Partial Characterization and Molecular Cloning of a Closterovirus from Sweet Potato Infected with the Sweet Potato Virus Disease Complex from Nigeria

The closteroviruses, capilloviruses and other similar viruses: a short review

“…(b) PAGE (Dodds & Bar-Joseph, 1983). associated with pineapple wilt, a virus associated with sweet potato virus disease, grapevine leafroll virus, lettuce infectious yellows virus and BPYV (Namba et al, 1991 ;Gunasinghe & German, 1989;Winter et al, 1992;Hu et al, 1990;Larsen et aL, 1991;Coffin & Coutts, 1990). Extracts of these plants all contain one high Mr dsRNA species and in some cases a number of species of lower M r in a pattern not dissimilar to that found with BYV, CTV or CNFV infections.…”

“…Of the capilloviruses, the full sequence of ASGV is now known (Yoshikawa et al, 1992). Clones have also been generated specific to the U.K. isolate of beet pseudoyellows virus (BPYV; Coffin & Coutts, 1992) and to a closterovirus associated with sweet potato virus disease (Winter et al, 1992), but these sequences have yet to be reported. As might have been anticipated, the sequences of ACLV and ASGV, although at present tentatively placed in separate virus groups, show distinct similarities, as do the sequences of BYV and CTV (see Fig.…”

Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus

“…Sweet potato chlorotic stunt virus (SPCSV; previously also known as sweet potato sunken vein virus) belongs to the genus Crinivirus in the family Closteroviridae and is a pathogen of sweet potato (Ipomoea batatas L.) (23,35,61). It is a required component for the manifestation of several severe viral diseases caused by coinfection with other viruses (9,13,23).…”

Extracción y purificación de dsRNAs largos de plantas infectadas con virus y hongos; aplicación en la detección e identificación de virus

“…After heat denaturation, purified viral dsRNA has been used extensively as the template for reverse transcription PCR (RT-PCR) in plant virus testing (Rott and Jelkmann, 2001;Sabanadzovic and Valverde, 2011;Valverde and Sabanadzovic, 2009;Valverde et al, 2011;Tzanetakis et al 2005). Winter et al (1992) and Hoyer et al (1996) characterized Sweet potato chlorotic stunt virus using dsRNA as reagent for RT-PCR, cloning, and labeling. Zhang and Rowhani (2000) developed a rapid cDNA cloning method from dsRNA templates to partially characterized grape viruses.…”

“…Después de someterlo a desnaturalización por calor, el dsRNA viral purificado se ha utilizado extensamente como molde para la transcripción reversa de PCR (RT-PCR) en pruebas con fitovirus (Rott and Jelkmann, 2001;Sabanadzovic y Valverde, 2011;Valverde y Sabanadzovic, 2009;Valverde et al, 2011;Tzanetakis et al 2005). Winter et al (1992) y Hoyer et al (1996) caracterizaron el Virus del enanismo del camote utilizando un reactivo para la RT-PCR, la clonación y el etiquetado. Zhang y Rowhani (2000) desarrollaron un método rápido de clonación de cDNA a partir de moldes de dsRNA para caracterizar parcialmente virus de uva.…”