Partial protection against tissue cysts formation in pigs vaccinated with crude rhoptry proteins of Toxoplasma gondii

Garcia, João Luís; Gennari, Solange Maria; Navarro, Italmar Teodorico; Machado, Rosângela Zacarias; Sinhorini, Idércio Luiz; Freire, Roberta Lemos; Marana, Elizabete Regina Marangoni; Tsutsui, Vinícius Suehiro; Contente, Ana Paula Angelucci; Begale, Leonardo Prudêncio

doi:10.1016/j.vetpar.2005.01.006

Cited by 64 publications

(42 citation statements)

References 24 publications

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“…In sharp contrast, sterile immunity against tissue cyst formation and oocyst shedding has been obtained in pigs (Kringel et al 2004, Garcia et al 2005, Jongert et al 2008a) and cats, respectively , Garcia et al 2007). The highest protection against congenital disease, however, was obtained in a rat model (Zenner et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the most promising protection with purified parasite components was not obtained in mice but in cats, where an intranasally administered rhoptry extract, formulated in QuilA, could provide sterile immunity to oocyst shedding (Garcia et al 2005).…”

Section: Inactivated Parasites Crude or Purified Antigens And Recombmentioning

confidence: 99%

“…Each of these has been used in experimental vaccines for toxoplasmosis over the years with varying degrees of success (Khan et al 1991, Debard et al 1996, Petersen et al 1998, Desholme et al 2000, Martin et al 2004, Cong et al 2005, Garcia et al 2005, Mévé-lec et al 2005, Golkar et al 2007, Zhang et al 2007a, b, Cui et al 2008, Jongert et al 2008b, Xue et al 2008a. A common theme emerges that adjuvants do increase the immune response to the antigen administered and usually, but not always, induce a degree of protection to a subsequent challenge.…”

Section: Adjuvantsmentioning

confidence: 99%

See 2 more Smart Citations

Vaccines against Toxoplasma gondii: challenges and opportunities

Jongert

Roberts

Gargano

et al. 2009

Mem. Inst. Oswaldo Cruz

174

136

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Section: Discussionmentioning

confidence: 99%

Section: Inactivated Parasites Crude or Purified Antigens And Recombmentioning

confidence: 99%

Section: Adjuvantsmentioning

confidence: 99%

See 1 more Smart Citation

Vaccines against Toxoplasma gondii: challenges and opportunities

Jongert

Roberts

Gargano

et al. 2009

Mem. Inst. Oswaldo Cruz

174

136

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show abstract

“…Following experimental inoculation of pigs with any infectious stage of T. gondii (oocysts, tissue cysts, or tachyzoites), a range of antibodies develops against the parasitic antigens. The time interval between the inoculation and the first detection of the immunoglobulins or cytokines varies depending on the virulence of the used strain, the infectious stage, and the applied dose [37,[59][60][61][62].…”

Section: Immune Responses and Tissue Distribution Upon Acute And Chromentioning

confidence: 99%

Prevalence, Genetic Diversity, Tissue Distribution, and Risk Factors Contributing to T. gondii Burden in Domestic Pigs

Jennes¹,

Cox²

2017

Toxoplasmosis

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Toxoplasma gondii is an obligatory intracellular parasite of mammals, including humans and domestic animals. The infection with this parasite has severe clinical consequences, as it causes abortion or fetal abnormalities, encephalitis in immunocompromised humans, ocular toxoplasmosis with chorioretinitis, and it may contribute to Alzheimer disease. Therefore, an efficient control of T. gondii by prevention of the transmission to humans is strongly recommended. Pork is considered as an important source of toxoplasmosis, due to the frequent consumption of the raw or undercooked porcine meat products, a high susceptibility of pigs to the infection, and because of the numerous risk factors, contributing to the prevalence of toxoplasmosis in the pig population. The cellular and humoral immune responses, such as IgM, IgG, IFN-gamma, and interleukin-10 or -12 production, associated with the acute and chronic infection in pigs, do not prevent development of the tissue cysts, which persist lifelong within the intermediate host. Therefore, the prevalence of T. gondii in the pig population might be an useful indication of the risk associated with the consumption of the porcine meat.

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“…Optical density (OD) was read at 490 nm in an ELISA microplate reader. Mean absorbance values were estimated and OD value was calculated as previously described (GARCIA et al, 2005). Positive and negative control sera were included on every plate.…”

Section: Measurement Of Antibody Response -Enzyme Linked Immunosorbenmentioning

confidence: 99%

Toxoplasma gondii: humoral and cellular immune response of BALB/c mice immunized via intranasal route with rTgROP2

Igarashi

Zulpo

Cunha

et al. 2010

Rev. Bras. Parasitol. Vet.

Self Cite

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TgROP2 is an intracellular protein associated with rhoptries of Toxoplama gondii and an antigen component of a candidate vaccine for toxoplasmosis. The purpose of the present study was to evaluate the efficacy of rTgROP2 to stimulate humoral and cellular immune responses in BALB/c mice via intranasal injection. TgROP2 partial coding sequence was (196-561) amplified by PCR from genomic T. gondii RH strain DNA and cloned into the pTrcHis expression vector. Escherichia coli Rosetta 2 cells transformed with pTrcHis-TgROP2 showed high levels (~1 mg.mL-1) of recombinant protein after 4 hours of IPTG induction. Recombinant TgROP2 exhibited an apparent Mr equal to 54 kDa. In order to test immunogenicity of the recombinant protein, 10 BALB/c mice received 10 µg of rROP2 protein + 10 µg of Quil-A via intranasal injection. Doses were administered at days 0, 21, and 42. Three animals were euthanized and used to evaluate cell-ular immune response on day 62. Five (50%) and two (20%) out of ten animals produced IgG (DO mean = 0.307; cut-off = 0.240) and IgA (DO mean = 0.133, cut-off = 0.101), respectively, by ELISA on day 62. The proliferation of splenocytes revealed high stimulation index (SI) when co-cultured with 5, 10 and 15 µg.mL-1 of rTgROP2. These results indicate that intranasal immunization with recombinant protein ROP2 plus Quil-A can elicit both cellular and humoral immune responses in BALB/c mice.

show abstract

Partial protection against tissue cysts formation in pigs vaccinated with crude rhoptry proteins of Toxoplasma gondii

Cited by 64 publications

References 24 publications

Vaccines against Toxoplasma gondii: challenges and opportunities

Vaccines against Toxoplasma gondii: challenges and opportunities

Prevalence, Genetic Diversity, Tissue Distribution, and Risk Factors Contributing to T. gondii Burden in Domestic Pigs

Toxoplasma gondii: humoral and cellular immune response of BALB/c mice immunized via intranasal route with rTgROP2

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