Partial purification and characterization of manganese-oxidizing factors of Pseudomonas fluorescens GB-1

Okazaki, Megumi; Sugita, Tadaaki; Shimizu, Masato; Ohode, Y.; Iwamoto, Kayoko; Jong, Elisabeth W. de Vrind‐de; Vrind, J. P. M. de; Corstjens, Paul L. A. M.

doi:10.1128/aem.63.12.4793-4799.1997

Cited by 113 publications

(83 citation statements)

References 17 publications

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“…An interesting preliminary finding is the involvement of one or both of a set of two putative sensor kinases belonging to a two-component signal transduction system (Stock et al, 2000) in the production and/or transport of the manganese-oxidizing factor. Manganese oxidation in P. putida GB-1 is first observed in the early stationary phase, and the oxygen concentration in the cultures during growth was shown to influence the manganese-oxidizing activity (Okazaki et al, 1997). Possibly, extracellular signals can affect the production and/or transport of the manganese-oxidizing factor.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel Gsp‐related pathway required for secretion of the manganese‐oxidizing factor of Pseudomonas putida strain GB‐1

Vrind

Groot

Brouwers

et al. 2003

Molecular Microbiology

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SummaryThe manganese-oxidizing factor of Pseudomonas putida strain GB-1 is associated with the outer membrane. One of the systems of protein transport across the outer membrane is the general secretory pathway (Gsp). The gsp genes are called xcp in Pseudomonas species. In a previous study, it was shown that mutation of the prepilin peptidase XcpA and of a homologue of the pseudopilin XcpT inhibited transport of the factor. In the present study, we describe the genomic region flanking the xcpT homologue (designated xcmT1 ). We show that xcmT1 is part of a twogene operon that includes an xcpS homologue (designated xcmS ). No other xcp -like genes are present in the regions flanking the xcmT1 / xcmS cluster. We also characterized the site of transposon insertion of another transport mutant of P. putida GB-1. This insertion appeared to be located in a gene (designated xcmX ) possibly encoding another pseudopilin-related protein. This xcmX is clustered with two other xcpT -related genes (designated xcmT2 and xcmT3 ) on one side and homologues of three csg genes (designated csmE , csmF and csmG ) on the other side. The csg genes are involved in production of aggregative fibres in Escherichia coli and Salmonella typhimurium . A search for XcmX homologues revealed that the recently published genome of Ralstonia solanacearum and the unannotated genome of P. putida KT2440 contain comparable gene clusters with xcmX and xcp homologues that are different from the well-described 'regular' xcp / gsp clusters. They do contain xcpR and xcpQ homologues but, for example, homologues of xcpP , Y and Z are lacking. The results suggest a novel Xcp-related system for the transport of manganese-oxidizing enzymes to the cell surface.

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Section: Discussionmentioning

confidence: 99%

Identification of a novel Gsp‐related pathway required for secretion of the manganese‐oxidizing factor of Pseudomonas putida strain GB‐1

Vrind

Groot

Brouwers

et al. 2003

Molecular Microbiology

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“…After 24 h of growth, MnCl 2 (50 mM) was added to each culture. Production of Mn(IV) was monitored using Leukoberbelin Blue, as described elsewhere (Okazaki et al, 1997). Briefly, the reagent (1 ml) was added to aliquots of each bacterial culture (200 ml) after 24 h in media containing manganese.…”

Section: Isolation Of Bacteria and Assay For Manganese-oxidizing Actimentioning

confidence: 99%

Bacterial and archaeal populations associated with freshwater ferromanganous micronodules and sediments

Stein

Duc

Grundl

et al. 2001

Environmental Microbiology

203

133

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Biology is believed to play a large role in the cycling of iron and manganese in many freshwater environments, but specific microbial groups indigenous to these systems have not been well characterized. To investigate the populations of Bacteria and Archaea associated with metal-rich sediments from Green Bay, WI, we extracted nucleic acids and analysed the phylogenetic relationships of cloned 16S rRNA genes. Because nucleic acids have not been routinely extracted from metal-rich samples, we investigated the bias inherent in DNA extraction and gene amplification from pure MnO2 using defined populations of whole cells or naked DNA. From the sediments, we screened for manganese-oxidizing bacteria using indicator media and found three isolates that were capable of manganese oxidation. In the phylogenetic analysis of bacterial 16S rRNA gene clones, we found two groups related to known metal-oxidizing genera, Leptothrix of the beta-Proteobacteria and Hyphomicrobium of the alpha-Proteobacteria, and a Fe(III)-reducing group related to the Magnetospirillum genus of the alpha-Proteobacteria. Groups related to the metal-reducing delta-Proteobacteria constituted 22% of the gene clones. In addition, gene sequences from one group of methanogens and a group of Crenarchaeota, identified in the archaeal gene clone library, were related to those found previously in Lake Michigan sediments.

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“…strain SG1 (Rosson & Nealson ), Leptothrix discophora strain SS‐1 (Adams & Ghiorse ) and Pseudomonas putida strains MnB1 and GB‐1 (Okazaki et al . ; Banh et al . ).…”

Section: Introductionmentioning

confidence: 99%

Mn accumulation in a submerged plant Egeria densa (Hydrocharitaceae) is mediated by epiphytic bacteria

Tsuji

Asayama

Shiraki

et al. 2017

Plant Cell & Environment

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Many aquatic plants act as biosorbents, removing and recovering metals from the environment. To assess the biosorbent activity of Egeria densa, a submerged freshwater macrophyte, plants were collected monthly from a circular drainage area in Lake Biwa basin and the Mn concentrations of the plants were analysed. Mn concentrations in these plants were generally above those of terrestrial hyperaccumulators, and were markedly higher in spring and summer than in autumn. Mn concentrations were much lower in plants incubated in hydroponic medium at various pH levels with and without Mn supplementation than in field-collected plants. The precipitation of Mn oxides on the leaves was determined by variable pressure scanning electron microscopy-energy dispersive X-ray analysis and Leucoberbelin blue staining. Several strains of epiphytic bacteria were isolated from the field-collected E. densa plants, with many of these strains, including those of the genera Acidovorax, Comamonas, Pseudomonas and Rhizobium, found to have Mn-oxidizing activity. High Mn concentrations in E. densa were mediated by the production of biogenic Mn oxide in biofilms on leaf surfaces. These findings provide new insights into plant epidermal bacterial flora that affect metal accumulation in plants and suggest that these aquatic plants may have use in Mn phytomining.

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Partial purification and characterization of manganese-oxidizing factors of Pseudomonas fluorescens GB-1

Cited by 113 publications

References 17 publications

Identification of a novel Gsp‐related pathway required for secretion of the manganese‐oxidizing factor of Pseudomonas putida strain GB‐1

Identification of a novel Gsp‐related pathway required for secretion of the manganese‐oxidizing factor of Pseudomonas putida strain GB‐1

Bacterial and archaeal populations associated with freshwater ferromanganous micronodules and sediments

Mn accumulation in a submerged plant Egeria densa (Hydrocharitaceae) is mediated by epiphytic bacteria

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