Parthenogenetic Induction of Canine Oocytes by Electrical Stimulation and Ca‐EDTA

Abstract

In this study, we investigated parthenogenetic induction of canine oocytes by electrical stimulation following Ca-EDTA treatment. Oocyte maturation, parthenogenetic development, and cleavage rate in canine after various electrical stimulations (1.5, 1.8, 2.1 kV/cm) for 50 mus with single DC pulse following 1 mM Ca-EDTA treatment were investigated. In oocyte activated electrically at the voltage of 1.5 kV/cm after 1 mM Ca-EDTA treatment, the rate of pronucleus and two-cell was 4.1% and 2.7%, respectively. Although electrical stimulation could parthenogenetically induce immature oocyte to cleavage stage, degeneration rate in all experimental groups was more than 60%. This means that electrical stimulation after Ca-EDTA treatment could cause canine oocytes to be degenerated. However, two-cell in canine oocyte by parthenogenesis was for the first time induced. Therefore, we suggested that electrical stimulation for canine oocytes could induce parthenogenetically early embryonic cleavage. This result can be used as a basic data for parthenogenesis study in canine. Also, to perform more developed embryonic development, further study to parthenogenesis in canine need to be developed.

The canine oocyte: uncommon features of in vivo and in vitro maturation

“…10% of the preovulatory diameter). Such incompetence may contribute to the low efficiency of parthenogenesis following electrical stimulation or calcium treatment (Lee et al 2009;Kim et al 2010).…”

Partenogenetik Aktivasyonun Vitrifiye Köpek Oositleri Üzerine Etkisi

“…18,19 Partenogenetik aktivasyon ve mayotik başlangıç laboratuvar ortamında Ca-EDTA, ionomisin, siklohekzimid ve elektrik stimülasyonu ile köpek oositlerinde test edilmiştir. 5,19,20 Fakat in vitro maturasyon sonrasında vitrifikasyonla dondurulan köpek oositlerinin, partenogenetik aktivasyon testleri yapılmamıştır. Bu nedenle sunulan çalışmada, in vitro maturasyon sonrasında vitrifiye edilen köpek oositlerinin, kalsiyum ionofor ve siklohekzimid ile partenogenetik aktivasyonu sonrasında mayotik başlangıca etkisinin belirlenmesi amaçlanmıştır.…”

Insight into Sperm-less Conception of Virgin Mary from Reproductive Biology Perspective

“…Using a haploid number of chromosomes, male bees can have generative and haploid reproduction. Parthenogenetic reproduction can be induced in frogs, starfish, worms, and rabbits using certain chemicals like ionomycin as well as some alcohol derivatives used in culture media and intra-cytoplasmic calcium injections [14,15]. Parthenogenesis can be stimulated, though rarely, by leaving the oocytes in hypo-or hypertonic media, by hemolymph injection into the oocyte, by giving the oocyte a thermal shock, or by using media containing methanol and certain cations.…”