Parthenogenetic activation of sea urchin egg induces a cyclical variation of the cytoplasmic resistance to hexylene glycol-triton X-100 treatment

Coffe, Gérard; Rola, F.H.; Soyer, Marie-Odile; Pudles, J.

doi:10.1016/0014-4827(82)90008-8

Cited by 16 publications

(5 citation statements)

References 27 publications

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“…Moreover, a cyclic increase and decrease in the amount of cortical F-actin has been observed during early cleavage divisions, with a peak near the beginning of the cell cycle and a trough during cytokinesis [30]. In sea urchin eggs, weak bases such as procaine can stimulate pH-mediated actin turnover [67][68][69][70]. Moreover, ADF/cofilin (AC) proteins exhibit a pHdependent role in F-actin turnover [71,72] consisting of an alkalinity-associated increase in F-actin depolymerization activity, with a critical concentration around neutral pH [73], and a temporary concentration at the contractile ring during cytokinesis [74].…”

Section: Procaine Induces Cytokinesis In Equine Oocytesmentioning

confidence: 98%

Procaine Induces Cytokinesis in Horse Oocytes via a pH-Dependent Mechanism1

Leemans

Gadella

Stout

et al. 2015

Biology of Reproduction

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Coincubating equine gametes in the presence of procaine has been reported to facilitate in vitro fertilization, with cleavage rates exceeding 60%. We report that while procaine does trigger sperm hyperactivation, it independently induces cleavage of equine oocytes. First, we found that procaine (1-5 mM) did not facilitate stallion sperm penetration of equine oocytes but instead induced sperm-independent oocyte cytokinesis in the absence of the second polar body extrusion. Indeed, 56 ± 4% of oocytes cleaved within 2.5 days of exposure to 2.5 mM procaine regardless of sperm presence. However, the cleaved oocytes did not develop beyond 8 to 16 cells, and the daughter cells either lacked nuclei or contained aberrant, condensed DNA fragments. By contrast, intracytoplasmic sperm injection (ICSI) was followed by second polar body extrusion and formation of normal blastocysts. Moreover, neither the calcium oscillations detectable using fura-2 AM staining nor the cortical granule reaction visualized by LCA-FITC staining, after oocyte activation induced by ICSI or ionomycin treatment, were detected after exposing oocytes to 2.5 mM procaine. Instead, procaine initiated an ooplasmic alkalinization, detectable by BCECF-AM staining that was not observed after other treatments. This alkalinization was followed, after an additional 18 h of incubation, by cortical F-actin depolymerization, as demonstrated by reduced actin phalloidin-FITC staining intensity, that resembled preparation for cytokinesis in ICSI-fertilized zygotes. Overall, we conclude that procaine induces cytokinesis in equine oocytes accompanied by aberrant chromatin condensation and division; this explains why embryos produced after exposing equine oocytes to procaine fail to develop beyond the 8- to 16-cell stage.

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Section: Procaine Induces Cytokinesis In Equine Oocytesmentioning

confidence: 98%

Procaine Induces Cytokinesis in Horse Oocytes via a pH-Dependent Mechanism1

Leemans

Gadella

Stout

et al. 2015

Biology of Reproduction

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“…The device, still of very good quality, performed the expected services and was replaced by a new TEM 149 in 1982. Shortly after this, a first attempt was made 150 to establish an external team in cellular and molecular biology, that of Dr Julio Pudles, a biochemist from the University of Orsay who decided to make long stays in Banyuls, Prof. André Berkaloff, then head of porary establishment, our collaboration resulting in 156 several publications (Coffe et al, 1982). (Chatton and Soyer [-Gobillard] 1973).…”

Section: Article In Pressmentioning

confidence: 99%

Protistology and Cell Biology at the Marine Arago Laboratory of Banyuls-sur-Mer (1961–2000): Personal Recollections

Soyer‐Gobillard

2021

Protist

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“…Cyclic modifications have also been described after parthenogenetic activation of eggs. They mainly concern mechanical properties such as cytoplasmic resistance (Coffe et al, 1982), tubulin polymerization and depolymerization (Coffe et al, 1983), surface stiffness in sea urchin eggs (Yoneda et al, 1978), surface contraction waves in Xenopus eggs (Hara et al, 1980) or surface tension in starfish oocytes (Yamamoto & Yoneda, 1983).…”

Section: Cyclic Variations In Amino-acid Uptakementioning

confidence: 99%

Uptake of valine and alanine by a neutral amino-acid carrier in sea urchin eggs: cyclic variations in the early cleavage stage

Allemand¹,

Renzis²,

Maistre³

et al. 1985

J. Membrain Biol.

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Summary. The characteristics of valine and alanine uptake (respectively, preferential substrates of the L and A or ASC systems in mammalian cells) were studied in sea urchin eggs before and 40 min after fertilization. Substrate concentration dependence showed that in unfertilized eggs alanine is absorbed linearly up to an external concentration of 20 raM, whereas valine uptake presented a saturable kinetic with a Km of 6/xM. Competition experiments showed that valine is absorbed by a carriermediated transport resembling the L system. Fertilization develops a new Na-dependent system, resembling the ASC system which is specific for neutral amino acids but does not discriminate between them. This system is superimposed on that of the unfertilized egg. In fertilized eggs, amino-acid transport displayed cyclic variations which have been previously associated with cell cleavage. We have found that eggs prevented from cleavage by treatment with antimitotic undergo a sequence of periodic amino-acid uptake timed with the mitotic cycle of untreated eggs. In addition, artificially activated eggs (A23187) which failed to divide showed a time course of amino-acid uptake similar to that observed in fertilized eggs. Furthermore, these variations are independent of protein synthesis. These results suggest to us that a biological clock exists in the cytoplasm or cortex of sea urchin eggs, which may be involved in timing the cell cycle.

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Parthenogenetic activation of sea urchin egg induces a cyclical variation of the cytoplasmic resistance to hexylene glycol-triton X-100 treatment

Cited by 16 publications

References 27 publications

Procaine Induces Cytokinesis in Horse Oocytes via a pH-Dependent Mechanism1

Procaine Induces Cytokinesis in Horse Oocytes via a pH-Dependent Mechanism1

Protistology and Cell Biology at the Marine Arago Laboratory of Banyuls-sur-Mer (1961–2000): Personal Recollections

Uptake of valine and alanine by a neutral amino-acid carrier in sea urchin eggs: cyclic variations in the early cleavage stage

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