Partial characterization of transferrins of some species of the family Cyprinidae

Stratil, A.; Bobj'ak, Petr; Valenta, M.; Tomášek, V.

doi:10.1016/0305-0491(83)90236-5

Cited by 13 publications

(13 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…approximate M r values of channid Tfs ( Figure 1B) are in agreement with the general range of 70-81 kD, documented for vertebrate Tfs (Stratil et al, 1983;Welch, 1990). Most of vertebrate serotransferrins are glycoproteins with the exceptions of some cyprinids among teleosts (Stratil et al, 1983).…”

Section: Discussionsupporting

confidence: 87%

“…Most of vertebrate serotransferrins are glycoproteins with the exceptions of some cyprinids among teleosts (Stratil et al, 1983). Purified Tf of each channid resembled teleost homologues as a monomeric glycoprotein (Stratil et al, 1985) and displayed no apparent interspecies differences of molecular weights (71-72 kD).…”

Section: Discussionmentioning

confidence: 99%

“…Purified Tf isoform was digested with neuraminidase according to the protocol of Stratil et al, (1983). The ratio of Tf to neuraminidase was 2:1 and the digests following 24 h of incubation were analyzed by SDS-PAGE outlined below.…”

Section: Neuraminidase Digestionmentioning

confidence: 99%

“…Transferrin is bilobal single polypeptide with each lobe having one iron binding domain (Baker et al, 2002) and M r of 70-80 kD (Stratil et al, 1983;1985). Tf is multifunctional protein which contributes to survival of fishes at various stages of life.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Untitled

Jabeen¹,

Nabi²,

Ahmad³

et al. 2015

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Channids are commercially important teleosts which can be cultured in shallow oxygen deficient waters due to accessory air-breathing. The information on metabolic plasticity of channids is inadequate; despite being fundamental to overall survival that determines their commercial output. To find out if transferrin (Tf) of genus Channa displays trends in functionality which correlate to their survival in shallow waters with low oxygen, we have compared iron-binding and pH dependent dispensation of bound iron in four channids (Channa punctatus, C. gachua and; C. striatus and C. marulius). Transferrin is central to iron metabolism as the main iron transporter and also intimately linked with oxygen-sensing. Our results show that their Tfs retain iron in exceptionally high amounts at acidic pH. Securing free iron at low pH should be imperative if respiratory acidosis occurs, since under low oxygen free iron (as Fe 3+ ) precipitates even at physiological pH. We suggest that retaining bound iron by Tfs at low pH values is a key factor in accomplishing iron homeostasis in channids with impact on fishery output.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Neuraminidase Digestionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Untitled

Jabeen¹,

Nabi²,

Ahmad³

et al. 2015

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…In mammals, TF is a glycoprotein with two N-glycosylation sites that are vital for iron transport (MacGillivray et al, 1982;O'Brien et al, 2007). However, the M. amblycephala TF lacked the potential N-glycosylation sites, which is in accord with that of other cyprinid fish (Dietrich et al, 2010;Stratil et al, 1983;Yang et al, 2004).…”

Section: Fig 2 Expression Of the M Amblycephala Tf (A) Andmentioning

confidence: 69%