Purification of Lipase

“…An important conclusion is that lipases from different tissues subjected to identical purification steps are purified to the same extent. Cod (Gadus morhua) lipases have been treated in two investigations [43,44], which have dealt with two lipases from the same parent source but from different tissue. An identical number of purification steps gave the same extent of purification, lending further credence to the assumption that lipases from the same source show similar extents of purification.…”

Μελέτη Της Σύστασης Κτηνοτροφικών Σπερμάτων Ψυχανθών Και Φύτρου Σπέρματος Χαρουπιού Και Της Επίδρασής Τους Στη Φυσιολογία Και Βιοχημεία Της Πέψης Μεσογειακών Ειδών Ιχθύων

“…Η ενεργότητα της BAL εξαρτάται από την παρουσία χολικών αλάτων για την υδρόλυση των ακυλογλυκερολών, των εστέρων χοληστερόλης και των εστέρων λιποδιαλυτών βιταμινών (Perez-Casanova et al, 2004). Ενεργότητα του τύπου αυτού λιπάσης έχει αναφερθεί και για άλλα είδη ιχθύων όπως Pagrus major (Iijima et al, 1998), Melanogrammus aeglefirms (PerezCasanova et al, 2004), στον βακαλάο (Gadus morrhua) (Gjellesvik et al, 1989, Lie & Lambersten, 1985. Ιδιαίτερα υψηλή ενεργότητα λιπάσης έχει βρεθεί στα πυλωρικά στην πέστροφα (Salmo gairdneri) (Tocher & Sargent, 1984 Μελέτες ωστόσο έχουν δείξει ότι η περιεκτικότητα των σπερμάτων ψυχανθών σε πρωτεΐνες και σε άλλα θρεπτικά, καθώς και αντιθρεπτικά συστατικά διαφέρει μεταξύ ποικιλιών ενός είδους.…”