Parhyale hawaiensis as a promising alternative organism for monitoring acute toxicity of sediments under the influence of submarine outfalls

Vacchi, Francine Inforçato; Santos, Amanda dos; Artal, Mariana Coletty; Magalhães, Gabriel Rampazzo; Vendemiatti, Josiane Aparecida de Souza; Umbuzeiro, Gisela de Aragão

doi:10.1016/j.marpolbul.2019.110658

Cited by 10 publications

(2 citation statements)

References 24 publications

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“…Because of the increasing interest in the use of the marine amphipod Parhyale hawaiensis in ecotoxicology (Artal et al, 2018, 2019; Botelho et al, 2022; dos Santos, Botelho, et al, 2022; Dos Santos, Vannuci‐Silva, et al, 2022; Vacchi et al, 2019; Vannuci‐Silva et al, 2018, 2019) and in other sciences (Paris et al, 2022; Rehm et al, 2009), we here describe their hemocyte characteristics and identify the various cell types and their proportions, as well as their ability to phagocytize bacteria.…”

Section: Introductionmentioning

confidence: 99%

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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Hemocytes are circulating blood cells that play a crucial function in amphipods and other crustacean immune systems. The hemocytes of the marine tropical amphipod Parhyale hawaiensis have been used for the evaluation of DNA damage and micronuclei, but they have not been characterized in the scientific literature.The aim of this study was to describe the hemolymph cells of P. hawaiensis and study their phagocytotic activity. Basic dyes were used to differentiate the cell types and the presence of lipids. The total hemocyte counts (THCs) and the proportion and sizes of the hemocyte types were determined. Hemolymph was exposed to Escherichia coli for verification of the presence of phagocytosis. Three cell types, all containing lipids, were identified in P. hawaiensis: granulocytes (oval shape, 13.4 Â 7.6 μm), semi-granulocytes (oval shape, 14.1 Â 7.2 μm), and hyalinocytes (round shape, 9.6 Â 7.2 μm). Those three cell types were found in different percentages in males (64.8%, 31.1%, and 4.2%) and females (70.1%, 28.2%, and 1.7%). THCs for males were 9007 ± 3800 cells per individual and 4695 ± 1892 cells per individual for females. The cells of E. coli were phagocytized by the hemocytes. Our findings increased the knowledge of hemocytes in P. hawaiensis and is a step forward in using hemocyte-based immune responses as an endpoint in ecotoxicology.

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Section: Introductionmentioning

confidence: 99%

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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“…Because of their purpose of use, synthetic colorants are chemically very stable and can persist for long periods when they enter the environment. Even low concentrations of dyes in water systems can reduce the penetration of sunlight and, as a result, inhibit photosynthesis, causing oxygen deprivation and toxic effects to aquatic organisms from many trophic levels [3][4][5][6]. It has also been studied that certain azo dyes-the largest class of synthetic colorants-pose occupational health risks, such as allergic sensitization and a higher prevalence of some cancers [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Waterless Dyeing and In Vitro Toxicological Properties of Biocolorants from Cortinarius sanguineus

Herrala

Yli-Öyrä

Albuquerque

et al. 2022

JoF

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As a part of an ongoing interest in identifying environmentally friendly alternatives to synthetic dyes and in using liquid CO2 as a waterless medium for applying the resulting colorants to textiles, our attention turned to yellow-to-red biocolorants produced by Cortinarius sanguineus fungus. The three principal target anthraquinone colorants (emodin, dermocybin, and dermorubin) were isolated from the fungal bodies using a liquid–liquid separation method and characterized using 700 MHz NMR and high-resolution mass spectral analyses. Following structure confirmations, the three colorants were examined for dyeing synthetic polyester (PET) textile fibers in supercritical CO2. We found that all three biocolorants were suitable for dyeing PET fibers using this technology, and our attention then turned to determining their toxicological properties. As emodin has shown mutagenic potential in previous studies, we concentrated our present toxicity studies on dermocybin and dermorubin. Both colorants were non-mutagenic, presented low cellular toxicity, and did not induce skin sensitization. Taken together, our results indicate that dermocybin and dermorubin possess the technical and toxicological properties needed for consideration as synthetic dye alternatives under conditions that are free of wastewater production.

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Antenna regeneration as an ecotoxicological endpoint in a marine amphipod: a proof of concept using dimethyl sulfoxide and diflubenzuron

et al. 2021

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Parhyale hawaiensis as a promising alternative organism for monitoring acute toxicity of sediments under the influence of submarine outfalls

Cited by 10 publications

References 24 publications

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Waterless Dyeing and In Vitro Toxicological Properties of Biocolorants from Cortinarius sanguineus

Antenna regeneration as an ecotoxicological endpoint in a marine amphipod: a proof of concept using dimethyl sulfoxide and diflubenzuron

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