Paris Saponin II of Rhizoma Paridis – A novel inducer of apoptosis in human ovarian cancer cells

Xiao, Xue; Zou, Juan; Bui-Nguyen, Tri M.; Bai, Peng; Gao, Linbo; Liu, Jinsong; Liu, Shanling; Xiao, Jianzhong; Chen, Xinlian; Zhang, Xuemei; Wang, He

doi:10.5582/bst.2012.v6.4.201

Cited by 59 publications

(55 citation statements)

References 36 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While these results are consistent with reports in the literature on other saponins [38][39][40], the cancer cell cultures treated with glucolaxogenin in this study showed the presence of two cell populations, one with the characteristic morphology of apoptotic cells, and another with a morphology inconsistent with this type of cell death. In addition to apoptosis, the participation of different cell death processes is known.…”

Section: Discussionsupporting

confidence: 80%

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Sánchez-Sánchez

Escobar²,

Sandoval‐Ramírez

et al. 2015

Apoptosis

View full text Add to dashboard Cite

The antiproliferative and cytotoxic activity of glucolaxogenin and its ability to induce apoptosis and autophagy in cervical cancer cells are reported. We ascertained that glucolaxogenin exerts an inhibitory effect on the proliferation of HeLa, CaSki and ViBo cells in a dose-dependent manner. Analysis of DNA distribution in the cell-cycle phase of tumor cells treated with glucolaxogenin suggests that the anti-proliferative activity of this steroid is not always dependent on the cell cycle. Cytotoxic activity was evaluated by detection of the lactate dehydrogenase enzyme in supernatants from tumor cell cultures treated with the steroid. Glucolaxogenin exhibited null cytotoxic activity. With respect to the apoptotic activity, the generation of apoptotic bodies, the presence of active caspase-3 and annexin-V, as well as the DNA fragmentation observed in all tumor lines after treatment with glucolaxogenin suggests that this compound does indeed induce cell death by apoptosis. Also, a significantly increased presence of the LC3-II, LC3 and Lamp-1 proteins was evidenced with the ultrastructural existence of autophagic vacuoles in cells treated with this steroidal glycoside, indicating that glucolaxogenin also induces autophagic cell death. It is important to note that this compound showed no cytotoxic effect and did not affect the proliferative capacity of mononuclear cells obtained from normal human peripheral blood activated by phytohaemagglutinin. Thus, glucolaxogenin is a compound with anti-proliferative properties that induces programmed cell death in cancer cell lines, though it is selective with respect to normal lymphocytic cells. These findings indicate that this glycoside could have a selective action on tumor cells and, therefore, be worthy of consideration as a therapeutic candidate with anti-tumor potential.

show abstract

Section: Discussionsupporting

confidence: 80%

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Sánchez-Sánchez

Escobar²,

Sandoval‐Ramírez

et al. 2015

Apoptosis

View full text Add to dashboard Cite

show abstract

“…Anticarcinogenic activity was shown by a variety of steroidal saponins from different plants including Digitalis trojana (Kirmizibekmez et al 2014), Allium schoenoprasum (Timité et al 2013), Dioscorea zingiberensis (Tong et al 2012), Fagonia indica (Waheed et al 2012), Rhizoma Paridis (Xiao et al 2012), Solanum violaceum (Yen et al 2012), Agave sisalana (Chen et al 2011a, b), Anemarrhena asphodeloides (Kang et al 2011), Dioscorea bulbifera , Trigonella foenum-graecum (Kawabata et al 2011), Paris polyphylla (Zhu et al 2011), Raphia farinifera (Tapondjou et al 2015) etc.…”

