Partial characterization of ethanolic extract of Melipona beecheii propolis and in vitro evaluation of its antifungal activity

Ramón-Sierra, Jesús; Peraza-López, Enrique; Rodríguez-Borges, Raquel; Yam-Puc, Alejandro; Madera-Santana, Tomás J.; Ortiz-Vázquez, Elizabeth

doi:10.1016/j.bjp.2019.04.002

Cited by 22 publications

(15 citation statements)

References 28 publications

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“…A study on Malaysian stingless bee propolis extracts, including T. apicalis extract showed a significant amount of total flavonoids, which are important in exhibiting potent antioxidant activity ( Asem et al 2019 ). Another study conducted on total flavonoids contents of Mexican Melipona beecheii and A. mellifera propolis extract yielded 7.68 and 17.23 mg/g, respectively ( Ramón-Sierra et al 2019 ). Variations observed in the amount of flavonoids found in the stingless bee propolis could be attributed to differences in plant preferences as well as vegetations pollinated by the bees ( Rosli et al, 2016 , Awang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts

Salleh

Hanapiah

Johari

et al. 2021

Saudi Journal of Biological Sciences

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Propolis is a resinous substance collected by stingless bees containing bioactive compounds which exert various biological properties. The present study focused on the evaluation of chemical profiles produced by three Indo-Malayan stingless bee propolis extracted using water. Fresh propolis was collected from the same area and ecosystem conditions in Selangor, Malaysia, namely Tetrigona apicalis , Tetrigona binghami , and Heterotrigona fimbriata . The bioactive compounds and chemical composition of propolis extracts were then analyzed using gas chromatography–mass spectrometry (GC–MS). Results showed that propolis from the three different stingless bee species consisted of major groups such as sugar (31.4%), carboxylic acid (17.1%), terpenoid (14.3%), sugar alcohol (11.4%), hydrocarbon (5.7%), aldehyde (5.7%) amino acid (2.9%) and other constituents (11.4%). Heterotrigona fimbriata displayed the highest amount for both total phenolics (13.21 mg/mL) and flavonoids (34.53 mg/mL) compared to other propolis extracts. There is also no significant difference detected between all samples since p ≤ 0.05. In conclusion, this study shows that Malaysian stingless bee propolis contain bioactive components that have great potential to be used for their therapeutic and medicinal benefits. However, more investigations and analysis of stingless bee propolis need to be carried out in order to enhance the understanding and applications of propolis in the future.

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Section: Discussionmentioning

confidence: 99%

Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts

Salleh

Hanapiah

Johari

et al. 2021

Saudi Journal of Biological Sciences

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“…The fungal suspension (1 mL) was dissolved in Mueller-Hinton agar medium and distributed into Petri dishes, and the wells were made. PP (0.1 g) and PNE (0.1 mL) were added to the wells and left for 48 h. Their effect was evaluated by the diameter of the formed zone around each well [27]. Positive control plates were used without any antifungal compounds.…”

Section: Effects Of Pp and Pne On A Flavus Growthmentioning

confidence: 99%

Antifungal and antitoxin effects of propolis and its nanoemulsion formulation against Aspergillus flavus isolated from human sputum and milk powder samples

et al. 2021

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Background and Aim: Aspergillus flavus causes human and animal diseases through either inhalation of fungal spores or ingestion of mycotoxins as aflatoxins produced in human and animal feed as secondary metabolites. This study was aimed to detect the incidence of A. flavus and its aflatoxins in human sputum and milk powder samples and explore the efficacy of pure propolis (PP) and propolis nanoemulsion (PNE) as natural decontaminants against fungal growth and its released aflatoxins. Materials and Methods: A. flavus was isolated by mycological culture and identified macroscopically and microscopically. Coconut agar medium and thin-layer chromatography (TLC) were used to qualitatively detect aflatoxins in the isolated strains. Toxins were extracted from toxigenic strains by the fast extraction technique. The quantitative detection of toxin types was explored by high-performance liquid chromatography (HPLC). PNE was prepared by a novel method using natural components and characterized by Fourier-transform infrared spectroscopy, Zetasizer, and transmission electron microscopy. The effects of PP and PNE on A. flavus growth and its toxin were determined by the well-diffusion method and HPLC. Results: The mycological culture showed that 30.9% and 29.2% of sputum and milk powder samples were positive for A. flavus, respectively. TLC confirmed the production of 61.8% and 63.2% aflatoxin by the isolated strains in sputum and milk powder, respectively. PP and PNE showed antifungal activity on A. flavus growth with mean±standard error (SE) inhibition zones of 27.55±3.98 and 39.133±5.32 mm, respectively. HPLC revealed positive contamination of toxin extracts with AFB1, AFB2, and AFG2 at 0.57±0.026, 0.28±0.043, and 0.1±0.05 mg/L, respectively. After treatment with PP and PNE, a significant decrease in AFB1, AFB2, and AFG2 concentrations was observed. Conclusion: This study suggested using propolis and its nanoformulation as antifungal and antitoxins in human medicine and the food industry to increase the food safety level and stop food spoilage.

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“…Furthermore, the antibacterial and antifungal activity of bee propolis has been attributed, at least partially, to its phenolic content (flavonoids, phenolic acids, and their esters), such as the flavonoid galangin (25, 63) ( Fig. 2), although the composition of the propolis varies depending on the plant and bee species of each location (72).…”

Section: Polyphenolsmentioning

confidence: 99%

Plant Phytochemicals in Food Preservation: Antifungal Bioactivity: A Review

Redondo-Blanco

Fernández

López-Ibáñez

et al. 2020

Journal of Food Protection

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Synthetic food additives generate a negative perception in consumers. This fact generates an important pressure on food manufacturers, searching for safer natural alternatives. Phytochemicals (such as polyphenols and thiols) and plant essential oils (terpenoids) possess antimicrobial activities that are able to prevent food spoilage due to fungi (e.g., Aspergillus, Penicillium) and intoxications (due to mycotoxins), both of which are important economic and health problems worldwide. This review summarizes industrially interesting antifungal bioactivities from the three main types of plant nutraceuticals: terpenoids (as thymol), polyphenols (as resveratrol) and thiols (as allicin) as well as some of the mechanisms of action. These phytochemicals are widely distributed in fruits and vegetables and are very useful in food preservation as they inhibit growth of important spoilage and pathogenic fungi, affecting especially mycelial growth and germination. Terpenoids and essential oils are the most abundant group of secondary metabolites found in plant extracts, especially in common aromatic plants, but polyphenols are a more remarkable group of bioactive compounds as they show a broad array of bioactivities. HIGHLIGHTS

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Partial characterization of ethanolic extract of Melipona beecheii propolis and in vitro evaluation of its antifungal activity

Cited by 22 publications

References 28 publications

Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts

Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts

Antifungal and antitoxin effects of propolis and its nanoemulsion formulation against Aspergillus flavus isolated from human sputum and milk powder samples

Plant Phytochemicals in Food Preservation: Antifungal Bioactivity: A Review

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