Parental exposure to azoxystrobin causes developmental effects and disrupts gene expression in F1 embryonic zebrafish (Danio rerio)

Cao, Fangjie; Li, Hui; Zhao, Feng; Wu, Peizhuo; Qian, Le; Huang, Lan; Pang, Sen; Martyniuk, Christopher J.; Qiu, Lihong

doi:10.1016/j.scitotenv.2018.07.331

Cited by 33 publications

(19 citation statements)

References 37 publications

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“…It is important to notice that the control group had no increase of lethality rates, and only two larvae had scoliosis (72 hpf). Other compounds were reported to induce developmental toxicity in the offspring after parental treatment, such as bisphenol S [70], fusaric acid [71], selenomethionine [72], ZnO nanoparticles [73], and azoxystrobin [74].…”

Section: Discussionmentioning

confidence: 99%

Acmella oleracea(L) R. K. Jansen Reproductive Toxicity in Zebrafish: AnIn VivoandIn SilicoAssessment

Souza

Pereira

Viana

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The plant species Acmella oleracea L. is used in the north of Brazil for the treatment of a range of illnesses, such as tuberculosis, flu, cough, and rheumatism and as an anti-inflammatory agent; besides, hydroethanolic formulations with this species are popularly used as a female aphrodisiac agent. However, currently, there are no studies performed evaluating its effect on embryonic development. Hence, this research aimed to evaluate the effects of the hydroethanolic extract of A. oleracea (EHFAo) on the reproductive performance (parental) and embryonic development (F1 generation) of zebrafish, at concentrations of 50, 100, and 200 μg/L. Histopathology of parental gonads after 21 days of exposure to EHFAo reveals few alterations in the ovaries and testes, not impairing the reproduction; an increase of eggs deposition was observed in animals treated with EHFAo at the highest concentrations. Nevertheless, concerning the embryonic development of F1, teratogenic effects were observed including tail deformation, cardiac and yolk edema, scoliosis, and growth retardation; these alterations were more prominent in the groups born from progenitors exposed to the highest concentrations (100 and 200 μg/L.); but only the occurrence of yolk and cardiac edema had a statistically significant difference when compared to the control group. The chromatographic analysis shows that spilanthol (affinin) was the primary compound found in the EHFAo. Hence, in silico assessment was performed to evaluate the pharmacokinetic and toxicological properties of this molecule and 37 metabolites derived from it. Overall, our data show that the treatment caused no detrimental changes in progenitors regarding their gonads or fertility but caused some potentially teratogenic activity in embryos, which may be due to the action of spilanthol’s metabolites M3, M6, M7, M8, M16, M28, and M31.

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Section: Discussionmentioning

confidence: 99%

Acmella oleracea(L) R. K. Jansen Reproductive Toxicity in Zebrafish: AnIn VivoandIn SilicoAssessment

Souza

Pereira

Viana

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Several studies have already reported the transfer of toxicity from the parental generation to F1 generation of various substances such as glyphosate, 93 bisphenol S, 98 fusaric acid, 99 selenomethionine, 100 nanoparticles of ZnO, 101 and azoxystrobin. 102…”

Section: Discussionmentioning

confidence: 99%

“…108 The volume is therefore often used in QSAR studies to model molecular properties and biological activity within the range. 102,109 As the number of rotating bonds increases, the molecule becomes more flexible and more adaptable for efficient interaction with a particular binding pocket. 28 The values are shown in Table 3.…”

Section: Discussionmentioning

confidence: 99%

Reproductive toxicity of the hydroethanolic extract of the flowers ofAcmella oleraceaand spilanthol in zebrafish: In vivo and in silico evaluation

et al. 2019

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Hydroethanolic preparations of Acmella oleracea is used in the north of Brazil as a female aphrodisiac. Thus, the objective of this study was to evaluate the action of the hydroethanolic extract of Acmella oleracea (EHFAo) flowers (21.873 and 44.457 mg/kg) and spilanthol (3 mg/kg) administered orally on reproductive performance and effects on the embryonic development of zebrafish F1 generation. It was observed that in the groups in which males and females received EHFAo and spilanthol, the spawning was interrupted, whereas in the groups in which only the females were treated, spawning occurred during the 21 days. Thus, in the histopathological evaluation of the gonads, it was possible to observe that the percentage of mature cells in the spermatozoa and females was significantly reduced. Only the embryo groups in which parental generation was treated with EHFAo showed lethal and teratogenic effects. On the other hand, the parental groups treated with the spilanthol presented only the lethality. Spilanthol and some metabolites showed good oral availability and important toxicological properties. Thus, it is suggested that the treatment of parental generation of zebrafish with EHFAo and spilanthol caused severe changes in the gonads and on fertility. However, on the embryo, the most striking effects in the development were recorded in the groups in which the parental generation was treated with the EHFAo, while the spilanthol influenced the lethality of the embryos.

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“…Tebuconazole has been shown to be maternally transferred to zebrafish embryos resulting in decreased heart rate in developing larvae [51]. Another fungicide, azoxystrobin, was shown to alter mortality and development of zebrafish embryos when exposed to adult zebrafish [52].…”

Section: Contaminantsmentioning

confidence: 99%

Dietary Contaminants and Their Effects on Zebrafish Embryos

Tye

Masino

2019

Toxics

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Dietary contaminants are often an over-looked factor in the health of zebrafish. Typically, water is considered to be the source for most contaminants, especially within an aquatic environment. For this reason, source water for zebrafish recirculating systems is highly regulated and monitored daily. Most facilities use reverse osmosis or de-ionized water filtration systems to purify incoming water to ensure that contaminants, as well as pathogens, do not enter their zebrafish housing units. However, diets are rarely tested for contaminants and, in the case of manufactured zebrafish feeds, since the product is marketed for aquaculture or aquarium use it is assumed that the feed is acceptable for animals used for research. The following provides examples as to how contaminants could lead to negative effects on development and behavior of developing zebrafish.

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Parental exposure to azoxystrobin causes developmental effects and disrupts gene expression in F1 embryonic zebrafish (Danio rerio)

Cited by 33 publications

References 37 publications

Acmella oleracea(L) R. K. Jansen Reproductive Toxicity in Zebrafish: AnIn VivoandIn SilicoAssessment

Acmella oleracea(L) R. K. Jansen Reproductive Toxicity in Zebrafish: AnIn VivoandIn SilicoAssessment

Reproductive toxicity of the hydroethanolic extract of the flowers ofAcmella oleraceaand spilanthol in zebrafish: In vivo and in silico evaluation

Dietary Contaminants and Their Effects on Zebrafish Embryos

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