Section: The Value Of Steroidal Saponinsmentioning

confidence: 99%

Recent advances in steroidal saponins biosynthesis and in vitro production

Upadhyay

Jeena

Shikha

et al. 2018

Planta

View full text Add to dashboard Cite

Main conclusion Steroidal saponins exhibited numerous pharmacological activities due to the modification of their backbone by different cytochrome P450s (P450) and UDP glycosyltransferases (UGTs). Plant-derived steroidal saponins are not sufficient for utilizing them for commercial purpose so in vitro production of saponin by tissue culture, root culture, embryo culture, etc, is necessary for its large-scale production.Saponin glycosides are the important class of plant secondary metabolites, which consists of either steroidal or terpenoidal backbone. Due to the existence of a wide range of medicinal properties, saponin glycosides are pharmacologically very important. This review is focused on important medicinal properties of steroidal saponin, its occurrence, and biosynthesis. In addition to this, some recently identified plants containing steroidal saponins in different parts were summarized. The high throughput transcriptome sequencing approach elaborates our understanding related to the secondary metabolic pathway and its regulation even in the absence of adequate genomic information of non-model plants. The aim of this review is to encapsulate the information related to applications of steroidal saponin and its biosynthetic enzymes specially P450s and UGTs that are involved at later stage modifications of saponin backbone. Lastly, we discussed the in vitro production of steroidal saponin as the plant-based production of saponin is time-consuming and yield a limited amount of saponins. A large amount of plant material has been used to increase the production of steroidal saponin by employing in vitro culture technique, which has received a lot of attention in past two decades and provides a way to conserve medicinal plants as well as to escape them for being endangered.

show abstract

“…Rhizoma paridis has been reported to exert numerous pharmacological effects, including anti-inflammatory, hemostatic and anti-cancer effects, and was shown to exhibit inhibitory effects on tumor growth in numerous studies using hepatic, gastric or nasopharyngeal carcinoma models (11)(12)(13)(14)(15)(16). Furthermore, Paris saponin II significantly inhibited tumor growth by 70% in the human SKOV3 ovarian cancer xenograft model (17), and Paris saponin H showed a marked cytotoxic activity on A549 cells with an IC 50 value of 1.53±0.08 µg/ml (18). Paris saponin D has been shown to overcome drug resistance in R-HepG2 cells, elicit programmed cell death via mitochondrial dysfunction, inhibit endothelial cell functions in vitro and inhibit angiogenesis in zebrafish embryos in vivo (19,20).…”

Section: Introductionmentioning

confidence: 97%

Paris saponin I induces apoptosis via increasing the Bax/Bcl-2 ratio and caspase-3 expression in gefitinib-resistant non-small cell lung cancer in vitro and in vivo

Jiang

Zhao

et al. 2014

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract. Polyphyllins, a major component of Rhizoma paridis, have been extensively used in non-small cell lung cancer (NSCLC). The aim of the present study was to evaluate the effects of Paris saponin I (PSI) on a panel of gefitinib-resistant NSCLC cell lines and its inhibition of tumor growth in a nude mouse model. The MTT assay was used to assess growth inhibition. The cell cycle was analyzed using flow cytometry and apoptosis was assessed using Annexin V/propidium iodide staining. The morphology of the apoptotic cells was determined by transmission electron microscopy. The protein expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) and caspase-3 were detected using western blot analysis. In addition, the glucose metabolism in tumor-bearing mice was evaluated using 18 F-fludeoxyglucose (FDG) micro-positron emission tomography imaging. The PSI-induced growth inhibition rate was observed to significantly increase in a time-and dose-dependent manner. Furthermore, PSI induced significant G2/M-phase arrest and apoptosis. The expression levels of Bcl-2 decreased, while those of Bax and caspase-3 increased following PSI treatment.18 F-FDG-uptake in the PSI treatment groups was significantly decreased compared with that in the control group in vivo. In conclusion, PSI is a potent antitumor agent that acts by inhibiting the proliferation of gefitinib-resistant cells, and has potential as a candidate for a natural drug for gefitinib-resistant therapy. PSI-induced apoptosis, which occurred via multiple pathways, including G2/M-phase arrest and upregulation of the Bax/Bcl-2 ratio and caspase-3 expression, ultimately led to cell death and tumor inhibition.

show abstract

Paris Saponin II of Rhizoma Paridis – A novel inducer of apoptosis in human ovarian cancer cells

Cited by 59 publications

References 36 publications

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Recent advances in steroidal saponins biosynthesis and in vitro production

Paris saponin I induces apoptosis via increasing the Bax/Bcl-2 ratio and caspase-3 expression in gefitinib-resistant non-small cell lung cancer in vitro and in vivo

Contact Info

Product

Resources

About