Partial chemical characterization of Ia antigens derived from murine thymocytes.

Schwartz, Benjamin D.; Kask, Anne M.; Sharrow, Susan O.; David, Chella S.; Schwartz, Ronald H.

doi:10.1073/pnas.74.3.1195

Cited by 25 publications

(11 citation statements)

References 24 publications

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“…The I region of the major histocompatibility complex (MHC) codes for class I1 antigens that are crucially involved in cell communication processes during the immune response [l, 21. The I region of the rat MHC (RT1) exhibits a gene organization that closely resembles the one delineated for the mouse [3].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and cell surface display of class II determinants by long‐term propagated rat T line cells

et al. 1987

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We have investigated the capacity of the encephalitogenic BS rat T cell line bs 83 and its variant clone bs 83.III.C6 to synthesize and express RT1.B-specific class II molecule subsets defined by monoclonal antibodies (mAb) MRC-OX6 and MRC-OX3. Earlier studies had indicated that mAb MRC-OX6 recognizes three distinct molecular species: an immature oligomeric polypeptide chain complex comprised of the polymorphic subunits alpha, beta and the invariant proteins of the gamma group; a biosynthetic intermediate composed of post-translationally modified alpha, beta and gamma chain (denoted p35) and a fully glycosylated alpha, beta two-chain complex derived from the plasma membrane. MRC-OX3 was shown to recognize a serologically distinct alpha, beta two-chain complex that coexists with the MRC-OX6-specific heterodimer at the cell surface. Here we show that premutant bs 83 cells were unable to synthesize class II molecules of either set. In contrast endogeneous synthesis by mutant cells of MRC-OX6-specific molecules was demonstrated. Unlike control spleen cells variant cells failed to synthesize the mature MRC-OX3-reactive class II subset. Instead a three-polypeptide chain complex comprised of the terminally glycosylated subunits alpha, beta and invariant chain p35 was present at the cell surface. This complex appears to represent the preserved biosynthetic intermediate that failed to release invariant chain p35 upon its transit into the plasma membrane. These latter observations support our notion of gamma chain-induced epitope diversification during post-translational maturation of RT1.B-specific class II molecules.

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Section: Introductionmentioning

confidence: 99%

Synthesis and cell surface display of class II determinants by long‐term propagated rat T line cells

et al. 1987

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“…13. Figure 12 shows the results of our studies on murine thymocytes [48] and splenic macrophages (Cowing, Schwartz and Dickler, in press). Because of the apparent importance of the Ia antigens in the mediation of the immune response, and because several different types of immunocompetent cells are involved in this response, the structures of Ia antigens derived from various cell types have been examined.…”

Section: Peptide Mapping Studiesmentioning

confidence: 98%

“…Nonetheless, the agreement of the tryptic peptide mapping data [9,23] and the amino acid sequence data regarding homologous chains is reassuring. In the mouse, these cells include thymocytes [19,26,48], peritoneal exudate macrophages [19,55], and splenic macrophages (Cowing, Schwartz, and Dickler, in press), while in the guinea pig, T-lymphocytes, B-lymphocytes and peritoneal exudate macrophages have been studied [50]. Structure of Ia Antigens on Different Cell Types.…”

Section: Peptide Mapping Studiesmentioning

confidence: 99%

Chemical characteristics of Ia antigens

Schwartz

Cullen

1978

Springer Semin Immunopathol

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“…The expression of la antigens by mouse T cells, however, is still a matter of debate. While some reports describe the synthesis of la antigens by murine T cells [2, 14,23], other studies indicate that la antigens may be acquired by T cells passively from contaminating la-bearing non-T cells [15,17].…”

mentioning

confidence: 99%

Identification of Transcripts of the T Cell Antigen Receptor β Chain Gene and Major Histocompatibility Complex Class II Genes in Antigen‐Presenting Cloned BK‐BI‐2.6.C6 Cells

Rück

Steinlein

et al. 1988

Scand J Immunol

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The cloned murine cell line BK-BI-2.6.C6 has previously been shown to exhibit T cell characteristics, to synthesize and express MHC class II molecules, and to present protein antigens to antigen-dependent T cell clones. As a more definitive proof of the T-cell nature of these cells, transcripts of the rearranged T cell antigen receptor (TcR) beta gene were assessed by Northern blot analysis. BK-BI-2.6.C6 cells constitutively transcribe mRNA for the light chain of TcR and express the disulphide-linked alpha, beta TcR heterodimer at the cell surface. In addition mRNA for the polymorphic MHC class II subunits A alpha and A beta as well as for the invariant gamma chain were detected. BK-BI-2.6.C6 T cells effectively stimulated bovine insulin-reactive T hybridoma cells to lymphokine production in the presence of this antigen. Since the antigen-presenting and the responding T cell populations are maintained in culture in the absence of feeder cells, contamination by conventional accessory cells is excluded. These data unequivocally demonstrate that cloned murine Ia-expressing T cells can act as antigen-presenting accessory cells.

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Partial chemical characterization of Ia antigens derived from murine thymocytes.

Cited by 25 publications

References 24 publications

Synthesis and cell surface display of class II determinants by long‐term propagated rat T line cells

Synthesis and cell surface display of class II determinants by long‐term propagated rat T line cells

Chemical characteristics of Ia antigens

Identification of Transcripts of the T Cell Antigen Receptor β Chain Gene and Major Histocompatibility Complex Class II Genes in Antigen‐Presenting Cloned BK‐BI‐2.6.C6 Cells

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