Paratransgenesis applied to control insect-transmitted plant pathogens

Miller, Thomas A.; Durvasula, Ravi; Matthews, Scott; Lauzon, Carol R.; Lampe, David J.

doi:10.1201/9781420005936.ch15

Cited by 12 publications

(19 citation statements)

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“…Insect symbionts are currently being explored in the framework of symbiont‐based control . Symbiont‐based control (or symbiotic control) uses symbiotic microorganisms to develop strategies to suppress the population of a pest species or to control a disease (Miller et al . 2006).…”

Section: Wolbachia‐induced Cytoplasmic Incompatibility and Applicationsmentioning

confidence: 99%

“…2004b), mosquito populations (Xi et al . 2005; Ruang‐areerate & Kittayapong 2006) and aflatoxin produced by Aspergillus flavus (Miller et al . 2006).…”

Section: Wolbachia‐induced Cytoplasmic Incompatibility and Applicationsmentioning

confidence: 99%

“…2006). Paratransgenesis is a branch of symbiont‐based control where a symbiotic microorganism is genetically altered rather than its host (Miller et al . 2006).…”

Section: Wolbachia‐induced Cytoplasmic Incompatibility and Applicationsmentioning

confidence: 99%

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Insect symbionts and applications: The paradigm of cytoplasmic incompatibility‐inducing Wolbachia

Ioannidis

Bourtzis

2007

Entomological Research

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Wolbachia is a group of Gram‐negative, obligatory intracellular and maternally transmitted alpha‐Proteobacteria. They have been reported to establish symbiotic relationships with a great variety of species of the most diverse animal class, the insects, as well as with several other arthropods and with filarial nematodes. The reproductive alterations Wolbachia causes in its hosts account for its widespread distribution. These alterations include parthenogenesis, feminization, male killing, and cytoplasmic incompatibility (CI). CI is the most frequent and best studied effect Wolbachia has on its hosts. CI is a form of male sterility, ultimately resulting in embryo lethality in diplodiploid host species. As a consequence of CI, Wolbachia infections spread and lead to the replacement of uninfected populations. CI was used nearly four decades ago to control important disease vectors with very encouraging results, and a number of more recent studies have confirmed the effectiveness of CI as a pest population suppression tool as well as a driving mechanism. Furthermore, recent advancements in the field encourage the development of Wolbachia‐based methods for the biological control of insect pests and disease vectors of agricultural, environmental and medical importance.

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Section: Wolbachia‐induced Cytoplasmic Incompatibility and Applicationsmentioning

confidence: 99%

“…2004b), mosquito populations (Xi et al . 2005; Ruang‐areerate & Kittayapong 2006) and aflatoxin produced by Aspergillus flavus (Miller et al . 2006).…”

Section: Wolbachia‐induced Cytoplasmic Incompatibility and Applicationsmentioning

confidence: 99%

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Insect symbionts and applications: The paradigm of cytoplasmic incompatibility‐inducing Wolbachia

Ioannidis

Bourtzis

2007

Entomological Research

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“…We have since applied this technique to a number of other arthropod systems, including sand fly-transmitted leishmaniasis4 and sharpshooter-mediated Pierce's disease of grapes5. We are further developing a modification of this procedure to control diseases in shrimp mariculture6.…”

Section: Introductionmentioning

confidence: 99%

Paratransgenic Control of Vector Borne Diseases

Hurwitz¹,

Fieck²,

Read³

et al. 2011

Int. J. Biol. Sci.

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Conventional methodologies to control vector borne diseases with chemical pesticides are often associated with environmental toxicity, adverse effects on human health and the emergence of insect resistance. In the paratransgenic strategy, symbiotic or commensal microbes of host insects are transformed to express gene products that interfere with pathogen transmission. These genetically altered microbes are re-introduced back to the insect where expression of the engineered molecules decreases the host's ability to transmit the pathogen. We have successfully utilized this strategy to reduce carriage rates of Trypanosoma cruzi, the causative agent of Chagas disease, in the triatomine bug, Rhodnius prolixus, and are currently developing this methodology to control the transmission of Leishmania donovani by the sand fly Phlebotomus argentipes. Several effector molecules, including antimicrobial peptides and highly specific single chain antibodies, are currently being explored for their anti-parasite activities in these two systems. In preparation for eventual field use, we are actively engaged in risk assessment studies addressing the issue of horizontal gene transfer from the modified bacteria to environmental microbes.

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“…Since our initial experiments, we have adapted the paratransgenic strategy to numerous other vector-borne disease systems, including sand-fly-mediated leishmaniasis [26,27] and sharpshooter-mediated Pierce's disease [28]. The strategy is also being developed and extrapolated into shrimp mariculture [29].…”

Section: The Paratransgenic Approach An Alternative Strategy To Contmentioning

confidence: 99%

A Paratransgenic Strategy for the Control of Chagas Disease

Hurwitz

Fieck

Klein

et al. 2012

Psyche: A Journal of Entomology

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Chagas disease results from infection with the parasiteTrypanosoma cruzi. This disease remains a significant cause of morbidity and mortality in central and south America. Chagas disease now exists and is detected worldwide because of human migration. Control of Chagas disease has relied mainly on vector eradication however, the development of insect resistance to pesticides, coupled with cost and adverse health effects of insecticide treatments, has prompted our group to investigate novel methods of transmission control. Our laboratory has been instrumental in the development of the paratransgenic strategy to control vectorial transmission ofT. cruzi. In this paper, we discuss various components of the paratransgenic approach. Specifically, we describe classes of molecules that can serve as effectors, including antimicrobial peptides, endoglucanases, and highly specific single chain antibodies that target surface glycoprotein tags on the surface ofT. cruzi. Furthermore, we address evolving concepts related to field dispersal of engineered bacteria as part of the paratransgenic control strategy and attendant risk assessment evaluation.

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Paratransgenesis applied to control insect-transmitted plant pathogens

Cited by 12 publications

References 0 publications

Insect symbionts and applications: The paradigm of cytoplasmic incompatibility‐inducing Wolbachia

Insect symbionts and applications: The paradigm of cytoplasmic incompatibility‐inducing Wolbachia

Paratransgenic Control of Vector Borne Diseases

A Paratransgenic Strategy for the Control of Chagas Disease

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