Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens

Esposito, Susanna; Scala, Alessia; Bianchini, Sonia; Presicce, Maria Lory; Mori, Alessandro; Sciarrabba, Calogero Sathya; Fior, Giulia; Principi, Nicola

doi:10.1016/j.diagmicrobio.2016.06.018

Cited by 16 publications

(25 citation statements)

References 21 publications

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“…Besides, NxTAG RPP has also improved sensitivity for detecting other respiratory viruses over xTAG RVP (1 adenovirus and 2 bocavirus). These findings corroborate another recent report on the partial comparison between the NxTAG Respiratory Pathogen Panel Assay and the Luminex xTAG Respiratory Panel Fast Assay v2 [32]. NxTAG RPP detected 12 more pathogens than FilmArray RP in our study, with 50% (6/12) being enterovirus/rhinovirus.…”

Section: Clinical Samples and Performance Of Nxtag Rppsupporting

confidence: 92%

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates

Chan

et al. 2017

Advances in Virology

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This study evaluated a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Panel using nasopharyngeal aspirate specimens and culture isolates of different swine/avian-origin influenza A subtypes (H2N2, H5N1, H7N9, H5N6, and H9N2). NxTAG RPP gave sensitivity of 95.2%, specificity of 99.6%, PPV of 93.5%, and NPV of 99.7%. NxTAG RPP, xTAG RVP, and FilmArray RP had highly concordant performance among each other for the detection of respiratory pathogens. The mean analytic sensitivity (TCID50/ml) of NxTAG RPP, xTAG RVP, and FilmArray RP for detection of swine/avian-origin influenza A subtype isolates was 0.7, 41.8, and 0.8, respectively. All three multiplex assays correctly typed and genotyped the influenza viruses, except for NxTAG RRP that could not distinguish H3N2 from H3N2v. Further investigation should be performed if H3N2v is suspected to be the cause of disease. Sensitive and specific laboratory diagnosis of all influenza A viruses subtypes is especially essential in certain epidemic regions, such as Southeast Asia. The results of this study should help clinical laboratory professionals to be aware of the different performances of commercially available molecular multiplex RT-PCR assays that are commonly adopted in many clinical diagnostic laboratories.

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Section: Clinical Samples and Performance Of Nxtag Rppsupporting

confidence: 92%

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates

Chan

et al. 2017

Advances in Virology

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“…Multiplex platforms based on molecular methods can be used only in the hospital, as they require specific equipment and laboratory technicians with considerable knowledge, training, and experience (Beckmann and Hirsch, 2016; Esposito et al, 2016a; Biomerieux, 2018; Fast Track Diagnostic, 2018). Moreover, these platforms have a turnaround time that is significantly shorter than that of culture but generally much longer than that of rapid tests as they take some hours to give reliable results.…”

Section: What Is the Efficiency Of Multiplex Platforms In The Identifmentioning

confidence: 99%

“…Moreover, these platforms have a turnaround time that is significantly shorter than that of culture but generally much longer than that of rapid tests as they take some hours to give reliable results. This can be a limitation in the emergency department or in the intensive care unit, where many patients require immediate diagnostic and therapeutic decisions (Beckmann and Hirsch, 2016; Esposito et al, 2016a; Biomerieux, 2018; Fast Track Diagnostic, 2018). Only the most recently developed platforms, such as the BioFire® FilmArray® Respiratory Panel 2, have an acceptable turnaround time of about 1 h, not much longer than a rapid test (Biofire, 2018).…”

Section: What Is the Efficiency Of Multiplex Platforms In The Identifmentioning

confidence: 99%

“…Multiplex assays are significantly more sensitive and specific compared with rapid immunochromatographic tests and immunofluorescence assays; however, as multiplex assays detect both viable and non-viable viruses and bacteria, they can lead to debatable results (Beckmann and Hirsch, 2016; Esposito et al, 2016a; Biomerieux, 2018; Fast Track Diagnostic, 2018). Generally, the presently developed and marketed multiplex assays exhibit comparable performance with regards to sensitivities and specificities, and detection of coinfections.…”

