Partial nitritation ANAMMOX in submerged attached growth bioreactors with smart aeration at 20 °C

Shannon, James M.; Hauser, Lee W.; Liu, Xikun; Parkin, Gene F.; Mattes, Timothy E.; Just, Craig L.

doi:10.1039/c4em00481g

Cited by 10 publications

(11 citation statements)

References 24 publications

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“…It makes sense that these groups of N-transforming bacteria co-occur where their substrate microniches overlap, and is likely enhanced by mussels periodically aerating the sediment (Chen & Gu, 2017). Intermittent aeration has shown to enrich microbial cultures in AOB and anammox bacteria in engineered partial nitritation-anammox processes (Shannon et al, 2015; Yang et al, 2015), and similar to our findings, enriches the anammox genus Candidatus Brocadia (Shannon et al, 2015). …”

Section: Discussionsupporting

confidence: 89%

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

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Chimenti

Just

2017

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Targeted qPCR and non-targeted amplicon sequencing of 16S rRNA genes within sediment layers identified the anaerobic ammonium oxidation (anammox) niche and characterized microbial community changes attributable to freshwater mussels. Anammox bacteria were normally distributed (Shapiro-Wilk normality test, W-statistic =0.954, p = 0.773) between 1 and 15 cm depth and were increased by a factor of 2.2 (p < 0.001) at 3 cm below the water-sediment interface when mussels were present. Amplicon sequencing of sediment at depths relevant to mussel burrowing (3 and 5 cm) showed that mussel presence reduced observed species richness (p = 0.005), Chao1 diversity (p = 0.005), and Shannon diversity (p < 0.001), with more pronounced decreases at 5 cm depth. A non-metric, multidimensional scaling model showed that intersample microbial species diversity varied as a function of mussel presence, indicating that sediment below mussels harbored distinct microbial communities. Mussel presence corresponded with a 4-fold decrease in a majority of operational taxonomic units (OTUs) classified in the phyla Gemmatimonadetes, Actinobacteria, Acidobacteria, Plantomycetes, Chloroflexi, Firmicutes, Crenarcheota, and Verrucomicrobia. 38 OTUs in the phylum Nitrospirae were differentially abundant (p < 0.001) with mussels, resulting in an overall increase from 25% to 35%. Nitrogen (N)-cycle OTUs significantly impacted by mussels belonged to anammmox genus Candidatus Brocadia, ammonium oxidizing bacteria family Nitrosomonadaceae, ammonium oxidizing archaea genus Candidatus Nitrososphaera, nitrite oxidizing bacteria in genus Nitrospira, and nitrate- and nitrite-dependent anaerobic methane oxidizing organisms in the archaeal family “ANME-2d” and bacterial phylum “NC10”, respectively. Nitrosomonadaceae (0.9-fold (p < 0.001)) increased with mussels, while NC10 (2.1-fold (p < 0.001)), ANME-2d (1.8-fold (p < 0.001)), and Candidatus Nitrososphaera (1.5-fold (p < 0.001)) decreased with mussels. Co-occurrence of 2-fold increases in Candidatus Brocadia and Nitrospira in shallow sediments suggests that mussels may enhance microbial niches at the interface of oxic–anoxic conditions, presumably through biodeposition and burrowing. Furthermore, it is likely that the niches of Candidatus Nitrososphaera and nitrite- and nitrate-dependent anaerobic methane oxidizers were suppressed by mussel biodeposition and sediment aeration, as these phylotypes require low ammonium concentrations and anoxic conditions, respectively. As far as we know, this is the first study to characterize freshwater mussel impacts on microbial diversity and the vertical distribution of N-cycle microorganisms in upper Mississippi river sediment. These findings advance our understanding of ecosystem services provided by mussels and their impact on aquatic biogeochemical N-cycling.

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Section: Discussionsupporting

confidence: 89%

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Black

Chimenti

Just

2017

PeerJ

Self Cite

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show abstract

“…It makes sense that these groups of N-transforming bacteria cooccur where their substrate microniches overlap, and is likely enhanced by mussels periodically aerating the sediment (Chen & Gu 2017). Intermittent aeration has shown to enrich microbial cultures in AOB and anammox bacteria in engineered partial nitritation-anammox processes (Shannon et al 2015;Yang et al 2015), and similar to our findings, enriches the anammox genus Candidatus Brocadia (Shannon et al 2015).…”

Section: N-cycle Microbial Communitysupporting

confidence: 84%

Peer Review #2 of "Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment (v0.2)"

Lücker¹

2017

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Targeted qPCR and non-targeted amplicon sequencing of 16S rRNA genes within sediment layers identified the anaerobic ammonium oxidation (anammox) niche and characterized microbial community changes attributable to freshwater mussels. Anammox bacteria were normally distributed (Shapiro-Wilk normality test, W-statistic=0.954, p=0.773) between 1-15 cm depth and were increased by a factor of 2.2 (p<0.001) at 3 cm below the watersediment interface when mussels were present. Amplicon sequencing of sediment at depths relevant to mussel burrowing (3 and 5 cm) showed that mussel presence reduced observed species richness (p=0.005), Chao1 diversity (p=0.005), and Shannon diversity (p<0.001), with more pronounced decreases at 5 cm depth. A non-metric, multidimensional scaling model showed that intersample microbial species diversity varied as a function of mussel presence, indicating that sediment below mussels harbored distinct microbial communities. Mussel presence corresponded with a 4-fold decrease in a majority of operational taxonomic units (OTUs) classified in the phyla Gemmatimonadetes, Actinobacteria, Acidobacteria, Plantomycetes, Chloroflexi, Firmicutes, Crenarcheota, and Verrucomicrobia. 38 OTUs in the phylum Nitrospirae were differentially abundant (p<0.001) with mussels, resulting in an overall increase from 25% to 35%. Nitrogen (N)cycle OTUs significantly impacted by mussels belonged to anammmox genus Candidatus Brocadia, ammonium oxidizing bacteria family Nitrosomonadaceae, ammonium oxidizing archaea genus Candidatus Nitrososphaera, nitrite oxidizing bacteria in genus Nitrospira, and nitrate-and nitrite-dependent anaerobic methane oxidizing organisms in the archaeal family "ANME-2d" and bacterial phylum "NC10", respectively. Nitrosomonadaceae (0.9fold (p<0.001)) increased with mussels, while NC10 (2.1-fold (p<0.001)), ANME-2d (1.8fold (p<0.001)), and Candidatus Nitrososphaera (1.5-fold (p<0.001)) decreased with mussels. Co-occurrence of 2-fold increases in Candidatus Brocadia and Nitrospira in shallow sediments suggests that mussels may enhance microbial niches at the interface of

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“…shown to enrich microbial cultures in AOB and anammox bacteria in engineered partial nitritation-anammox processes [147,148], and similar to our findings, enriches the anammox genus Candidatus Brocadia [147].…”

Section: N-cycle Microbial Communitysupporting

confidence: 89%

Assessing the impacts of native freshwater mussels on nitrogen cycling microbial communities using metagenomics

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Partial nitritation ANAMMOX in submerged attached growth bioreactors with smart aeration at 20 °C

Cited by 10 publications

References 24 publications

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Peer Review #2 of "Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment (v0.2)"

Assessing the impacts of native freshwater mussels on nitrogen cycling microbial communities using metagenomics

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