Partial characterization of giant extracellular hemoglobin of Glossoscolex paulistus: A MALDI-TOF-MS study

Ferric species equilibrium of the giant extracellular hemoglobin of Glossoscolex paulistus in alkaline medium: HALS hemichrome as a precursor of pentacoordinate species

“…In a recent work, the amino acid sequences of the four kinds of "Linker" chains (L1, L2, L3 and L4) have been determined [17]. HbGp is also constituted by a large number of subunits containing heme groups with molecular weights in the range of 16-19 kDa forming a monomer of 16 kDa and a trimer of 52 kDa and non-heme structures with molecular weights in the range 24-32 kDa [18], conferring to the whole protein a minimum molecular weight of 3100 kDa and a double layered hexagonal oligomeric structure [19,20].…”

Giant Extracellular Hemoglobin of Glossoscolex paulistus: An excellent Prototype of Biosensor and Blood Substitute

“…This point is very interesting, since the hemes present a very similar behavior in terms of ligand affinity and spectroscopic profile to the native form of HbGp, in spite of their significant structural differences. This distinction can be observed in the recent studies employing MALDI-TOF-MS, which denote the great number of coexistent isoforms in each subunit (Oliveira et al, 2007;Oliveira et al, 2008). In fact, the oligomeric compaction of the native form works as a kind of equalization of the different hemes.…”

Ferric species of the giant extracellular hemoglobin of Glossoscolex paulistus as function of pH: An EPR study on the irreversibility of the heme transitions

“…The hemoglobin of Glossoscolex paulistus (HbGp) belongs to the same class of the HbLt and exhibits similar structural characteristics to this hemoglobin (Oliveira et al, 2007(Oliveira et al, , 2008. G. paulistus is an annelid found in Araras, Rio Claro and Piracicaba, which are cities of the São Paulo state in Brazil (Righi, 1972).…”