Partial body irradiation of small laboratory animals with an industrial X-ray tube

Frenzel, Thorsten; Grohmann, Carsten; Schumacher, Udo; Krüll, Andreas

doi:10.1016/j.zemedi.2014.02.002

Cited by 10 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Equivalent to locally ablative radiotherapy in humans, RT was administered in five fractions of 10 Gy on consecutive days with an X-ray tube (Rich. Seifert & Co. GmbH & Co. KG, Ahrensburg, Germany) [ 19 ]. ChT was applied as an intraperitoneal injection of cisplatin (6 mg/kg) on the same day as radiotherapy commenced.…”

Section: Methodsmentioning

confidence: 99%

Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundTumor vasculature is critical for tumor growth, formation of distant metastases and efficiency of radio- and chemotherapy treatments. However, how the vasculature itself is affected during cancer treatment regarding to the metastatic behavior has not been thoroughly investigated. Therefore, the aim of this study was to analyze the influence of hypofractionated radiotherapy and cisplatin chemotherapy on vessel tree geometry and metastasis formation in a small cell lung cancer xenograft mouse tumor model to investigate the spread of malignant cells during different treatments modalities.MethodsThe biological data gained during these experiments were fed into our previously developed computer model “Cancer and Treatment Simulation Tool” (CaTSiT) to model the growth of the primary tumor, its metastatic deposit and also the influence on different therapies. Furthermore, we performed quantitative histology analyses to verify our predictions in xenograft mouse tumor model.ResultsAccording to the computer simulation the number of cells engrafting must vary considerably to explain the different weights of the primary tumor at the end of the experiment. Once a primary tumor is established, the fractal dimension of its vasculature correlates with the tumor size. Furthermore, the fractal dimension of the tumor vasculature changes during treatment, indicating that the therapy affects the blood vessels’ geometry. We corroborated these findings with a quantitative histological analysis showing that the blood vessel density is depleted during radiotherapy and cisplatin chemotherapy. The CaTSiT computer model reveals that chemotherapy influences the tumor’s therapeutic susceptibility and its metastatic spreading behavior.ConclusionUsing a system biological approach in combination with xenograft models and computer simulations revealed that the usage of chemotherapy and radiation therapy determines the spreading behavior by changing the blood vessel geometry of the primary tumor.

show abstract

Section: Methodsmentioning

confidence: 99%

Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The animals were treated by local radiation therapy, 6 chemotherapy, radiochemotherapy, or no therapy at all as a control group. At the end of the experiments up to 13 different samples were taken from each animal for further analyses.…”

Section: Animals Materials and Methodsmentioning

confidence: 99%

Monitoring of small laboratory animal experiments by a designated web-based database

et al. 2015

View full text Add to dashboard Cite

Multiple-parametric small animal experiments require, by their very nature, a sufficient number of animals which may need to be large to obtain statistically significant results.(1) For this reason database-related systems are required to collect the experimental data as well as to support the later (re-) analysis of the information gained during the experiments. In particular, the monitoring of animal welfare is simplified by the inclusion of warning signals (for instance, loss in body weight >20%). Digital patient charts have been developed for human patients but are usually not able to fulfill the specific needs of animal experimentation. To address this problem a unique web-based monitoring system using standard MySQL, PHP, and nginx has been created. PHP was used to create the HTML-based user interface and outputs in a variety of proprietary file formats, namely portable document format (PDF) or spreadsheet files. This article demonstrates its fundamental features and the easy and secure access it offers to the data from any place using a web browser. This information will help other researchers create their own individual databases in a similar way. The use of QR-codes plays an important role for stress-free use of the database. We demonstrate a way to easily identify all animals and samples and data collected during the experiments. Specific ways to record animal irradiations and chemotherapy applications are shown. This new analysis tool allows the effective and detailed analysis of huge amounts of data collected through small animal experiments. It supports proper statistical evaluation of the data and provides excellent retrievable data storage.

show abstract

“…A secondary collimation, obtained with 10 mm thick, custom-made MCP 96 blocks, provides quadratic and rectangular field sizes between (10 × 10) and (80 × 80) mm 2 . Their dosimetric properties were characterized in a previous work in terms of dose to water traceable to primary standards (Frenzel et al 2014), so that look-up tables are available for each field size. Also in this case, we delivered dose to several target volumes (see table 2) with a single dorsal field after fixing the phantom to the mouse treatment couch.…”

Section: Irradiation Geometries and Qualitiesmentioning

confidence: 99%

“…Furthermore, we compared the absolute dose on the field central axis at 4 mm depth. This is the point at which the dose output factors were previously determined (Frenzel et al 2014).…”

Section: Dose Calculationsmentioning

confidence: 99%

See 1 more Smart Citation

Development and characterization of modular mouse phantoms for end-to-end testing and training in radiobiology experiments

Wegner

Frenzel²,

Krause³

et al. 2023

Phys. Med. Biol.

Self Cite

View full text Add to dashboard Cite

Objective. In radiation oncology, experiments are often carried out using mice as a model for in-vivo research studies. Due to recent technological advances in the development of high-precision small-animal irradiation facilities, the importance of quality assurance for both dosimetry and imaging is increasing. Additive manufacturing (AM) offers the possibility to produce complex models from a three-dimensional data set and to build cost-effective phantoms that can easily be adapted to different purposes. The aim of this work was therefore to develop detailed anatomical mouse models for quality assurance and end-to-end testing of small-animal irradiation and imaging by means of AM. Approach. Two mouse phantom concepts were designed, constructed, and examined for this purpose. The first model includes cavities corresponding to the most important organs. The final solid model was constructed using AM in two separate parts that can be attached with a plug connection after filling these cavities with tissue-equivalent mixtures. Moreover, different radiation dosimeters can be placed in the lower part of the model. For the second concept, AM was used for building modules like the phantom outer shell and bones, so that different mixtures can be used as a filling, without modifying the phantom structure. Main results. CT as well as Micro-CT scans of both concepts showed an excellent quality and adequate image contrast, with material attenuation properties close to those of mouse tissues, apart from the current bone surrogates. Radiation dose measurements with radiochromic films were, with some exceptions in areas with larges bone volumes, in agreement with calculations within less than ± 4 %. Significance. AM shows great potential for the development of mouse models that are inexpensive, easy to adapt, and accurate, thus enabling their use for quality assurance in small-animal radiotherapy and imaging. The introduction of such 3D-printable mouse phantoms in the workflow could also significantly reduce the use of living animals for optimization and testing of new imaging and irradiation protocols.

show abstract

Partial body irradiation of small laboratory animals with an industrial X-ray tube

Cited by 10 publications

References 24 publications

Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model

Radiotherapy and chemotherapy change vessel tree geometry and metastatic spread in a small cell lung cancer xenograft mouse tumor model

Monitoring of small laboratory animal experiments by a designated web-based database

Development and characterization of modular mouse phantoms for end-to-end testing and training in radiobiology experiments

Contact Info

Product

Resources

About