Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice

Rancé, Iann; Tian, Weihua; Mathews, Helena; Kochko, Alexandre de; Beachy, Roger N.; Fauquet, Claude

doi:10.1007/bf00232938

Cited by 62 publications

(32 citation statements)

References 17 publications

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“…They found that a desiccation treatment of 12 hours induced significantly more somatic embryos than 6, 24 or 48 hoursdesiccation. For Indica rice, Rance et al (1994) assumed that the desiccation treatment might trigger rapid biochemical changes in the calli and under water stress specific enzymes or polypeptides probably appear in callus culture.…”

Section: Somatic Embryo Formationmentioning

confidence: 99%

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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Tissue culture techniques enable mass propagation of elite cultivars of date palm (Phoenix dactylifera L.). The main limitations for date palm in vitro multiplication are the low rates achieved using solidified medium and the long period needed to produce acclimatized plantlets. This research focuses on the comparison between different culture types and plant growth regulator (PGR) combinations on callus growth and somatic embryo formation of cv. Zaghlool. For callus growth, 200 mg friable embryogenic callus dispensed in Rasotherm and Phytacon flasks containing 200 ml liquid (100 rotations per minute) and in temporary immersion system, RITA ® bioreactor (5 min immersion every 12 h), were compared with cultures grown on 200 ml solidified medium. For somatic embryo formation, 500 mg of friable embryogenic callus grown in Erlenmeyer flasks filled with 50 ml liquid or solid MS medium. The medium was supplemented with 0.1 mg l -1 Naphthaleneacetic acid (NAA), 1.5 g l -1 activated charcoal (AC) with or without 0.05 mg l -1 6-Benzyl amino purine (BAP) compared to PGR-free medium. Results proved that cell suspension cultures produced the highest callus fresh mass as compared to the other systems tested, and the callus fresh mass reached 4 g after 16 weeks. The temporary immersion system did not significantly enhance the fresh mass of callus compared to the solidified medium. For somatic embryo induction, the number of somatic embryos increased in cell suspensions 6-16 fold compared to the solidified medium. Using liquid MS medium enriched with 0.1 mg l -1 NAA and 1.5 g l -1 AC gave rise to the highest number of somatic embryos formed from 500 mg initial callus: 160 embryos. The number of somatic embryos was also affected by the callus source. The calli induced from leaflet segments excised from converted somatic embryos resulted in a lower somatic embryo number than those of shoot tip origin with about 60 somatic embryos per 500 mg callus. The formation of somatic embryos using liquid medium required only 6 weeks, thus considerably reducing the previously reported period of 18 weeks which is required for somatic embryo formation using solidified media.

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Section: Somatic Embryo Formationmentioning

confidence: 99%

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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“…Rance et al 57 reported that 2-4 folds higher regeneration from 3 h desiccated calli than the control in rice genotypes viz. PN1, IR72 and IR64.…”

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confidence: 98%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

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“…Partial desiccation has been reported to accelerate plant organogenesis or embryogenesis and results in high regeneration ability significantly in grape (Gray, 1989), wheat (Cheng et al, 2003), Brassica napus (Kott & Beversdorf, 1990), rice (Rance et al, 1994;Saharan et al, 2004;Tsukahara & Hirosawa, 1992) and cassava (Mathews et al, 1993). The possible mechanism about its promotion on plant regeneration capacity may be that partial desiccation terminates the developmental mode and "switches" the embryo into a germination mode (Attree et al, 1991).…”

Section: Partial Desiccationmentioning

confidence: 99%

“…The possible mechanism about its promotion on plant regeneration capacity may be that partial desiccation terminates the developmental mode and "switches" the embryo into a germination mode (Attree et al, 1991). Partial desiccation not only enhances the plant regeneration efficiency, but also benefits the plant organogenesis or embryogenesis and subsequent differentiated stage, and thus reduces the whole time in plant tissue culture (Rance et al, 1994). In addition to the positive effect on wheat organogenesis or embryogenesis, partial desiccation during co-culture greatly enhances the transformation efficiency through inhibiting the growth of Agrobacterium which will suppress the recovery of wheat tissue, and favoring the transfer DNA (T-DNA) delivery (Cheng et al, 2003).…”

Section: Partial Desiccationmentioning

confidence: 99%

Bamboo Regeneration via Embryogenesis and Organogenesis

Lin¹,

Huang²,

Wei³

2012

Embryogenesis

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Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice

Cited by 62 publications

References 17 publications

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Bamboo Regeneration via Embryogenesis and Organogenesis

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