Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata)

Nolasco, Héctor; Moyano, Francisco Javier; Vega-Villasante, Fernando

doi:10.1007/s10695-010-9414-7

Cited by 22 publications

(13 citation statements)

References 47 publications

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“…Divalent cations such as Ca 2+ are required to promote lipase activities in several fish species, such as gray mullet, Liza parsia (Islam et al ); tilapia, Oreochromis niloticus (Taniguchi et al ); and red sea bream, Pagrus major (Iijima et al ). However, no positive effects of Ca 2+ on lipase activity were observed in this study, which is similar to that reported for other fish lipases (Aryee et al ; Nolasco et al ). Striped bass activity increased when CaCl 2 was added to the incubation mixture with 100 mM NaCl without NaTC (77% of maximal), which represented a 50% relative increase compared with similar conditions without CaCl 2 .…”

Section: Discussionsupporting

confidence: 92%

“…Matus de la Parra et al () reported maximum activity at pH 9 for PBT, and Prasertsan and Prachumratana () reported an optimal pH at 10, which is in accordance with most lipase activity reports (Aloulou et al ). Consistent with these data, we found a marked loss in activity when the enzyme reactions were performed at pH 7.0 or lower; a similar tendency has been reported with different fish species (Iijima et al ; Aryee et al ; Prasertsan and Prachumratana ; Kurtovic et al ; Nolasco et al ).…”

Section: Discussionsupporting

confidence: 91%

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Biochemical Characterization and Comparison of Pancreatic Lipases from the Pacific Bluefin Tuna, Thunnus orientalis; Totoaba, Totoaba macdonaldi; and Striped Bass, Morone saxatilis

Rueda-López¹,

Martínez‐Montaño

Viana³

2016

J World Aquaculture Soc

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Lipolytic activity from pancreatic crude extracts from the Pacific bluefin tuna (PBT), Thunnus orientalis; totoaba, Totoaba macdonaldi; and striped bass, Morone saxatilis, were biochemically characterized to detect possible differences in the digestive capacity of each fish. The maximum activity was at temperatures ranging from 35 to 45 C and at pH 8.0; the activity decreased significantly at a pH lower than 7.0. Lipases from PBT and totoaba showed an increase in activity with the presence of sodium taurocholate (NaTC; 6 mM). However, no increase in activity could be observed when a mixture of bile salts or natural bile extracts was used in the reaction mixture, which was observed in the presence of NaTC. In contrast, the lipase activity from striped bass increased accordingly when NaCl was added at different levels (0–1.5 M). Calcium was not required in the reaction mixture for any of the fish species tested. We concluded that all three lipases have different physicochemical properties and ionic strength and emulsifier requirements to obtain their maximum activity. Striped bass was far too sensitive to NaCl, while totoaba and PBT were sensitive to NaTC. The differences in activity shown here suggest the possibility of studying the use of different additives on feed formulations to maximize lipase efficiency.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Biochemical Characterization and Comparison of Pancreatic Lipases from the Pacific Bluefin Tuna, Thunnus orientalis; Totoaba, Totoaba macdonaldi; and Striped Bass, Morone saxatilis

Rueda-López¹,

Martínez‐Montaño

Viana³

2016

J World Aquaculture Soc

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“…; Nolasco et al . ). Then, in the present study, an impairment in lipase activity would arise in animals fed acid oils, thus producing a lower fatty acids emulsification than in the other experimental oils and leading to a reduction in the absorption of fat.…”

Section: Discussionmentioning

confidence: 97%

“…; Nolasco et al . ). This enzyme is also present in mammals (Hui & Howles ) and shows strict dependence on bile salts for hydrolytic activity on insoluble lipid substrates (Gjellesvik et al .…”

Section: Introductionmentioning

confidence: 97%

Fatty acid digestibility in gilthead sea bream fed diets containing native, re-esterified or acid vegetable oils

et al. 2016

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Re‐esterified vegetable oils are obtained from a chemical esterification reaction between vegetable acid oils and glycerol. Due to their properties, it is expected that they have a higher nutritive value than their corresponding acid oils and a better digestibility than their native counterparts. The aim of this study was to determine the effect of re‐esterified oils with a different monoacylglycerol (MAG) and diacylglycerol (DAG) content, produced from palm or rapeseed, on fatty acid digestibility in gilthead sea bream (Sparus aurata). Triplicate groups of fish were fed nine experimental diets containing different oils during 28 days. For each source, four different types of oil were used: native, re‐esterified low or high in MAG and DAG and acid. A commercial fish oil was used for the control diet. Diets containing re‐esterified oils had better apparent digestibility coefficients (ADC) of total fatty acids than acid oil diets. Re‐esterified oils do not negatively affect apparent digestibility coefficients of fatty acids when compared to their corresponding native oils and could be incorporated as a source of energy in diets for gilthead sea bream. An improvement in digestibility compared to the native oil diet was only obtained in palm re‐esterified oil high in MAG and DAG.

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“…Optimal conditions for sex-specific lipase isoforms were reported by Areekijseree et al (2002) and Thongprajukaew et al (2010b). Other lipase isoforms with different optimal conditions were reported in many aquatic species, such as pH 8.5 and 35°C to 40°C for juvenile red claw crayfish C. quadricarinatus (López-López et al 2003), pH 8.0 to 8.5 and 33°C to 35°C for grey mullet Liza parsia (Islam et al 2008), and pH 8.5 and 50°C for gilthead seabream S. aurata (Nolasco et al 2011).…”

Section: Characterization Of Digestive Enzymesmentioning

confidence: 99%

Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Thongprajukaew

Kovitvadhi

2013

Zool. Stud.

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Background: Characteristics (pH 2 to 12 and temperature of 25°C to 80°C) and expression levels of the main digestive enzymes, including amylase, lipase, total protease, trypsin, and chymotrypsin, were investigated in adult male and female guppies Poecilia reticulata. A completely randomized design was used during this experiment. Results: Digestive enzyme studies indicated that sex had a direct effect on characteristic profiles (n = 3) and expression levels (n = 10) of all digestive enzymes. The most suitable conditions for studying digestive enzymes in general were pH 2 at 50°C for acidic amylase, pH 7 at 55°C for neutral amylase, pH 11 at 50°C for alkaline amylase, pH 7 at 30°C to 35°C for lipase, pH 2 at 55°C for acidic protease, pH 9 at 45°C for alkaline protease, pH 8 at 50°C for trypsin, and pH 9 at 50°C for chymotrypsin, regardless of sex. Specific activities of amylase, total protease, trypsin, and chymotrypsin were higher in females than males (p < 0.001), while the specific activity of lipase was higher in males than females. These findings were correlated with the higher growth capacity of females than males (except for lipase), as indicated by differences in body weight (p < 0.001), length (p < 0.001), and the activity ratio of trypsin (T) to chymotrypsin (C), i.e., the T/C ratio (p < 0.007).

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Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata)

Cited by 22 publications

References 47 publications

Biochemical Characterization and Comparison of Pancreatic Lipases from the Pacific Bluefin Tuna, Thunnus orientalis; Totoaba, Totoaba macdonaldi; and Striped Bass, Morone saxatilis

Biochemical Characterization and Comparison of Pancreatic Lipases from the Pacific Bluefin Tuna, Thunnus orientalis; Totoaba, Totoaba macdonaldi; and Striped Bass, Morone saxatilis

Fatty acid digestibility in gilthead sea bream fed diets containing native, re-esterified or acid vegetable oils

Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

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