Parenteral Injections

Abstract

This unit describes the techniques for the following routes of injection for mice, rats, hamsters and rabbits: intramuscular, intradermal, subcutaneous, intravenous, intraperitoneal, footpad, and intrathymic. Guidelines are also given regarding injection volumes and temperatures, and the use of proper restraints.

Generation of Gene Knockout Mice by ES Cell Microinjection

“…C57Bl/6NCr females or equivalent strain (3 to 4 weeks of age; ten are needed per injection day 50 IU/ml pregnant mare's serum gonadotrophin (PMSG; see recipe) 50 IU/ml human chorionic gonadotrophin (HCG; see recipe) 10 C57Bl/6NCr males (7 weeks to 10 months of age) 1-cc tuberculin syringes Additional reagents and equipment for injection of mice (Donovan and Brown, 2006b) 1. At a time point 6 days before the planned day of blastocyst injections, intraperitoneally inject 0.1 ml of 50 IU/ml PMSG at 1300 hr into each C57BL/6NCRr female mouse.…”

“…Intraperitoneal injection is described in Donovan and Brown (2006b). For this injection and that in the following step, use a 1-cc tuberculin syringe and a 30-G, 1/2-in.…”

“…25-G, 5/8-in. needles 1-cc tuberculin syringes Sterile cotton swabs 37 • C slide warmer Additional reagents and equipment for injection of the mouse (Donovan and Brown, 2006b) Load transfer pipets with injected blastocysts 1. Take an already-made transfer pipet and load mineral oil to the hub of the transfer pipet by mouth pipetting (see Fig.…”

“…Surgically transfer embryos 10. Swab the abdomen of an E2.5-day pseudopregnant mouse with an ethanol pad and anesthetize the mouse by intraperitoneal injection (Donovan and Brown, 2006b) of 0.015 to 0.017 ml/g body weight of 2.5% Avertin.…”

Retinal Gene Delivery by rAAV and DNA Electroporation

“…Intravitreal injections tend to be easier, since the targeting area is larger. Injections can be performed with glass needles or metal needles (Hamilton), and in both cases the Materials Naturally delivered newborn mouse pups (0-to 1-day old) of any strain Buprenorphine Virus: rAAV can be produced in-house (UNIT 14D.1), or obtained through a local vector core or via a commercial manufacturer 0.05% (w/v) Fast Green solution (Sigma,or Additional reagents and equipment for injection of mice (Donovan and Brown, 2006a) 1. Remove naturally delivered mouse pups from the mother (all at once) and inject each pup subcutaneously (Donovan and Brown, 2006a) with buprenorphine (0.1 mg/kg) using an insulin injection needle.…”