Partial characterization and antioxidant activity of exopolysaccharides produced by Lactobacillus plantarum CNPC003

Bomfim, Vanessa Barbosa; Neto, José Honório Pereira Lopes; Leite, Kerolayne Santos; Vieira, Érica de Andrade; Iacomini, Marcello; Silva, Caroline Mellinger; Santos, K. M. O. dos; Cardarelli, Haíssa Roberta

doi:10.1016/j.lwt.2020.109349

Cited by 57 publications

(39 citation statements)

References 42 publications

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“…Since FOS are also composed of saccharides, their structure has much in common with the matrix polysaccharides. Indeed, some of the above-mentioned bands have previously been detected as part of FOS spectra, as the ones at 1055 (Bomfim et al, 2020), 1030, and 986 cm −1 (Romano, Santos, Mobili, Vega, & Gómez-Zavaglia, 2016). So, in FOScontaining films, many bands originate from both matrix polysaccharides and FOS, as also observed in other studies with films containing oligosaccharides (Bersaneti et al, 2016;Orozco-Parra et al, 2020).…”

Section: Ftir Spectrasupporting

confidence: 71%

Bacterial cellulose/cashew gum films as probiotic carriers

Oliveira-Alcântara

Abreu

Gonçalves

et al. 2020

LWT

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This study was carried out to obtain probiotic films with good stability by combining spore-forming, resistant bacteria (Bacillus coagulans) with a biopolymer mix (bacterial cellulose-BC and cashew gum-CG) as a carrier matrix. Fructooligosaccharides (FOS) were used as prebiotic. Four different films were produced, namely, Co (control), Pro (added with probiotic), Pre (containing the prebiotic FOS), and Syn (synbiotic films containing probiotic and FOS). Although the tensile and barrier properties of films have been undermined by probiotic and FOS, those properties have remained within the values needed for food applications. Most films (except Pre) exhibited hydrophobic character (contact angles > 90°). FOS enhanced probiotic viability upon processing. The storage stability of probiotics was very good; even at 37°C, the viability loss did not surpass 1 log cycle, due to the resistance of B. coagulans and the protective role of BC. Moreover, no cytotoxic effect of the films was observed on Caco-2 cells.

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Section: Ftir Spectrasupporting

confidence: 71%

Bacterial cellulose/cashew gum films as probiotic carriers

Oliveira-Alcântara

Abreu

Gonçalves

et al. 2020

LWT

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“…Some evidence suggests that L. plantarum may produce antioxidant substances, including polysaccharides, proteins, vitamins and glutathione [20], and the antioxidant enzymes contained in its own antioxidant system [53,54]. The extensively studied exopolysaccharides have a strong ability to scavenge free radicals [55], and they can reduce the accumulation of lipid peroxides by stimulating the activity of antioxidant enzymes in mice, and ultimately reduce the oxidative stress in mice [56]. Therefore, L. plantarum ATCC8014 might produce polysaccharides, proteins and other substances, which can stimulate the antioxidant system of rats to enhance their own antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

Zhao

Liu

et al. 2020

Appl Biol Chem

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Acrylamide (AA), which is mainly found in fried foods, causes neurotoxicity, genetic toxicity, carcinogenic effects, and DNA damage. This study confirms that a strain of lactic acid bacteria (Lactobacillus plantarum ATCC8014) could alleviate the toxicity of rats by inhibiting the AA-induced oxidative damage. Forty-eight adult male SD rats were randomly divided into eight groups: control group, AA group (40 mg/kg), three different doses (1 × 10 7 CFU/ml, 1 × 10 8 CFU/ ml, 1 × 10 9 CFU/ml of Lactobacillus plantarum ATCC8014) of prevention groups and therapeutic groups, respectively. At the end of three-week experiment, AA treatment produced a significant reduction in the rate of weight gain along with the symptoms of hind limb splay and ataxia. Histological examinations revealed various degrees of injury in five tissues. Levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) in group AA rats were significantly decreased, but the level of lipid peroxidation (LPO) was significantly increased (p < 0.05). Both prevention and therapeutic groups with 1 × 10 9 CFU/ml of Lactobacillus plantarum ATCC8014 could effectively reduce the injury of AA to the body. However, reductions in both groups were not statistically significant.

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“…Antimicrobial activity of LN66 CFS was evaluated by agar-well diffusion assay, five pathogens, H. pylori ATCC43504, E. coli ATCC25922, S. aureus ATCC29213, S. sonnei ATCC25931, S. tyhimurium ATCC14028 were used as indicator strains. The protein content of LN66 CFS was measured with a Bradford protein assay kit (Sangon, Shanghai, China), the polysaccharide content was measured by the phenol-sulfate method, and the pH value was measured [ 13 , 14 ]. L. rhamnosus GG, L. paracasei ATCC334, L. reuteri DSM20016 were used as positive controls for the above assays.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Effects of Lactobacillus plantarum LN66 and Antibiotics Used Alone or in Combination on Helicobacter pylori Mature Biofilm

Yang

2021

Microorganisms

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Helicobacter pylori is a gastrointestinal pathogen with high prevalence that harms human health. Studies have shown that H. pylori can form antibiotic-tolerant biofilms, which may interfere with the efficacy of clinical antibiotic therapy. Probiotics can antagonize planktonic and biofilm pathogen cells and thus may play an auxiliary role in H. pylori antibiotic therapy. However, the effects of different probiotic strains and antibiotic combinations on H. pylori biofilms need to be further investigated. We determined the cell viability of H. pylori mature biofilms after treatment with Lactobacillus plantarum LN66 cell-free supernatant (CFS), clarithromycin (CLR), and levofloxacin (LVX) alone or in combination by the XTT method. Biofilm cells were observed by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Subsequently, protein and polysaccharide concentrations in biofilm extracellular polymeric substances (EPSs) were quantitatively detected by the Bradford method and the phenol-sulfate method. The results showed that LN66 CFS had an eradication effect on mature H. pylori biofilm. When used in combination with CLR, LN66 CFS significantly attenuated the eradication effect of CLR on biofilms; in contrast, when used in combination with LVX, LN66 CFS enhanced the disrupting effect of LVX. We speculate that the different effects of CFS and antibiotic combinations on biofilms may be related to changes in the content of proteins and polysaccharides in EPS and that the combination of CFS and CLR might promote the secretion of EPS, while the combination of CFS and LVX might have the opposite effect. Accordingly, we suggest that supplementation with L. plantarum LN66 may provide additional help when therapy involving LVX is used for clinical H. pylori biofilm eradication, whereas it may impair CLR efficacy when therapy involving CLR is used.

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Partial characterization and antioxidant activity of exopolysaccharides produced by Lactobacillus plantarum CNPC003

Cited by 57 publications

References 42 publications

Bacterial cellulose/cashew gum films as probiotic carriers

Bacterial cellulose/cashew gum films as probiotic carriers

Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

In Vitro Effects of Lactobacillus plantarum LN66 and Antibiotics Used Alone or in Combination on Helicobacter pylori Mature Biofilm

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