Parthenolide induces superoxide anion production by stimulating EGF receptor in MDA-MB-231 breast cancer cells

D’Anneo, Antonella; Carlisi, Daniela; Emanuele, Sonia; Buttitta, Giuseppina; Fiore, Riccardo Di; Vento, Renza; Tesoriere, Giovanni; Lauricella, Marianna

doi:10.3892/ijo.2013.2137

Cited by 18 publications

(13 citation statements)

References 35 publications

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“…It was observed that most of the cells were positive to DCF signal already at 1-3 h of treatment. Since this effect was suppressed by the addition of either apocynin or DPI, two effective inhibitors of NOX activity, we suggest that parthenolide, in line with previous findings (13,15), stimulated NOX activity, inducing production of O 2…”

Section: Discussionsupporting

confidence: 69%

“…In previous studies (13,15), we showed that parthenolide induces generation of ROS in MDA-MB-231 cells. The aim of the present study was to individuate the ROS produced under parthenolide treatment to specify the modalities of their production and the role exerted in the induction of cell death.…”

Section: Discussionmentioning

confidence: 99%

“…•-production in MDA-MB-231 cells as well (13,15), which could be responsible for the toxic effects caused by the drug. These effects consist in the depletion of thiol groups and glutathione, NF-κB inhibition, c-Jun N-terminal kinase (JNK) activation, dissipation of mitochondrial membrane potential (∆ψm) and necrotic effects (12,13).…”

Section: The Oxygen Radicals Involved In the Toxicity Induced By Partmentioning

confidence: 99%

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The oxygen radicals involved in the toxicity induced by parthenolide in MDA-MB-231 cells

et al. 2014

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Abstract.It has been shown that the sesquiterpene lactone parthenolide lowers the viability of MDA-MB-231 breast cancer cells, in correlation with oxidative stress. The present report examined the different radical species produced during parthenolide treatment and their possible role in the toxicity caused by the drug. Time course experiments showed that in the first phase of treatment (0-8 h), and in particular in the first 3 h, parthenolide induced dichlorofluorescein (DCF) signal in a large percentage of cells, while dihydroethidium (DHE) signal was not stimulated. Since the effect on DCF signal was suppressed by apocynin and diphenyleneiodonium (DPI), two inhibitors of NADPH oxidase (NOX), we suggest that parthenolide rapidly stimulated NOX activity with production of superoxide anion (O 2•-

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: The Oxygen Radicals Involved In the Toxicity Induced By Partmentioning

confidence: 99%

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The oxygen radicals involved in the toxicity induced by parthenolide in MDA-MB-231 cells

et al. 2014

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“…AG1478 is an EGFR selective inhibitor that principally blocks EGFR activation at Tyr1173 (28,29). In the present study, AG1478 increased AGS cell apoptosis, thus we speculate that the expression levels of EGFR may be associated with its activity.…”

Section: Discussionmentioning

confidence: 99%

High expression of EGFR predicts poor survival in patients with resected T3 stage gastric adenocarcinoma and promotes cancer cell survival

Wang

et al. 2017

Oncology Letters

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Epidermal growth factor receptor (EGFR) is an essential regulator and biomarker of several types of cancer. However, the association between its expression and prognosis in patients with resected T3 stage gastric adenocarcinoma (RT3-GA) remains to be determined. In total, 683 patients with resectable T3-GA who underwent surgery were retrospectively included in the present study, and their immunohistochemical data for EGFR expression were collected. The associations between the patients' clinicopathologic characteristics and EGFR immunohistochemistry data were analyzed by multiple statistical methods. Annexin V apoptosis and MTT cell viability assays were performed to explore the effect of EGFR on AGS gastric adenocarcinoma cell survival. EGFR expression levels were categorized into two groups: low (406 cases) and high (277 cases). High EGFR was demonstrated to be significantly associated with distant metastasis (P=0.043) and severely decreased median overall survival time (MOST) and recurrence-free survival time (MRFST). MOST and MRFST in the low EGFR group were 39 and 37 months, respectively; whereas in the high EGFR group these values were only 18 and 13 months (P=3.10×10−9 and P=6.74×10−8, respectively). Multivariate analysis confirmed that high EGFR expression levels were associated with poor survival, which was associated with significantly increased recurrence risk and ~2-fold elevation in mortality risk [hazard ratio (HR), 1.73; 95% confidence interval (CI), 1.43–2.10; P=2.37×10−8 and HR, 1.80; 95% CI, 1.50–2.17; P=3.80×10−10]. Inhibiting EGFR with AG1478 suppressed its effect on promoting AGS cell survival. These results suggest that high EGFR expression indicates poor survival in patients with RT3-GA, which may be correlated with EGFR promoting GA cell survival.

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“…The evaluation of estrogen, progesterone, and epidermal growth factor type 2 receptors (ER, PgR, and HER2, respectively) is widely used in clinical practice in order to obtain prognostic and predictive information [24]. Also, several other markers, such as B-cell lymphoma 2, androgen receptor, EGF, phosphatase and tensin homolog (PTEN), and phosphatidylinositol 3-kinase (PI3K), may have prognostic value in breast cancer.…”

Section: An Overview On Main Signaling Pathways Involved In Breast Camentioning

confidence: 99%

A headlight on liquid biopsies: a challenging tool for breast cancer management

et al. 2016

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Breast cancer is the most frequent carcinoma and second most common cause of cancer-related mortality in postmenopausal women. The acquisition of somatic mutations represents the main mechanism through which cancer cells overcome physiological cellular signaling pathways (e.g., PI3K/Akt/mTOR, PTEN, TP53). To date, diagnosis and metastasis monitoring is mainly carried out through tissue biopsy and/or re-biopsy, a very invasive procedure limited only to certain locations and not always feasible in clinical practice. In order to improve disease monitoring over time and to avoid painful procedure such as tissue biopsy, liquid biopsy may represent a new precious tool. Indeed, it represents a basin of “new generation” biomarkers that are spread into the bloodstream from both primary and metastatic sites. Moreover, elevated concentrations of circulating tumor DNA (ctDNA) as well as circulating tumor cells (CTCs) have been found in blood plasma of patients with various tumor types. Nowadays, several new approaches have been introduced for the detection and characterization of CTCs and ctDNA, allowing a real-time monitoring of tumor evolution. This review is focused on the clinical relevance of liquid biopsy in breast cancer and will provide an update concerning CTCs and ctDNA utility as a tool for breast cancer patient monitoring during the course of disease

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Parthenolide induces superoxide anion production by stimulating EGF receptor in MDA-MB-231 breast cancer cells

Cited by 18 publications

References 35 publications

The oxygen radicals involved in the toxicity induced by parthenolide in MDA-MB-231 cells

The oxygen radicals involved in the toxicity induced by parthenolide in MDA-MB-231 cells

High expression of EGFR predicts poor survival in patients with resected T3 stage gastric adenocarcinoma and promotes cancer cell survival

A headlight on liquid biopsies: a challenging tool for breast cancer management

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