Partial amino acid sequence of a rabbit immunoglobulin light chain of allotype b5

Ayadi, Hammadi; Dutka, Sophie; Paroutaud, Pierre; Ad, Strosberg

doi:10.1021/bi00273a044

Cited by 4 publications

(1 citation statement)

References 24 publications

(39 reference statements)

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“…In the two b9 clones subjected to sequence analysis so far, two different JKs have been found. A unique expressed JK has been found to date in bS proteins and cDNAs (18,35) that differs from the five genomic JK genes associated with b4 CK (25) and the three associated with bas-N4 (10). It is evident in comparing the rabbit CDR3 and JK that there are large length differences.…”

Section: Methodsmentioning

confidence: 99%

Kappa-chain allotypes and isotypes in the rabbit: cDNA sequences of clones encoding b9 suggest an evolutionary pathway and possible role of the interdomain disulfide bond in quantitative allotype expression.

McCartney-Francis¹,

Skurla²,

Mage³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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The constant regions of rabbit K light chains are unusual because the sequences of the allotypic forms can differ more from each other than do some variable regions with which they associate. We report the nucleic acid sequence of a full-length cDNA clone of b9 allotype and show comparisons to available sequences of the rabbit K allotypes b, b5, and bas-N4. Our analyses suggest that the primordial rabbit K gene encoded a bas-like sequence. They also reveal a surprising difference in the position of the variable region cysteine that forms the interdomain disulfide bond that is unique to most rabbit K chains. One b9 cDNA sequence lacks the usual cysteine-80 and instead encodes cysteine-108, which in three-dimensional models appears capable of forming the interdomain disulfide bond with cysteine-171 in the constant region. A partial sequence of a second b9 clone encodes both cysteine-80 and cysteine-108; the translation product of this clone could have a free reactive sulfitydryl group that might lead to an unstable nonfunctional Ig molecule. The fact that pre-B cells with b9 K chains do not differentiate and expand into productive Ig-producing cells with frequencies comparable to the other allotypes may be explained if a substantial proportion of the gene products have a free sulfhydryl group. Our sequence results suggest that in cells differentiating to produce K light chains of b9 allotype the number and location of the cysteines influence immunoglobulin expression.

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Section: Methodsmentioning

confidence: 99%

Kappa-chain allotypes and isotypes in the rabbit: cDNA sequences of clones encoding b9 suggest an evolutionary pathway and possible role of the interdomain disulfide bond in quantitative allotype expression.

McCartney-Francis¹,

Skurla²,

Mage³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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Genomic Evidence for the Truly Allelic Nature of the B-Locus Polymorphism of the Rabbit Immunoglobulin Light Chain

Matthyssens

Borremans

Hamers

et al. 1985

Protides of the Biological Fluids

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A genomic gene encoding the b5 rabbit immunoglobulin kappa constant region: implications for latent allotype phenomenon.

Emorine¹,

Sogn²,

Trinh³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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We previously reported that domestic rabbits of five immunoglobulin K allotype strains (b4', b4, b5, b6, and b9) harbor at least two DNA sequences that hybridize strongly to K constant region probes in Southern blots. One of these sequences ("type A") has been identified as encoding the constant region of the K2 isotype, an immunoglobulin chain that most rabbits express only at low levels, if at all. We identified the second sequence-for rabbits of the b4 allotype-as encoding the nominal b4 K chain (or K1 isotype), but for rabbits of other allotypes no definite identification for this "type B" sequence could be made. Here we suggest that the type B sequence in rabbits of the other domestic allotypes also encodes the nominal K1 immunoglobulin chain. We show this directly for the b5 allotype; a type B sequence cloned from b5 DNA has been found to contain an apparently functional gene encoding the b5 constant region sequence. Indirect arguments suggest the corresponding conclusion for the b'V, b6, and b9 allotypes. We have considered the implications of these results for the phenomenon of "latent allotype" expression.The immunoglobulin K gene system of rabbits is more complex than the well-studied homologous loci of mouse and man in that rabbits demonstrate several allotypic variants of "b series" K chains (known as b4v, b4, b5, b6, and b9 in domestic rabbits) as well as an additional K isotype (known as K2 or "bas") that is generally expressed at very low levels. The inheritance of b series allotype expression is not completely understood (reviewed in ref. 1). Although initial studies indicated that allotypes were controlled by codominantly expressed allelic structural genes, this model was challenged by serologic detection in some rabbits of "latent" allotypes-i.e., K chains unpredicted by pedigree of the rabbits. The phenomenon of latent allotypes suggested that rabbits might harbor structural genes for more K chains than are normally expressed. The usual inheritance of "nominal" allotype expression might then result from an inherited regulatory mechanism that would select the allotype to be expressed; latent allotype expression could result from "leakage" of the regulatory mechanism.We have been pursuing the molecular basis for latent allotype expression by investigating the K chain gene system of rabbits. We recently cloned the nominal b4 germline constant K (CK) gene (designated Klb4) from a b4 rabbit and showed by Southern blot analyses that rabbits of five different b series pedigrees each harbor two sequences strongly homologous to the cloned b4 CK gene (2); the two sequences were arbitrarily designated type A and type B. Subsequent work from our laboratory (3) and from Heidmann and Rougeon (4) identified the type A sequence of a b4 rabbit as encoding the K2 isotype; we refer to this gene as K2b4. By inference, the type A sequences in b4v, b5, b6, and b9 rabbits probably also represent K2 isotype genes.The identity of the type B sequences has been clearly established only in b4 rabbits, where the B...

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Partial amino acid sequence of a rabbit immunoglobulin light chain of allotype b5

Cited by 4 publications

References 24 publications

Kappa-chain allotypes and isotypes in the rabbit: cDNA sequences of clones encoding b9 suggest an evolutionary pathway and possible role of the interdomain disulfide bond in quantitative allotype expression.

Kappa-chain allotypes and isotypes in the rabbit: cDNA sequences of clones encoding b9 suggest an evolutionary pathway and possible role of the interdomain disulfide bond in quantitative allotype expression.

Genomic Evidence for the Truly Allelic Nature of the B-Locus Polymorphism of the Rabbit Immunoglobulin Light Chain

A genomic gene encoding the b5 rabbit immunoglobulin kappa constant region: implications for latent allotype phenomenon.

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