Parthenolide inhibits lipid accumulation via activation of Nrf2/Keap1 signaling during adipocyte differentiation

Kim, Chae Young; Kang, Bobin; Hong, Jungil; Hyun-Min, Choi

doi:10.1007/s10068-019-00672-y

Cited by 14 publications

(15 citation statements)

References 34 publications

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“…Nrf2 is a basic leucine zipper REDOX sensitive transcription factor that can regulate the REDOX state of cells when exposed to harmful stimuli [13]. Under basic condition, Nrf2 locates in the cytoplasm with its anchor inhibitors Kelch sample ECH associate protein 1 (Keap1) [14]. However, under oxidative stress, Nrf2 disintegrates from Keap1, relocates to the nucleus, and regulates downstream oxidation resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway

et al. 2020

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Background: Intracavernous injection of mesenchymal stem cells (MSCs) is a promising method for diabetic mellitus-induced erectile dysfunction (DMED), but short survival time of MSCs in cavernous is a fatal defect for therapy. This study investigated therapeutic efficiency and potential mechanism of probucol combined with MSCs. Methods: In vivo study, a total of forty-eight 10-week-old male Sprague-Dawley (SD) rats were used. Twelve rats received intraperitoneal injection of PBS as the sham group; the rest received intraperitoneal injection of 60 mg/kg streptozotocin to establish DM models. DM rats were randomly divided into three groups: received intracavernosal (IC) injection of either PBS (DM group), MSCs (M group), or administrated probucol after intracavernosal injection of MSCs (P + M group). Erectile function was assessed by electrical stimulation of the cavernous nerves with real-time intracavernous pressure measurement. After euthanasia, penile tissue was investigated for histologic examination and Western blotting. In in vitro experiment, H 2 O 2 was used to create oxidative stress environment to detect changes in cell viability. CCK8 was used to measure cell viability of MSCs treated with or without probucol. Intracellular ROS changes were detected by flow cytometry. Autophagy and apoptosis were detected by Western blotting and confocal microscopy.

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Section: Introductionmentioning

confidence: 99%

Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway

et al. 2020

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“…To determine whether the anti-mitotic effect was associated with alteration in the levels of adipogenic transcription factors, the effect of IAE on the activation of STAT3 and the expression of C/EBP-β was investigated in the MDI-stimulated 3T3-L1 preadipocytes during the early phase of differentiation. The activation of STAT3 and C/EBP-β mediates cell proliferation and activation of adipogenic transcription factors, thereby promoting MCE and adipogenesis [ 17 , 18 , 35 ]. Consistent with these findings, stimulation with MDI induced the phosphorylation of STAT3 and IAE attenuated the MDI-induced phosphorylation of STAT3 ( Figure 5 A).…”

Section: Resultsmentioning

confidence: 99%

Inula britannica Inhibits Adipogenesis of 3T3-L1 Preadipocytes via Modulation of Mitotic Clonal Expansion Involving ERK 1/2 and Akt Signaling Pathways

Kim

Bae

et al. 2020

Nutrients

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The flower of Inula britannica contains various phenolic compounds with prophylactic properties. This study aimed to determine the anti-adipogenic effect of an I. britannica flower aqueous extract (IAE) and its underlying mechanisms in the 3T3-L1 preadipocytes and to identify the phenolic compounds in the extract. Treatment with IAE inhibited the adipogenesis of 3T3-L1 preadipocytes by showing a dose-dependently suppressed intracellular lipid accumulation and significantly mitigated expression levels of lipogenesis- and adipogenesis-associated biomarkers including transcription factors. IAE exerted an anti-adipogenic effect through the modulation of the early phases of adipogenesis including mitotic clonal expansion (MCE). Treatment with IAE inhibited MCE by arresting the cell cycle at the G0/G1 phase and suppressing the activation of MCE-related transcription factors. Furthermore, IAE inhibited adipogenesis by regulating the extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Protocatechuic acid, chlorogenic acid, kaempferol-3-O-glucoside, and 6-methoxyluteolin, which are reported to exhibit anti-adipogenic properties, were detected in IAE. Therefore, modulation of early phases of adipogenesis, especially MCE, is a key mechanism underlying the anti-adipogenic activity of IAE. In summary, the anti-obesity effects of IAE can be attributed to its phenolic compounds, and hence, IAE can be used for the development of anti-obesity products.

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“…C/EBP‐α induces various adipogenesis‐associated genes in addition to PPAR‐γ. These transcription factors reciprocally encourage de novo lipogenesis and play a crucial role in maintaining the adipocytes in a differentiated state (Kim et al., 2020). It is widely known in numerous studies that the inhibition of these transcription factors plays key roles in suppressing adipocyte differentiation and advance of obesity.…”

Section: Discussionmentioning

confidence: 99%

Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3‐L1 preadipocytes

Kim

Bae

et al. 2021

J Food Biochem

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Herbs have been of interest to treat diseases, including obesity, owing to their various bioactive constituents that exhibit therapeutic and prophylactic properties. The present study examined the anti‐adipogenic effects and mechanisms of Chrysanthemum indicum aqueous extract (CAE) in 3T3‐L1 preadipocytes. CAE comprises 1,3‐dicaffeoylquinic acid, chlorogenic acid, kaempferol‐3‐O‐glucoside, caffeic acid, and apigenin, which were corresponded with previous reports. CAE inhibited the accumulation of lipid droplets and significantly alleviated the expression of lipogenesis‐ and adipogenesis‐associated biomarkers. Treatment with CAE inhibited the mitotic clonal expansion (MCE), corroborated by cell cycle arrest at the G0/G1 phase, and mitigated the expression of cell cycle progression‐associated proteins and in addition to phosphorylation of MCE‐promoting transcription factors. Moreover, CAE downregulated the activation of Akt and extracellular signal‐regulated kinase 1/2 signaling pathways. In summary, CAE facilitates adipogenic inhibition during the early phase of differentiation, especially MCE, and its phenolic compounds can contribute to its anti‐obesogenic properties. Practical applications Chrysanthemum indicum has been mainly used as traditional herbal tea and drinks. Chrysanthemum indicum aqueous extract (CAE) inhibits adipogenesis by suppressing mitotic clonal expansion during the early phase of differentiation in 3T3‐L1 preadipocytes. 1,3‐Dicaffeoylquinic acid, chlorogenic acid, kaempferol‐3‐O‐glucoside, caffeic acid, and apigenin were detected in CAE. Based on these findings, CAE can be used as nutraceutical agents for prevention and treatment of obesity.

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Parthenolide inhibits lipid accumulation via activation of Nrf2/Keap1 signaling during adipocyte differentiation

Cited by 14 publications

References 34 publications

Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway

Probucol enhances the therapeutic efficiency of mesenchymal stem cells in the treatment of erectile dysfunction in diabetic rats by prolonging their survival time via Nrf2 pathway

Inula britannica Inhibits Adipogenesis of 3T3-L1 Preadipocytes via Modulation of Mitotic Clonal Expansion Involving ERK 1/2 and Akt Signaling Pathways

Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3‐L1 preadipocytes

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