Parental attitude towards their children with mental retardation

Yadav, V. N.; Bhan, Chander; Babita, .; Archana, .

doi:10.1037/e682942012-102

Cited by 2 publications

(4 citation statements)

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“…34,35 The presence of polar amino acid residues, like Thr 125 , Asn 67 , and Glu 68 , near the metal-binding site and surface-exposed Tyr 64 in Tma12 support it as a chitinbinding LPMO. 25,36 It is worth mentioning here that His 25 in 25 is considered as His 1 because it is the first amino acid residue of mature Tma12. No digestion of β-chitin nanofibers in the absence of ascorbic acid shows that Tma12 also depends upon a reductant for its enzymatic activity, as reported previously for other LPMOs.…”

Section: Resultsmentioning

confidence: 99%

“…It appears that Phe 69 (another surfaceexposed aromatic residue) in Tma12 present near the substrate binding surface might have a role in substrate binding. 25 In contrast, the catalytic assay revealed a remarkable reduction of LPMO activity in Tma12 apo (Figure 5B). On the other hand, Tma12 showed a marginal reduction in activity after DEPC modification.…”

Section: Lc−ms/ms Analysis Of Depc-labeled Tma12mentioning

confidence: 88%

“…24 Later on, X-ray crystallography reveals that Tma12 inherits structural similarities with the AA10 LPMO family. 25 The Amplex red assay suggested LPMO-like activity in Tma12. Although the enzymatic activity of Tma12 on carbohydrates has been briefly worked out, we are still not sure whether Tma12 is an active LPMO, and if so, its enzymatic activity is associated with insecticidal activity.…”

Section: Introductionmentioning

confidence: 99%

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Lytic Polysaccharide Monooxygenase Activity of Tma12 Is Critical for Its Toxicity to Whitefly

Singh,

Singh

et al. 2023

J. Agric. Food Chem.

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Lytic polysaccharide monooxygenases (LPMOs) are powerful redox enzymes that transform complex carbohydrates through oxidation and make them suitable for saccharification by canonical hydrolases. Due to this property, LPMOs are considered to be a valuable component of enzymatic consortia for industrial biorefineries. Tma12 is a fern entomotoxic protein that kills whitefly and has structural similarities with chitinolytic LPMO. However, its enzymatic activity is poorly understood. Studying the role of the LPMO-like activity in the insecticidal function of Tma12 can be of considerable importance. Our results show that Tma12 preferentially binds and digests β-chitin. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis shows that the digestion of chitin produces chitin oligosaccharides of various lengths (DP2–DP7). The Michaelis constant (k m) and catalytic constant (k cat) for hydrocoerulignone are 0.022 mM and 0.044 s–1, respectively. The attenuation of catalytic activity through diethylpyrocarbonate modification abolishes the insecticidal activity of the protein. Our findings reveal that (a) Tma12 is an active LPMO and (b) LPMO activity is indispensable for its function as a bioinsecticide.

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Section: Resultsmentioning

confidence: 99%

Section: Lc−ms/ms Analysis Of Depc-labeled Tma12mentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Lytic Polysaccharide Monooxygenase Activity of Tma12 Is Critical for Its Toxicity to Whitefly

Singh,

Singh

et al. 2023

J. Agric. Food Chem.

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“…Capmatinib inhibits c-Met-mediated phosphorylation of proteins downstream signaling and the propagation and survival of tumor c-Met-dependent cells. 5 It does this by inhibiting the phosphorylation of both wild-type and mutant variants of c-Met, which is triggered by the binding of its endogenous ligand, hepatocyte growth factor. [6][7][8] It is chemically named as 2-fluoro-N-methyl-4-{7-[(quinolin-6-yl) methyl] imidazo [1,2-b] [1,2,4] triazin-2-yl} benzamide, with chemical formula and weight of C 23 H 17 FN 6 O and 412.428 g•mol −1 (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating an LC-MS/MS Method Development and Validation for the Quantification of Capmatinib in Human Plasma

Panigrahi

2023

IJPQA

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A linear and accurate liquid chromatographic tandem mass spectrometric (LC-MS/MS) technique for the quantitation of capmatinib in plasma was developed and subjected for validation. Canagliflogin was employed as an internal standard (IS). Extraction of plasma was executed utilizing 5 mL of ethyl acetate solvent. Analysis was performed Zorbax ODS (50 mm × 2.1 mm x 3 μ) stationary phase at room conditions and a movable solvent composition of 0.1% HCOOH, acetonitrile and methyl alcohol (15:50:35). The flowing rate of the movable phase was 0.4 mL/min. Drug and IS were identified in positive mode of ionization with electrospray mode to get the mass transitions of (m/z): Capmatinib, 413.15/386.14 and canagliflogin (IS), 445.15/267.12. The correlation between capmatinib concentrations and their respective peak proportions to canagliflogin was a straight line over 0.2 to 3200 ng/mL concentration levels. Intra day and inter-day precisions were ≤5.18% for capmatinib. Inter and intra day bias were within the range of −4.17 to 4.53%. The mean measured extraction recovery of capmatinib was 99.23%. Recovery of IS was 98.31%. Capmatinib was subjected for long-term, freeze thaw, bench top, short-term stability, auto-sampler, dry extract, and stock solution stability at low QC and high QC levels and it was stable at all these conditions. The established technique can be utilized to regularly quantitfy of capmatinib in plasma samples in industries, forensic labs, and clinical research organizations.

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Parental attitude towards their children with mental retardation

Cited by 2 publications

References 0 publications

Lytic Polysaccharide Monooxygenase Activity of Tma12 Is Critical for Its Toxicity to Whitefly

Lytic Polysaccharide Monooxygenase Activity of Tma12 Is Critical for Its Toxicity to Whitefly

Stability Indicating an LC-MS/MS Method Development and Validation for the Quantification of Capmatinib in Human Plasma

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