Partial Functional Diversification ofDrosophila melanogasterSeptin GenesSep2andSep5

O’Neill, Ryan S.; Clark, Denise V.

doi:10.1534/g3.116.028886

Cited by 11 publications

(12 citation statements)

References 82 publications

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“…This group included the SEPT6 (septin-6), EPGN (epithelial mitogen), RDX (radixin), WNT5A (Wnt family member 5A), and EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit) genes. Septins belong to the group of cytoskeleton GTP-binding proteins and take part in cytokinesis during cell division [49]. EPGN stimulated epidermal cell proliferation in the in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

Brązert

Kranc

Chermuła

et al. 2019

JCM

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Granulosa cells (GCs) are a population of somatic cells whose role after ovulation is progesterone production. GCs were collected from patients undergoing controlled ovarian stimulation during an in vitro fertilization procedure, and they were maintained for 1, 7, 15, and 30 days of in vitro primary culture before collection for further gene expression analysis. A study of genes involved in the biological processes of interest was carried out using expression microarrays. To validate the obtained results, Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) was performed. The direction of changes in the expression of the selected genes was confirmed in most of the examples. Six ontological groups (“cell cycle arrest”, “cell cycle process”, “mitotic spindle organization”, “mitotic spindle assembly checkpoint”, “mitotic spindle assembly”, and “mitotic spindle checkpoint”) were analyzed in this study. The results of the microarrays obtained by us allowed us to identify two groups of genes whose expressions were the most upregulated (FAM64A, ANLN, TOP2A, CTGF, CEP55, BIRC5, PRC1, DLGAP5, GAS6, and NDRG1) and the most downregulated (EREG, PID1, INHA, RHOU, CXCL8, SEPT6, EPGN, RDX, WNT5A, and EZH2) during the culture. The cellular ultrastructure showed the presence of structures characteristic of mitotic cell division: a centrosome surrounded by a pericentric matrix, a microtubule system, and a mitotic spindle connected to chromosomes. The main goal of the study was to identify the genes involved in mitotic division and to identify the cellular ultrastructure of GCs in a long-term in vitro culture. All of the genes in these groups were subjected to downstream analysis, and their function and relation to the ovarian environment are discussed. The obtained results suggest that long-term in vitro cultivation of GCs may lead to their differentiation toward another cell type, including cells with cancer-like characteristics.

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Section: Discussionmentioning

confidence: 99%

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

Brązert

Kranc

Chermuła

et al. 2019

JCM

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“…In Drosophila mutations in the GTPase domain of dSEPT1, which belongs to the SEPT2 subgroup, prevents stable septin complex formation whereas mutations that attenuate GTPase function of dSEPT7 do not affect it's ability to form a stable complex with wild-type dSEPT1 and dSEPT2 (Akhmetova et al, 2015). Mutant studies of genes encoding Drosophila SEPT6 subunits ( dSEPT2 and dSEPT5 ) suggest that similar to mammalian cells a SEPT6 class subunit is necessary for functional Septin complexes (O'Neill and Clark, 2016). In mammalian cells, reduction of SEPT7 affects the formation of complexes with septin hexamers but allows formation of heterodimeric and heterotetrameric complexes containing SEPT2 and SEPT6 (Sellin et al, 2011).…”

Section: Septin Subgroups and Regulation Of The Orai Channelmentioning

confidence: 99%

Regulation of Store-Operated Ca2+ Entry by Septins

Deb

Hasan

2016

Front. Cell Dev. Biol.

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The mechanism of store-operated Ca2+ entry (SOCE) brings extracellular Ca2+ into cells after depletion of intracellular Ca2+ stores. Regulation of Ca2+ homeostasis by SOCE helps control various intracellular signaling functions in both non-excitable and excitable cells. Whereas essential components of the SOCE pathway are well characterized, molecular mechanisms underlying regulation of this pathway need investigation. A class of proteins recently demonstrated as regulating SOCE is septins. These are filament-forming GTPases that assemble into higher order structures. One of their most studied cellular functions is as a molecular scaffold that creates diffusion barriers in membranes for a variety of cellular processes. Septins regulate SOCE in mammalian non-excitable cells and in Drosophila neurons. However, the molecular mechanism of SOCE-regulation by septins and the contribution of different subgroups of septins to SOCE-regulation remain to be understood. The regulation of SOCE is relevant in multiple cellular contexts as well as in diseases, such as the Severe Combined Immunodeficiency (SCID) syndrome and neurodegenerative syndromes like Alzheimer's, Spino-Cerebellar Ataxias and Parkinson's. Moreover, Drosophila neurons, where loss of SOCE leads to flight deficits, are a possible cellular template for understanding the molecular basis of neuronal deficits associated with loss of either the Inositol-1,4,5-trisphosphate receptor (IP3R1), a key activator of neuronal SOCE or the Endoplasmic reticulum resident Ca2+ sensor STIM1 (Stromal Interaction Molecule) in mouse. This perspective summarizes our current understanding of septins as regulators of SOCE and discusses the implications for mammalian neuronal function.

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“…Pnut is also expressed in germline and somatic cells of Drosophila ovaries (Akhmetova, et. al 2015), and loss of Sep2 results in sterility (O'Neill and Clark, 2013;O'Neill and Clark, 2016). Septin localization and function in border cells has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Septins regulate border cell shape and surface geometry downstream of Rho

Am¹,

Ja²,

Jp³

et al. 2021

Preprint

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Collective cell migration is prevalent throughout development and common in metastatic tumors, yet this process is not fully understood. In this study, we explore the role of septins (Sep) in collective cell migration, using the Drosophila border cell model. We show that Sep2 and Pnut are expressed in migrating border cells and Sep1, 2, 4, and Peanut (Pnut) are required for migration. Pnut stability depends on the expression of Sep1 and Sep2 in epithelial follicle cells and migratory border cells. We show that knockdown of septins prevents normal protrusion and detachment behaviors. High resolution Airyscan imaging reveals Pnut localization in rings at the base of protrusions. While septins function independently of Cdc42, they colocalize dynamically with nonmuscle myosin II. We suggest that septin polymers may stabilize growing protrusions until sufficient myosin is recruited to retract them.

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Partial Functional Diversification ofDrosophila melanogasterSeptin GenesSep2andSep5

Cited by 11 publications

References 82 publications

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation

Regulation of Store-Operated Ca2+ Entry by Septins

Septins regulate border cell shape and surface geometry downstream of Rho

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