Partial purification and biochemical properties of acid and alkaline phosphatases from Myxococcus coralloides D

González, F.; Fárez-Vidal, María Esther; Arias, Jesús de la Fuente; Montoya, E.

doi:10.1111/j.1365-2672.1994.tb04403.x

Cited by 42 publications

(23 citation statements)

References 31 publications

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“…All analyses were conducted in triplicate. ACP and ALP activities were measured by the p-nitrophenyl phosphate (pNPP) method (González et al 1994). A 100 μL serum sample was incubated for 30 min at 37°C on 2.0 ml substrate (pNPP in citrate buffer, pH 5, for ACP and pNPP in glycine-NaOH buffer, pH 9, for ALP).…”

Section: Lysozyme Acp Alp Sod Activities and Mad Concentrations Amentioning

confidence: 99%

The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the Atlantic salmon (Salmo salar)

Liu

Wang³

2015

Marine and Freshwater Behaviour and Physiology

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Inappropriately high stocking density can lead to chronic stress that causes many problems in fish culture. We examined the influence of stocking density on growth performance and a number of key serum parameters of Atlantic salmon (Salmo salar) to investigate stress-induced changes. Salmon were reared at low-density (~7.57-15.71 kg/m 3 , initial to final density), mediumdensity (~15.12-31.11 kg/m 3 ), and high-density (~30.18-61.34 kg/m 3 ) stocking levels for 10 weeks. The results showed that stocking density had significant influence on the growth rate, specific growth rate, and feed conversion ratio (p < 0.05) but not on condition factor and mortality. All examined seven serum parameters showed significant change in the first few weeks. Five parameters increased in all three stocking densities, while the serum superoxide dismutase (SOD) and alkaline phosphatase (ALP) activities decreased. At the end of the experiment, the immunoglobulin M level and SOD activity were lowest in the high-density group (p < 0.05), while the cortisol level, ALP activity, and maleic dialdehyde content were significantly higher than in the other two groups (p < 0.05). The acid phosphatase (ACP) and lysozyme activities were not affected by stocking density. These results indicate that high stocking density could affect the growth of Atlantic salmon and the selected serum parameters, except for lysozyme and ACP, could be used as good indicators for chronic stress caused by stocking densities that are too high.

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Section: Lysozyme Acp Alp Sod Activities and Mad Concentrations Amentioning

confidence: 99%

The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the Atlantic salmon (Salmo salar)

Liu

Wang³

2015

Marine and Freshwater Behaviour and Physiology

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“…A fraction of haemolymph (0.1 ml) was immediately centrifuged at 6009g for 15 min to separate plasma and haemocytes. The haematological variables viz., total protein, plasma protein, serum protein, haemocyte protein (Bradford 1976), total free amino acids (Yemm and Cocking 1955), phenol oxidase (Soderhall 1981), NBT reduction (Song and Hsieh 1994), alkaline phosphatase and acid phosphatase (Gonzalez et al 1994) were estimated.…”

Section: Extraction Of Haemolymph and Estimation Of Immune Parametersmentioning

confidence: 99%

Pharmacological level of copper induces the immune and antioxidant mechanisms of Fenneropenaeus indicus conferring better protection against white spot syndrome virus infection

Subramannian

Philip

2012

Aquacult Int

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Effect of ambient copper on the immune, antioxidant and lipid peroxidation parameters of Fenneropenaeus indicus and its susceptibility to white spot syndrome virus under heavy metal (Cu 2? ) exposure has been studied. Adult shrimps were acclimated to 25% salinity for a period of 7 days, and after 12 h of starvation, shrimps were dosed with 0.075, 0.150, 0.225 and 0.30 ppm Cu 2? by adding appropriate quantities of copper sulphate solution. After 14 days of metal exposure, the shrimps were challenged with white spot syndrome virus through oral administration and the immune and antioxidant parameters were analysed. Analysis of variance showed that there were significant differences (P \ 0.05) in the immune and antioxidant parameters and lipid peroxidation product in different treatment groups of F. indicus compared to untreated group. The immune parameters and antioxidant enzymes have been significantly lowered (P \ 0.05) in the haemolymph of shrimps dosed with higher concentrations of copper sulphate leading to lipid peroxidation and accumulation of malondialdehyde. However, there was less oxidative stress in shrimps exposed to 0.075 ppm Cu 2? . The present study showed that high levels of Cu 2? enhanced the mortality of F. indicus with concomitant reduction in immune and antioxidant parameters. A concentration of 0.075 ppm Cu 2? in the rearing water was found to have beneficial effect in shrimps in terms of immunostimulation and higher survival against WSSV infection. This can be adopted as a pharmacological approach towards shrimp health management in culture systems.

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“…The SOD activity was assayed using nitroblue tetrazolium (NBT) in the presence of riboflavin and the AKP activity using the p-nitrophenyl phosphate method reported by Gonzalez et al [43]. The assays were done using two commercial kits from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) according to the manufacturer's instruction.…”

Section: Sod and Akp Activity Assaysmentioning

confidence: 99%

Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection

Tang

Liu

Wang

et al. 2010

Fish & Shellfish Immunology

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Partial purification and biochemical properties of acid and alkaline phosphatases from Myxococcus coralloides D

Cited by 42 publications

References 31 publications

The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the Atlantic salmon (Salmo salar)

The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the Atlantic salmon (Salmo salar)

Pharmacological level of copper induces the immune and antioxidant mechanisms of Fenneropenaeus indicus conferring better protection against white spot syndrome virus infection

Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection

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