Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico

Abstract

The present study reports partial sequences of a group of genes used as exposure and effect biomarkers of organic contaminants and/or heavy metals in Syacium gunteri. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico.

Influence of Stocking Density on Growth Performance, Intestinal Antioxidant Status, and Immune‐Related Gene Expression of Endangered Megalobrama pellegrini Reared in a Flow‐Through System

“…Cytochrome P450 family 1 subfamily A is associated with xenobiotic biotransformation systems and plays a vital role in response to the contamination of aquatic ecosystems (Puch‐Hau et al. 2018). Meanwhile, CYP1A * has also been considered an unspecific biomarker of the stress response in fish, as its messenger RNA expression increased under crowding and hypoxia (Gornati et al 2004; Ni et al 2014).…”