Parthenogenetic bovine embryos secrete type I interferon capable of stimulating ISG15 in luteal cell culture

Bridi, Alessandra; Bertolin, Kalyne; Rissi, Vitor Braga; Mujica, Lady Katerine Serrano; Glanzner, Werner Giehl; Macedo, Mariana Priotto de; Comim, Fábio Vasconcellos; Gonçalves, Paulo Bayard Dias; Antoniazzi, Alfredo Q.

doi:10.21451/1984-3143-ar2018-0095

Cited by 9 publications

(13 citation statements)

References 33 publications

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“…The main pathways changed by the embryo included type I interferon-response and genes associated to the prostaglandin metabolism. In agreement, recent in vitro studies showed that early bovine embryos were able to modulate gene expression of co-cultured endometrial 10–12 , oviductal 13 , luteal 14 and immune cells 10,15,16 . However, a critical unanswered question is whether the female tract has the ability to respond to pre-hatching embryo-derived signals beyond the transcription level, to change its transport and secretory functions and ultimately change the composition of the uterine microenvironment.…”

Section: Introductionsupporting

confidence: 83%

“…Accumulating evidence supports the idea that the early bovine embryo is more than a passive passenger through the maternal reproductive tract. Embryo-induced effects, at transcriptional level, on oviductal 13 , endometrial 9–12 , luteal 14 and immune 15,16 cells have been reported. In the present study, we further advanced the understanding of the embryo effects in early pregnancy.…”

Section: Discussionmentioning

confidence: 99%

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The pre-hatching bovine embryo transforms the uterine luminal metabolite composition in vivo

Sponchiado

Gonella-Diaza

Rocha

et al. 2019

Sci Rep

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In cattle, conceptus development after elongation relies on well-characterized, paracrine interactions with the hosting maternal reproductive tract. However, it was unrecognized previously that the pre-hatching, pre-implantation bovine embryo also engages in biochemical signalling with the maternal uterus. Our recent work showed that the embryo modified the endometrial transcriptome in vivo . Here, we hypothesized that the embryo modulates the biochemical composition of the uterine luminal fluid (ULF) in the most cranial portion of the uterine horn ipsilateral to the corpus luteum. Endometrial samples and ULF were collected post-mortem from sham-inseminated cows and from cows inseminated and detected pregnant 7 days after oestrus. We used quantitative mass spectrometry to demonstrate that the pre-hatching embryo changes ULF composition in vivo . Embryo-induced modulation included an increase in concentrations of lipoxygenase-derived metabolites [12(S)-HETE, 15(S)-HETE] and a decrease in the concentrations of amino acids (glycine), biogenic amines (sarcosine), acylcarnitines and phospholipids. The changed composition of the ULF could be due to secretion or depletion of specific molecules, executed by either the embryo or the endometrium, but initiated by signals coming from the embryo. This study provides the basis for further understanding embryo-initiated modulation of the uterine milieu. Early embryonic signalling may be necessary to guarantee optimal development and successful establishment of pregnancy in cattle.

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Section: Introductionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

The pre-hatching bovine embryo transforms the uterine luminal metabolite composition in vivo

Sponchiado

Gonella-Diaza

Rocha

et al. 2019

Sci Rep

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“…An important point in IFN-τ signaling is its capability to leave the uterine lumen and acts on distant tissues in an endocrine fashion. The endocrine effects of IFN-τ were confirmed by the expression of ISG in the liver (Meyerholz et al, 2015), luteal cells (Kiyma et al, 2016;Bridi et al, 2018) and immune cells in the bloodstream (Yankey et al, 2001;Hyungchul et al, 2006;Gifford et al, 2007;Kizaki et al, 2013;Pugliesi et al, 2014). Despite the fact that, no antiviral activity was detected in the main vessels (jugular) of the circulatory system.…”

Section: Autocrine Endocrine and Paracrine Actions Of Interferon-taumentioning

confidence: 97%

Conceptus-modulated innate immune function during early pregnancy in ruminants: a review

et al. 2021

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This review focuses on the innate immune events modulated by conceptus signaling during early pregnancy in ruminants. Interferon-tau (IFN-τ) plays a role in the recognition of pregnancy in ruminants, which involves more than the inhibition of luteolytic pulses of PGF2α to maintain corpus luteum function. For successful pregnancy establishment, the allogenic conceptus needs to prevent rejection by the female. Therefore, IFN-τ exerts paracrine and endocrine actions to regulate the innate immune system and prevent conceptus rejection. Additionally, other immune regulators work in parallel with IFN-τ, such as the pattern recognition receptors (PRR). These receptors are activated during viral and bacterial infections and in early pregnancy, but it remains unknown whether PPR expression and function are controlled by IFN-τ. Therefore, this review focuses on the main components of the innate immune response that are involved with early pregnancy and their importance to avoid conceptus rejection.

