1983
DOI: 10.1016/0014-5793(83)80844-8
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Part of the 23S RNA located in the 11S RNA fragment is a constituent of the ribosomal peptidyltransferase centre

Abstract: Upon irradiation, 3‐[4‐benzoylphenyl]propionyl‐PhetRNA bound to the P‐site of poly(U)‐primed ribosomes is exclusively cross‐linked to 23S RNA. It is shown that the photoreaction only occurs with pyrimidine nucleotides. The site of the cross‐link is located within an 11S RNA fragment, which comprises the 1100 nucleotides at the 3′‐end of 23S RNA. The cross‐linked Phe tRNA derivative is still functionally active in peptide bond formation. The site labelled on the 11S fragment is therefore an integral part of the… Show more

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Cited by 16 publications
(13 citation statements)
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“…The sites of photoreaction can therefore be assigned to positions uridine-2584 and uridine-2585. The involvement of uridine residues is in agreement with our previous identification of the modified nucleotide from alkaline hydrolysis of photoaffinity-labeled 23S RNA (9). The photoaffinity-labeled uridine residues are located in the central loop of domain V of 23S rRNA (Fig.…”
Section: Discussionsupporting
confidence: 77%
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“…The sites of photoreaction can therefore be assigned to positions uridine-2584 and uridine-2585. The involvement of uridine residues is in agreement with our previous identification of the modified nucleotide from alkaline hydrolysis of photoaffinity-labeled 23S RNA (9). The photoaffinity-labeled uridine residues are located in the central loop of domain V of 23S rRNA (Fig.…”
Section: Discussionsupporting
confidence: 77%
“…1) as a probe of the peptidyl transferase region (7)(8)(9). The electron pair of the carbonyl group of the benzophenone moiety can be activated by UV irradiation at 320 nm to react with the C,-H bond of peptides as well as with nucleic acids (9,10).…”
mentioning
confidence: 99%
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“…In previous experiments, we developed a photo-affinity label for identifying components at or close to the peptidyl transferase region (Barta et al, 1975;Barta and Kuechler, 1983;Barta et al, 1984). Use was made of a benzophenone derivative [3-(4'-benzoylphenyl)propionic acid] covalently attached to the amino group of the Phe moiety of Phe-tRNA.…”
Section: Introductionmentioning
confidence: 99%
“…Already well documented is the role rRNA plays in mRNA initiation (2) and tRNA binding (3)(4)(5)(6). In addition, some rRNA sites have been implicated in the peptidyltransferase center (7), and subunit-subunit interactions (8)(9)(10).…”
mentioning
confidence: 99%