Partial depletion of natural gut flora by antibiotic aggravates collagen induced arthritis (CIA) in mice

Dorożyńska, Iwona; Majewska‐Szczepanik, Monika; Marcińska, Katarzyna; Szczepanik, Marian

doi:10.1016/j.pharep.2013.09.007

Cited by 63 publications

(50 citation statements)

References 28 publications

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“…Furthermore, the periodontal pathogens Porphyromonas gingivalis and P. nigrescans markedly aggravate the severity of arthritis in mice with collagen-induced arthritis (CIA) 79 . Treatment with enrofloxacin, an antibiotic that targets Gram-negative bacteria, exacerbated arthritis in the CIA model 80 . Modulation of the gut microbiota with antibiotic treatment also influenced the development of arthritis in the K/B×N model 74 .…”

Section: Evidence From Animal Studiesmentioning

confidence: 99%

How the microbiota shapes rheumatic diseases

et al. 2016

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The human gut harbours a tremendously diverse and abundant microbial community that correlates with, and even modulates, many health-related processes. The mucosal interfaces are particularly active sites of microorganism-host interplay. Growing insight into the characteristic composition and functionality of the mucosal microbiota has revealed that the microbiota is involved in mucosal barrier integrity and immune function. This involvement affects proinflammatory and anti-inflammatory processes not only at the epithelial level, but also at remote sites such as the joints. Here, we review the role of the gut microbiota in shaping local and systemic immune responses and how disturbances in the host-microorganism interplay can potentially affect the development and progression of rheumatic diseases. Increasing our understanding of how to promote host-microorganism homeostasis could therefore reveal novel strategies for the prevention or alleviation of rheumatic disease.

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Section: Evidence From Animal Studiesmentioning

confidence: 99%

How the microbiota shapes rheumatic diseases

et al. 2016

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“…Another important factor in induction of arthritis is the animal gut flora, which has been shown to influence the incidence and severity of IA (43, 44). Notably, variation in gut flora exists from SPF facility to SPF facility and among various strains of mice (32, 4548).…”

Section: Resultsmentioning

confidence: 99%

Head-to-Head Comparison of Protocol Modifications for the Generation of Collagen-Induced Arthritis in a Specific-Pathogen Free Facility Using DBA/1 Mice

Thornton

Strait

2016

BioTechniques

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Collagen-induced arthritis (CIA) is a widely used mouse model for studying inflammatory arthritis (IA). However, CIA induction protocols differ between laboratories, and direct comparison between protocol variations has not been reported. To address this issue, DBA/1 mice housed in conventional and specific-pathogen free (SPF) facilities were administered various combinations of two doses of collagen type II (CII) in complete (CFA) or incomplete Freund's adjuvant (IFA); some mice were also injected with lipopolysaccharide (LPS) and/or additional CII at specific intervals. Mice were evaluated for IA over the subsequent 2 months. Depending directly on the combination of CII, CFA, IFA, and LPS used, the incidence of IA ranged between 20%-100%, and severity extended from mild to severe even in an SPF environment. Our results demonstrate for the first time in head-to-head comparisons that specific variations in the use of CII, CFA, IFA, and LPS can induce a range of arthritic disease intensity and severity in an SPF facility. Thus, distinct experimental settings can be designed for robust assessment of factors that either exacerbate or inhibit arthritis pathogenesis. Furthermore, by achieving 100% incidence in an SPF facility, the protocols provide a practical and humane benefit by reducing the number of mice necessary for experimental assessment.

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“…Evidences illustrated that changes in the symbiotic relationship between intestinal microlora and opportunistic bacteria as well as bacterial translocation from the gut to nasal mucosa or urinary tract may additionally enhance the risk of developing RA as well as disease-related comorbid conditions. Partial depletion of natural gut lora by antibiotic in experimental animals aggravates CIA [53].…”

Section: Altered Gut Microbiota and Inlammation In Rheumatoid Arthritismentioning

confidence: 99%

“…The type 1 T helper (Th1) response develops in response to intracellular pathogens, while type 2 T helper (Th2) and type 17 T helper (Th17) cells are predominantly stimulated after identiication of extracellular organisms [53,54].…”

Section: The Innate and Adaptive Immune Responses Of The Lamina Proprmentioning

confidence: 99%

The Gut Microbiota and Inflammation in Rheumatoid Arthritis

Mohammed¹,

Elmakhzangy²

2017

New Developments in the Pathogenesis of Rheumatoid Arthritis

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Abstract"Mutualism" is a well-deined relationship that describes a form of cooperation between two living organisms of diferent species that ends up with a beneicial outcome for each one. Any disruption to such a relationship by an external trigger or a potential intruder puts at risk the well-being of both. In humans, oral and gut microbiota provide a noteworthy model of beneicial mutualism. Multiple recent evidences point to the possible pathologic consequences of a disruption to this ecosystem (altered microbiota proile or dysbiosis) on human well-being. The gut-joint axis found its clear way "Proof of Principle" in the pathogenesis of autoimmune rheumatic diseases including rheumatoid arthritis (RA), seronegative spondyloarthropathies, and Behcet's disease in a number of studies. Current therapeutic trends are directed towards the diverse biologic and immune-pathogenic factors involved in the disease process. Addressing dysbiosis in RA features an atractive future therapeutic target. In this chapter, authors aim to explore the recent evidences regarding the pathogenic role of "gut dysbiosis" in rheumatoid arthritis (RA), highlighting the spectrum of immune-pathogenic events that might contribute to disease evolution and inspecting future directives of research.

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Partial depletion of natural gut flora by antibiotic aggravates collagen induced arthritis (CIA) in mice

Abstract: Presented data suggest that alteration of gut microbiota via use of enrofloxacin may play a role in modulating arthritis symptom severity in this mouse model.

Cited by 63 publications

References 28 publications

How the microbiota shapes rheumatic diseases

How the microbiota shapes rheumatic diseases

Head-to-Head Comparison of Protocol Modifications for the Generation of Collagen-Induced Arthritis in a Specific-Pathogen Free Facility Using DBA/1 Mice

The Gut Microbiota and Inflammation in Rheumatoid Arthritis

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