Partial Genome Sequence of a Novel Reo-Like Virus Detected in Asian Citrus Psyllid (Diaphorina citri) Populations from Florida Citrus Groves

Britt, Kellee; Stevens, Kristian; Gebben, Samantha; Levy, Amit; Rwahnih, Maher Al; Batuman, Özgur

doi:10.1128/mra.00563-21

Cited by 4 publications

(5 citation statements)

References 7 publications

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“…In our HTS analysis, several insect and plant viruses were also assembled and annotated. As in the previous survey [ 15 , 38 ], the Citrus tristeza virus (CTV) seems to commonly exist inside psyllids in four regions explored in this study, but was absent from the grove in Vero Beach, Indian River County. The phenomena suggest that psyllids may easily uptake CTV via phloem-feeding [ 15 ], indicating that D. citri also acts as a potential vector of CTV [ 40 ].…”

Section: Discussionsupporting

confidence: 70%

“…In Florida, Britt et al [ 15 ] completed a comprehensive surveys of psyllid populations in the major citrus production regions of 21 counties. The results demonstrated that all these five viruses and another novel reo-like virus ( D. citri cimodo-like virus, DcCLV) existed in citrus groves [ 38 ], and that DcACV was the most prevalent virus (>60%) followed by DcPLV and DcFLV. Additionally, massive reads related to Citrus tristeza virus (CTV) by RNA sequencing were also found in all sample-collecting regions.…”

Section: Introductionmentioning

confidence: 99%

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Identifying the Gut Virome of Diaphorina citri from Florida Groves

Lin

Batuman

Levy

2023

Insects

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Asian citrus psyllid (Diaphorina citri) transmits the bacterial pathogen Candidatus Liberibacter asiaticus (CLas), the putative causative agent of citrus Huanglongbing disease (HLB). Insect-specific viruses can act against insects as their natural enemies, and recently, several D. citri-associated viruses were discovered. The insect gut plays an important role as not only a pool for diverse microbes but also as a physical barrier to prevent the spread of pathogens such as CLas. However, there is little evidence of the presence of D. citri-associated viruses in the gut and of the interaction between them and CLas. Here, we dissected psyllid guts collected from five growing regions in Florida, and the gut virome was analyzed by high throughput sequencing. Four insect viruses, including D. citri-associated C virus (DcACV), D. citri densovirus (DcDV), D. citri reovirus (DcRV), and D. citri flavi-like virus (DcFLV), were identified, and their presence in the gut, including an additional D. citri cimodo-like virus (DcCLV), were confirmed with PCR-based assays. Microscopic analysis showed that DcFLV infection leads to morphological abnormalities in the nuclear structure in the infected psyllid gut cells. The complex and diverse composition of microbiota in the psyllid gut suggests a possible interaction and dynamics between CLas and the D. citri-associated viruses. Our study identified various D. citri-associated viruses that localized in the psyllid gut and provided more information that helps to evaluate the potential vectors for manipulating CLas in the psyllid gut.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Lin

Batuman

Levy

2023

Insects

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“…Multiple study articles have provided evidence for the presence of diverse reo-like viruses, such as the Hubei reo-like virus, the French Guiana reovirus, and the Shenzhen reo-like virus [52][53][54]. A recent study documented the discovery of the Diaphorina citri cimodo-like virus within the Asian citrus psyllid insect [55]. Moreover, the cimodo-like virus in brine shrimp has been detected in brine shrimp cyst products from some countries, including Russia, China, Iran, and the United States [56].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Viral Diversity of Artemia Cysts from Saline Lakes in Kazakhstan Using Viral Metagenomics Analysis

Kumar,

Karamendin,

Mazhibayeva

et al. 2023

Fishes

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Artemia (brine shrimp) holds significant value as a live feed for larval fish and crustaceans, owing to their distinctive dietary requirements. However, it is vital to acknowledge that Artemia also carries potential risk as a vector of infection. We conducted a metagenomic analysis to explore the virome present in Artemia cysts collected from inland salt lakes across four distinct regions in Kazakhstan. This study identified the presence of dsDNA phages and RNA virus sequences, with a predominant representation from the Reoviridae, Nodaviridae, Dicistroviridae, Picornaviridae, Astroviridae, Tombusviridae, and Solemoviridae families. In general, this study has significantly enhanced our understanding of the virome of Artemia cysts in the saline lakes of Kazakhstan; however, the interactions between these putative viruses and brine shrimp and other aquatic animals need further research.

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“…Subsequently, trimmed single-end reads were de novo assembled into contiguous consensus sequences (contigs) using SPAdes version 3.14 with default parameters [ 22 ]. To annotate known viruses, de novo assembled contigs ≥200 bases were compared against a July 2020 copy of the GenBank database from the National Center for Biotechnology Information (NCBI) using BLASTn v. 2.10.1 with a word size of 7 and default parameters [ 23 ]. Finally, for more sensitive identification of viruses within the scope of this study, trimmed reads were aligned to the viral division of the GenBank nucleotide database using bowtie version 2.4.2 [ 24 ] and subsequently processed through PathoScope version 2.0.6 [ 25 ] for absolute read counts for each virus.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing

Britt

Gebben

Levy

et al. 2022

Insects

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The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida’s citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Candidatus Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from citrus groves throughout Florida. Following the widespread detection of sequences aligning to the genome of citrus tristeza virus (CTV) across consecutive years in the Florida ACP virome, we continued to detect a pervasive amount of CTV in Florida ACPs during subsequent years. Simultaneously, we also detected mixed infections of CTV strains in pooled ACPs from different Florida regions. Predating the HLB epidemic, CTV has been present in Florida for many years and our results confirm its widespread and diverse persistence in Florida citrus groves through a unique lens, the ACP. CTV presence in the ACP likely results from feeding on CTV-infected citrus trees in Florida citrus groves, which may help to understand an overlapping presence of CTV and HLB, both endemic citrus pathosystems in the state, and their role in future integrated pest management strategies.

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Partial Genome Sequence of a Novel Reo-Like Virus Detected in Asian Citrus Psyllid (Diaphorina citri) Populations from Florida Citrus Groves

Cited by 4 publications

References 7 publications

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Evaluation of the Viral Diversity of Artemia Cysts from Saline Lakes in Kazakhstan Using Viral Metagenomics Analysis

Analysis of Citrus Tristeza Virus Incidences within Asian Citrus Psyllid (Diaphorina citri) Populations in Florida via High-Throughput Sequencing

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