Section: What Is the Efficiency Of Multiplex Platforms In The Identifmentioning

confidence: 99%

“…Generally, the presently developed and marketed multiplex assays exhibit comparable performance with regards to sensitivities and specificities, and detection of coinfections. A previous comparison of the (Luminex, 2018) Nx TAG RPP assay, BioFire Film Array Respiratory Panel (FA-RP) (Biomerieux, 2018), RespiFinder22 (RF22) (Beckmann and Hirsch, 2016), and the (Luminex, 2018) RVP FAST Assay v2 (Esposito et al, 2016a) in terms of the ability to detect common pathogens revealed that the discordance was lower than 10%, although the turnaround time, workflow simplicity and risk of contamination were lower for the (Luminex, 2018) Nx TAG RPP (Chen et al, 2016; Tang et al, 2016). A systematic review and meta-analysis (Huang et al, 2018) of studies on the accuracy of FA-RP, Nanosphere Verigene RV+ test (Hologic, 2018; Luminex, 2018) Gen-Probe Prodesse assays (Hologic, 2018) in the detection of IV A, IV B virus, RSV, hMPV, and AV showed that all of these assays had high diagnostic accuracy, with an area under the receiver operating characteristic curve (AUROC) equal to or >0.98 for all tested viruses.…”

Section: What Is the Efficiency Of Multiplex Platforms In The Identifmentioning

confidence: 99%

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Multiplex Platforms for the Identification of Respiratory Pathogens: Are They Useful in Pediatric Clinical Practice?

Esposito

Mencacci

Cenci

et al. 2019

Front. Cell. Infect. Microbiol.

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Respiratory tract infections (RTIs) are extremely common especially in the first year of life. Knowledge of the etiology of a RTI is essential to facilitate the appropriate management and the implementation of the most effective control measures. This perspective explains why laboratory methods that can identify pathogens in respiratory secretions have been developed over the course of many years. High-complexity multiplex panel assays that can simultaneously detect up to 20 viruses and up to four bacteria within a few hours have been marketed. However, are these platforms actually useful in pediatric clinical practice? In this manuscript, we showed that these platforms appear to be particularly important for epidemiological studies and clinical research. On the contrary, their routine use in pediatric clinical practice remains debatable. They can be used only in the hospital as they require specific equipment and laboratory technicians with considerable knowledge, training, and experience. Moreover, despite more sensitive and specific than other tests routinely used for respiratory pathogen identification, they do not offer significantly advantage for detection of the true etiology of a respiratory disease. Furthermore, knowledge of which virus is the cause of a respiratory disease is not useful from a therapeutic point of view unless influenza virus or respiratory syncytial virus are the infecting agents as effective drugs are available only for these pathogens. On the other hand, multiplex platforms can be justified in the presence of severe clinical manifestations, and in immunocompromised patients for whom specific treatment option can be available, particularly when they can be used simultaneously with platforms that allow identification of antimicrobial resistance to commonly used drugs. It is highly likely that these platforms, particularly those with high sensitivity and specificity and with low turnaround time, will become essential when new drugs effective and safe against most of the respiratory viruses will be available. Further studies on how to differentiate carriers from patients with true disease, as well as studies on the implications of coinfections and identification of antimicrobial resistance, are warranted.

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Simultaneous detection of 9 respiratory pathogens using a newly developed multiplex real-time PCR panel based on an automatic molecular detection and analysis system

Zhao

Jiang

et al. 2022

Diagnostic Microbiology and Infectious Disease

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Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens

Cited by 16 publications

References 21 publications

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates

Multiplex Platforms for the Identification of Respiratory Pathogens: Are They Useful in Pediatric Clinical Practice?

Simultaneous detection of 9 respiratory pathogens using a newly developed multiplex real-time PCR panel based on an automatic molecular detection and analysis system

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