Paratuberculosis in cattle: A comparison of three serologic tests with results of fecal culture

Colgrove, Gary S.; Thoen, Charles O.; Blackburn, Billie O.; Murphy, Cain

doi:10.1016/0378-1135(89)90083-7

Cited by 55 publications

(31 citation statements)

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“…As reported for treated animals in the accompanying report, ELISA values less than 2.5 had no predictive value for detectable fecal shedding, whereas animals with higher values were more likely to be detectable shedders. AGID positive animals were assumed to be more heavily infected than AGID negative animals since (a) AGID positive sera was always detected at the same time or subsequent to a positive ELISA; no AGID positive animal was simultaneously ELISA negative, [31][32][33][34] (b) the appearance of AGID positive serum and detected fecal shedding closely paralleled one another, 31,32 and (c) loss of detectable AGID always occurred prior to any reduction in ELISA values.…”

Section: Methodsmentioning

confidence: 99%

Assessment ofDietziasubsp.C79793-74for treatment of cattle with evidence of paratuberculosis

Click

Kampen

2010

Virulence

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Section: Methodsmentioning

confidence: 99%

Assessment ofDietziasubsp.C79793-74for treatment of cattle with evidence of paratuberculosis

Click

Kampen

2010

Virulence

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“…Also, previously developed PCR-based approaches (e.g., IS900) have been shown to lack specificity (9). This result is, in part, due to the high levels of genetic similarity of Map with other mycobacteria, in particular, Mycobacterium avium (Mav) (10). Previous studies from our laboratories and elsewhere show Ͼ95% nucleotide sequence similarity between many strains of Map and Mav (11)(12)(13).…”

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confidence: 97%

The complete genome sequence ofMycobacterium aviumsubspeciesparatuberculosis

Bannantine

Zhang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

407

356

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We describe here the complete genome sequence of a common clone of Mycobacterium avium subspecies paratuberculosis (Map) strain K-10, the causative agent of Johne's disease in cattle and other ruminants. The K-10 genome is a single circular chromosome of 4,829,781 base pairs and encodes 4,350 predicted ORFs, 45 tRNAs, and one rRNA operon. In silico analysis identified >3,000 genes with homologs to the human pathogen, M. tuberculosis (Mtb), and 161 unique genomic regions that encode 39 previously unknown Map genes. Analysis of nucleotide substitution rates with Mtb homologs suggest overall strong selection for a vast majority of these shared mycobacterial genes, with only 68 ORFs with a synonymous to nonsynonymous substitution ratio of >2. Comparative sequence analysis reveals several noteworthy features of the K-10 genome including: a relative paucity of the PE͞PPE family of sequences that are implicated as virulence factors and known to be immunostimulatory during Mtb infection; truncation in the EntE domain of a salicyl-AMP ligase (MbtA), the first gene in the mycobactin biosynthesis gene cluster, providing a possible explanation for mycobactin dependence of Map; and Map-specific sequences that are likely to serve as potential targets for sensitive and specific molecular and immunologic diagnostic tests. Taken together, the availability of the complete genome sequence offers a foundation for the study of the genetic basis for virulence and physiology in Map and enables the development of new generations of diagnostic tests for bovine Johne's disease.

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“…Serological assays including agar gel immunodiffusion, enzyme-linked immunosorbent assay, and complement fixation tests have been developed to detect antibodies against M. avium subsp. paratuberculosis in animal sera (6,8). Depending on the antigens used, these assays can yield false-positive results and may not consistently detect infected animals in the early stages of disease (13).…”

mentioning

confidence: 99%

Characterization of Novel Coding Sequences Specific to Mycobacterium avium subsp. paratuberculosis : Implications for Diagnosis of Johne's Disease

et al. 2004

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Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's Disease, an economically important intestinal ailment of ruminants. Due to the considerable genetic and serologic cross-reactivity with closely related and ubiquitous members of the M. avium complex, a species-specific method for the serological diagnosis of Johne's disease is unavailable. Computational and PCR-based analysis of the complete genome sequence of M. avium subsp. paratuberculosis led to the identification of 13 open reading frames with no identifiable homologs. One of these sequences is a putative insertion element present in six copies on the M. avium subsp. paratuberculosis genome. These novel M. avium subsp. paratuberculosis genes were cloned into Escherichia coli expression vectors, and nine were successfully expressed as recombinant fusion proteins. Five of these proteins were purified in sufficient amounts to allow immunoblot analyses of their reactivity with sera from naturally infected cattle as well as mice and rabbits exposed to M. avium subsp. paratuberculosis. Fusion proteins representing MAP0862, MAP3732c, and MAP2963c were recognized by nearly all of the sera tested, including those from cattle in the clinical stages of disease. Notably, further analysis of the protein encoded by MAP0862 showed that it reacted with sera from additional infected cattle but not with sera from uninfected control animals. The fusion product of MAP0860c did not react with any of the sera tested, while the remaining four proteins were variably recognized by sera from M. avium subsp. paratuberculosis-infected cattle. Collectively, the results of this study demonstrate the utility of genomic data to identify potential diagnostic sequences.

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Paratuberculosis in cattle: A comparison of three serologic tests with results of fecal culture

Cited by 55 publications

References 1 publication

Assessment ofDietziasubsp.C79793-74for treatment of cattle with evidence of paratuberculosis

Assessment ofDietziasubsp.C79793-74for treatment of cattle with evidence of paratuberculosis

The complete genome sequence ofMycobacterium aviumsubspeciesparatuberculosis

Characterization of Novel Coding Sequences Specific to Mycobacterium avium subsp. paratuberculosis : Implications for Diagnosis of Johne's Disease

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