Partial cloning, tissue distribution and effects of epigallocatechin gallate on hepatic 3-hydroxy-3-methylglutaryl-CoA reductase mRNA transcripts in goldfish (Carassius auratus)

Cocci, Paolo; Mosconi, Gilberto; Palermo, Francesco Alessandro

doi:10.1016/j.gene.2014.05.030

Cited by 7 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five major catechin forms (catechin, C; epicatechin, EC; epigallocatechin, EGC; epicatechin gallate, ECG; and epigallocatechin gallate, EGCG) were detected in the PTAE. 27,28 In this study, gallic acid (43.48%) is the most abundant compound in the PTAE. 23 Several studies have shown that catechins are responsible for most of the beneficial health effects of green tea.…”

Section: Contents Of Ptaementioning

confidence: 80%

See 1 more Smart Citation

Effects of Pu-erh tea aqueous extract (PTAE) on blood lipid metabolism enzymes

et al. 2015

View full text Add to dashboard Cite

Disorders of blood lipid metabolism are the primary risk factors for many diseases. Recently, the effect of Pu-erh tea on blood lipid metabolism has received increasing attention. However, the mechanism underlying its ability to regulate blood lipid metabolism is unclear. We set out to study this through assessing the effects of Pu-erh tea aqueous extract (PTAE) on the central enzymes of blood lipid metabolism, including lipoprotein-associated phospholipase A2 (Lp-PLA2), lecithin: cholesterol acyltransferase (LCAT), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and pancreatic lipase (PL). We find that the Lp-PLA2, HMRG and PL activities are inhibited by PTAE in a dose-dependent manner and that the LCAT activity tends to increase with increasing PTAE concentrations. Lineweaver-Burk plot analyses reveal that PTAE acts as a competitive inhibitor for HMGR and PL and as a noncompetitive inhibitor for Lp-PLA2. Moreover, we determine that its active ingredients include catechins, gallic acid, caffeine, free amino acids, and soluble sugar. However, the effect of each ingredient and whether any of them have synergistic effects are still unknown. The results suggest that Pu-erh tea has a potent ability to regulate blood lipid metabolism and knowledge of the mechanisms provides insights into its potential therapeutic application as an alternative hypolipidemic drug.

show abstract

Section: Contents Of Ptaementioning

confidence: 80%

“…27,29 Therefore, these components are probably the major active ingredients in PTAE, especially gallic acid, which is the most abundant component. 27,29 Therefore, these components are probably the major active ingredients in PTAE, especially gallic acid, which is the most abundant component.…”

Section: Discussionmentioning

confidence: 99%

Effects of Pu-erh tea aqueous extract (PTAE) on blood lipid metabolism enzymes

et al. 2015

View full text Add to dashboard Cite

show abstract

“…HMG-CoA reductases, including HMG coenzyme A reductase a (hmgcra) and HMG coenzyme A reductase b (hmgcrb), are key enzymes in lipid metabolism and mainly regulate cholesterol biosynthesis genes (17,31). Fatty acid synthase ( fasn) regulates the synthesis and desaturation of fatty acids (19).…”

Section: Discussionmentioning

confidence: 99%

Naringenin inhibits alcoholic injury by improving lipid metabolism and reducing apoptosis in zebrafish larvae

et al. 2017

View full text Add to dashboard Cite

Alcoholic liver disease (ALD) includes a spectrum of hepatic abnormalities that range from isolated alcoholic steatosis to steatohepatitis and cirrhosis. Naringenin, a predominant flavanone in grapefruit, increases resistance to oxidative stress and inflammation and protects against multiple organ injury in various animal models. However, the specific mechanisms responsible for protection against alcoholic injury are poorly understood. In the present study, we aimed to investigate the effect of naringenin on alcoholic events and the molecular regulatory mechanisms of naringenin in the liver and whole body of zebrafish larvae following exposure to 350 mmol/l ethanol for 32 h. Zebrafish larvae {4 days post‑fertilization (dpf); wild-type (WT) and a transgenic line with liver-specific eGFP expression [Tg(lfabp10α-eGFP)]} were used to establish an alcoholic fatty liver model in order to evaluate the effects of naringenin treatment on anti-alcoholic injury. Naringenin significantly reduced alcoholic liver morphological phenotypes and the expression of alcohol and lipid metabolism-related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, fabp10α, fads2 and echs1, in zebrafish larvae. Naringenin also attenuated hepatic apoptosis in larvae as detected by TUNEL staining, consistent with the expression of critical biomarkers of endoplasmic reticulum stress and of DNA damage genes (chop, gadd45αa and edem1). The present study showed that naringenin inhibited alcohol-induced liver steatosis and injury in zebrafish larvae by reducing apoptosis and DNA damage and by harmonizing alcohol and lipid metabolism.

show abstract

“…HMG-CoA reductases are key enzymes in lipid metabolism, including HMG Coenzyme A reductase a (hmgcra), HMG Coenzyme A reductase b (hmgcrb) , and 3-hydroxy-3-methylglutaryl-CoA synthase (hmgcs) , mainly regulating genes related to cholesterol synthesis [14, 17, 28]. Besides, synthesis and desaturation of fatty acid can be regulated by fatty acid synthase (fasn) [19].…”

Section: Discussionmentioning

confidence: 99%

Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Zhou

Zhong

Lin

et al. 2017

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Alcoholic liver disease (ALD) is a series of abnormalities of liver function, including alcoholic steatosis, steatohepatitis, and cirrhosis. Hesperidin, the major constituent of flavanone in grapefruit, is proved to play a role in antioxidation, anti-inflammation, and reducing multiple organs damage in various animal experiments. However, the underlying mechanism of resistance to alcoholic liver injury is still unclear. Thus, we aimed to investigate the protective effects of hesperidin against ALD and its molecular mechanism in this study. We established an ALD zebrafish larvae model induced by 350 mM ethanol for 32 hours, using wild-type and transgenic line with liver-specific eGFP expression Tg (lfabp10α:eGFP) zebrafish larvae (4 dpf). The results revealed that hesperidin dramatically reduced the hepatic morphological damage and the expressions of alcohol and lipid metabolism related genes, including cyp2y3, cyp3a65, hmgcra, hmgcrb, fasn, and fads2 compared with ALD model. Moreover, the findings demonstrated that hesperidin alleviated hepatic damage as well, which is reflected by the expressions of endoplasmic reticulum stress and DNA damage related genes (chop, gadd45αa, and edem1). In conclusion, this study revealed that hesperidin can inhibit alcoholic damage to liver of zebrafish larvae by reducing endoplasmic reticulum stress and DNA damage, regulating alcohol and lipid metabolism.

show abstract

Partial cloning, tissue distribution and effects of epigallocatechin gallate on hepatic 3-hydroxy-3-methylglutaryl-CoA reductase mRNA transcripts in goldfish (Carassius auratus)

Cited by 7 publications

References 53 publications

Effects of Pu-erh tea aqueous extract (PTAE) on blood lipid metabolism enzymes

Effects of Pu-erh tea aqueous extract (PTAE) on blood lipid metabolism enzymes

Naringenin inhibits alcoholic injury by improving lipid metabolism and reducing apoptosis in zebrafish larvae

Hesperidin Protects against Acute Alcoholic Injury through Improving Lipid Metabolism and Cell Damage in Zebrafish Larvae

Contact Info

Product

Resources

About