Partial immune responses in Sichuan bream (Sinibrama taeniatus) after starvation

Abstract

BackgroundFood deprivation is a severe stress across multiple fields and it might be a challenge to immune system.MethodsIn the present study, adult male Sinibrama taeniatus were deprived of feed for 7 to 28 days. We explored the effects of starvation on immunity in S. taeniatus through hematological analysis, antioxidant capacity analysis, detection of the content or activity of immune factors in plasma, and transcriptomic analysis.ResultsThe results indicated that biometric indexes significantly decreased in the fish after starvation, the proportion of thrombocyte, neutrophil and monocyte increased and, conversely, the proportion of lymphocyte decreased. The antioxidant indexes (SOD and CAT) and innate immune parameters (LZM, C3) were upregulated in fish suffering from a short period of starvation, while adaptive immune parameter (IgM) conversely declined. The transcriptome analysis revealed the changes of various metabolic regulatory pathways involved in fatty acids and amino acids, as well as the immune responses and antioxidant capacity.ConclusionsTaken together, this research in the present study suggested an induced innate immunity while a partly suppressed adaptive immunity under a short period starvation.

Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

“…Many studies have been clearly elaborated that the starvation or food-deprived often influenced the function of immunity in mammals (Han et al, 2021;Loft et al, 2022;Forslund, 2023;Janssen et al, 2023) and altered their energy metabolism (Browning et al, 2012;Soeters et al, 2012;Dote-Montero et al, 2022). Similarly, the aforementioned phenomena have also been observed in teleosts (Dai et al, 2018;Liao et al, 2021;Wang et al, 2021;Gou et al, 2023;Shi et al, 2023). Moreover, although some studies have shown that starvation alters the expression of the autophagy-related genes in fish (Balmori-Cedeño et al, 2019;Wu et al, 2020;Pan et al, 2022), there are still many autophagy-related gene from teleosts that have not been identified and characterized.…”

“…In particular, starvation stress is considered to be one of the most important intrinsic factors that not only alters the immune function (Tran et al, 2018;Song et al, 2019;Liao et al, 2021), but also changes basic energy metabolism in teleosts (Dai, W. et al, 2018;Dar et al, 2019;Florescu Gune et al, 2019;Martin et al, 2010). For instance, in Sinibrama taeniatus, the innate immune parameters were increased, but the adaptive immune index was declined after short period of starvation (Shi et al, 2023). In addition, starvation changes the plasma glucose level and increased lysozyme levels in Anguilla anguilla (Caruso et al, 2010).…”