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“…In the uterus, IFNT acts in a paracrine way, decreasing estrogen and oxytocin receptor expression in the endometrium, which is an essential step to maintaining a viable corpus luteum and producing progesterone [33]. Besides antiluteolytic function, IFNT has endocrine effects, stimulating the expression of IFN-stimulated genes (ISGs) in the endometrium [34], in the corpus luteum [35][36][37], in white blood cells [38], and in the liver [39]. The effects of IFNT secreted by the bovine embryo in the maternal organism can be detected as early as day 7 of development [40,41].…”

Section: Embryo-maternal Interactions Mediated By Embryotropinsmentioning

confidence: 99%

Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

Bridi

Perecin

Silveira

2020

IJMS

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Embryo-maternal crosstalk is an important event that involves many biological processes, which must occur perfectly for pregnancy success. This complex communication starts from the zygote stage within the oviduct and continues in the uterus up to the end of pregnancy. Small extracellular vesicles (EVs) are part of this communication and carry bioactive molecules such as proteins, lipids, mRNA, and miRNA. Small EVs are present in the oviductal and uterine fluid and have important functions during fertilization and early embryonic development. Embryonic cells are able to uptake oviductal and endometrium-derived small EVs. Conversely, embryo-derived EVs might modulate oviductal and uterine function. In this review, our aim is to demonstrate the role of extracellular vesicles modulating embryo-maternal interactions during early pregnancy.2 of 15 luteotrophic and anti-luteolytic actions, guaranteeing the corpus luteum (CL)'s capability to secrete the progesterone necessary to establish and sustain pregnancy [15,16]. Additionally, different biological molecules are secreted by the embryos of several mammalian species during MRP as interferon-tau (IFNT) in ruminant [17], estradiol in pigs [18], and chorionic gonadotropin in humans [19]. However, recent reports indicate that the embryo is also able to secrete EVs [20][21][22], but their role in MRP is still elusive.Therefore, in mammals, the embryos and the female tract (oviduct and endometrium) are able to secrete extracellular vesicles during the pre-implantation period. Extracellular vesicles are cell-secreted vesicles that are classified according the their size, biogenesis, and secretion, as exosomes, microvesicles, and apoptotic bodies [23]. Several cellular types can secrete EVs including follicular [24,25], oviductal, and endometrial cells [13,21,26], as well as in vitro and in vivo produced embryos [20][21][22]27]. Extracellular vesicles carry bioactive molecule as miRNAs, mRNAs [28], proteins [29], and lipids [30], which modulate various reproductive processes such as ovarian follicular development, oocyte maturation, embryonic development, maternal-embryonic communication, and the establishment of mammalian gestation. There is experimental evidence that EVs participate in intercellular communication in essential reproductive events related to the establishment of pregnancy, such as cell proliferation, crosstalk between the maternal organism and conceptus, as well as during embryonic implantation [31]. In early stages of pregnancy, the communication between the conceptus and maternal organism is necessary and the evidence of the participation of EVs of maternal or embryonic origin is increasing [14].Herein, we will review the current knowledge on embryo-maternal interactions, with special emphasis on the roles of EVs during the early crosstalk between embryonic and maternal tissues.Int. J. Mol. Sci. 2020, 21, 1163 3 of 15 (reviewed by [47]). PGE2 secreted by equine viable embryos during early embryonic development is involved with the initial oviductal transpo...

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Parthenogenetic bovine embryos secrete type I interferon capable of stimulating ISG15 in luteal cell culture

Cited by 9 publications

References 33 publications

The pre-hatching bovine embryo transforms the uterine luminal metabolite composition in vivo

The pre-hatching bovine embryo transforms the uterine luminal metabolite composition in vivo

Conceptus-modulated innate immune function during early pregnancy in ruminants: a review

Extracellular Vesicles Mediated Early Embryo–Maternal Interactions

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