Parental contribution in a cultivated stock for the spotted rose snapper Lutjanus guttatus (Steindachner, 1869) estimated by newly developed microsatellite markers

Abstract

The spotted rose snapper Lutjanus guttatus is a fishery relevant species from the eastern Pacific Ocean, with aquaculture potential. Species-specific genetic markers are needed for the genetic characterization of wild and cultivated populations to help management strategies. Eighteen hypervariable microsatellites were developed by Next Generation Sequencing and characterized in a wild population sample. Genetic diversity was high (observed heterozygosity = 0.88 ± 0.050; the number of alleles per locus = 13.4 ± 1.3) and few loci departed from the Hardy-Weinberg Equilibrium, leaving 14 loci potentially suitable for population genetic studies. A reduced panel of five loci was tested in a cultivated stock to determine the parentage of progeny (embryonated eggs; n = 413), to estimate the temporal contribution of each parental broodstock. The above resulted in the successful assignment of 95.6% of the progeny to its parental couple, representing 17 out of the 24 possible families. Two of the four females produced most of those progeny (97.3%). These females, which reproduced throughout the season, did not spawn on consecutive days. The contribution of males was evenly distributed during the season and occurred on successive days. Some microsatellites can be used in other lutjanids (L. peru, L. argentiventris, and Hoplopagrus guentherii).

Spawning dynamics and egg production characteristics of captive Seriola dorsalis assessed using parentage analyses

“…This spawning dynamic is characterized by multiple males courting a single female to initiate spawning and synchronize the release of gametes (Gonçalves & Oliveira, 2010). This spawning dynamic has also been reported in other pelagic species (Gruenthal et al, 2014; Hutchings et al, 1999; Magnusson & Prescott, 1996; Margulies et al, 2007; Perez‐Enriquez et al, 2020; Smith, 1986). In other species, such as the yellowfin tuna ( Thunnus albacares ), a spawning event consists of several small‐group spawning sessions over a short period; in this spawning dynamic, a greater total number of females contribute to the spawning event on a given day (Margulies et al, 2007).…”

“…Genetic markers, such as microsatellites, have been successfully applied to a number of aquaculture species to evaluate parentage and pedigree relationships (Gold et al, 2010; Gruenthal & Drawbridge, 2012; Norris et al, 2000; Perez‐Enriquez et al, 2020; Porta et al, 2009; Whatmore et al, 2013). In aquaculture, these analyses are indispensable in developing and evaluating breeding programmes (Fessehaye et al, 2006), minimizing inbreeding effects (Sekino et al, 2003; Vandeputte & Haffray, 2014) and preventing genetic diversity loss due to reproductive variance (Boudry et al, 2002; Rodriguez‐Barreto et al, 2013).…”

Drivers for genetic structure at different geographic scales for Pacific red snapper (Lutjanus peru) and yellow snapper (Lutjanus argentiventris) in the tropical eastern Pacific

“…A total of 14 (Supporting Information SUPPORTING INFORMATION SI 3) and 12 (Supporting Information SUPPORTING INFORMATION SI 4) microsatellite loci were amplified for L. peru (Paz‐García et al ., 2016, Perez‐Enriquez et al ., 2020) and L. argentiventris (Perez‐Enriquez et al ., 2020, Reguera‐Rouzaud et al ., 2020), respectively. For the PCR, a touchdown protocol (Munguía‐Vega et al ., 2013) was used with a fluorescent label M13 primer (Schuelke, 2000) in a thermal cycler (Applied Biosystems MiniAmp Plus).…”

Genetic Variability of Mass-Selected and Wild Populations of Yellow Drum (Nibea albiflora) Revealed Using Microsatellites

“…This may be because the populations sampled in the previous study were wild populations, and the sources of the five populations in this study were more diverse, including wild, artificially propagated, and breeding populations; with the passage of more generations, more random genetic drift has occurred. This may be because, when the basic population is initially established, the gynogenetic individuals maintained a strain genetic relationship with the parent generation [42,43]. Population differentiation is primarily influenced by selection, genetic drift, gene flow, and their interrelationships.…”