Parthenogenetic Embryo-Like Structures in the Human Ovarian Surface Epithelium Cell Culture in Postmenopausal Women with No Naturally Present Follicles and Oocytes

Virant‐Klun, Irma; Rožman, Primož; Cvjetičanin, Branko; Vrtačnik-Bokal, Eda; Novaković, Srdjan; Rülicke, Thomas; Dovč, Peter; Meden‐Vrtovec, Helena

doi:10.1089/scd.2007.0238

Cited by 140 publications

(165 citation statements)

References 36 publications

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“…Putative stem cells were found in the adult ovarian surface epithelium of different mammals, including humans [61,[78][79][80], in mouse ovarian stroma [21] and in the theca layer [29], which surrounds the developing follicle.…”

Section: Expression Of Stem Cell Markers In Granulosa Cells From Follmentioning

confidence: 99%

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Dzafic

Štimpfel

Virant‐Klun

2013

J Assist Reprod Genet

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The ovarian follicle represents the basic functional unit of the ovary and consists of an oocyte, which is surrounded by granulosa cells (GCs). GCs play an important role in the growth and development of the follicle. They are subject to increased attention since it has recently been shown that the subpopulation of GCs within the growing follicle possesses exceptionally plasticity showing stem cell characteristics. In assisted reproduction programs, oocytes are retrieved from patients together with GCs, which are currently discarded daily, but could be an interesting subject to be researched and potentially used in regenerative medicine in the future. Isolated GCs expressed stem cell markers such as OCT-4, NANOG and SOX-2, showed high telomerase activity, and were in vitro differentiated into other cell types, otherwise not present within ovarian follicles. Recently another phenomenon demonstrated in GCs is transdifferentiation, which could explain many ovarian pathological conditions. Possible applications in regenerative medicine are also given.

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Section: Expression Of Stem Cell Markers In Granulosa Cells From Follmentioning

confidence: 99%

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Dzafic

Štimpfel

Virant‐Klun

2013

J Assist Reprod Genet

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“…In follicular recruitment and sustainment, ovarian cell proliferation plays a primary role, with granulosa cell proliferation, differentiation, and subsequent senescence (aging) and apoptosis (death) to underpin oogenesis and the production of estrogen and progesterone. Recent studies have shown the presence of the cells in adult ovaries with germline stem cell properties of proliferative potential in the postnatal mammalian ovary (Virant-Klun et al 2009, Zou et al 2009). Implantation of a cell line established from the germline stem cells of adult mouse ovary into the ovaries of infertile mice reinitiates oogenesis and fertility (Zou et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The ovary is surrounded by a single layer of ovarian surface epithelium (OSE) that is a layer of cuboidal cells derived from mesoderm during embryonic development (Auersperg et al 2001). As a source of ovarian stem cells in humans (Virant-Klun et al 2009) and mice (Lee et al 2008), OSE undergoes hyperplasia in response to estrogen treatment to potentially lead to tumorigenesis (Laviolette et al 2010). Estrogen accelerates tumorigenesis initiated by the simian virus 40 (SV40) T antigens, resulting in an early onset and reduced overall survival time (Laviolette et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Telomerase in the ovary

Liu¹,

Li²

2010

Reproduction

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Telomerase, an enzyme complex that binds the chromosome ends (telomeres) and maintains telomere length and integrity, is present in germ cells, proliferative granulosa cells, germline stem cells, and neoplastic cells in the ovary, but it is absent in differentiated or aged cells. Activation of telomerase in the ovary underpins both benign and malignant cell proliferation in several compartments, including the germ cells, membrana granulosa, and the ovarian surface epithelium. The difference in telomerase operation between normal and abnormal cell proliferations may lie in the mechanisms of telomerase activation in a deregulated manner. Recent studies have implicated telomerase activity in ovarian cancer as well as oogenesis and fertility. Inhibition of telomerase and the shortening of telomeres are seen in occult ovarian insufficiency. Studies of how telomerase operates and regulates ovary development may provide insight into the development of both germ cells for ovarian reproductive function and neoplastic cells in ovarian cancer. The current review summarizes the roles of telomerase in the development of oocytes and proliferation of granulosa cells during folliculogenesis and in the process of tumorigenesis. It also describes the regulation of telomerase by estrogen in the ovary.

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“…Consequently, the BrdU/Mvh stained cells present in the OSE are not GSC, but most likely primordial oocytes during their physiologic extrusion process. Virant-Klun et al (16), described small round cells, above and below the postmenopausal OSE, which they considered to be GSC. However, they may correspond instead to the small immune cells previously described in this location by Motta et al (17).…”

Section: Are De Novo Oocytes Really Generated By the Ovarian Surface mentioning

confidence: 99%

There is no neo-oogenesis in the adult mammalian ovary

Gougeon¹,

Notarianni²

2011

J Turkish German Gynecol Assoc

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Parthenogenetic Embryo-Like Structures in the Human Ovarian Surface Epithelium Cell Culture in Postmenopausal Women with No Naturally Present Follicles and Oocytes

Cited by 140 publications

References 36 publications

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Telomerase in the ovary

There is no neo-oogenesis in the adult mammalian ovary

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