Parathyroid Hormone-Related Protein Is a Factor in Normal Fish Pituitary

Danks, Janine A.; Devlin, A J; Ho, P.; Diefenbach-Jagger, H.; Power, Deborah M.; Canário, Adelino V.M.; Martın, Thomas; Ingleton, P. M.

doi:10.1006/gcen.1993.1156

Cited by 55 publications

(44 citation statements)

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“…The specificity of these antibodies for reaction with PTHrP and not PTH were confirmed by Western blotting, radioimmunoassay, and in biological assays as previously described (Danks et al, 1989(Danks et al, , 1990(Danks et al, , 1993.…”

Section: Immunohistochemistrymentioning

confidence: 91%

“…The methods of immunohistochemistry for light microscopy were based on the technique of Sternberger (1974) and have been described in detail in Danks et al (1989Danks et al ( , 1990Danks et al ( , 1993. Briefly, after dewaxing sections were rehydrated through graded alcohols, and heavy metals from the fixative removed by 1% iodine in ethanol and decolorized in 5% sodium thiosulphate before washing in running tap water.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Sections were dehydrated through alcohols, cleared in xylene, and mounted in piccolyte or Depex. Negative controls included nonimmune rabbit serum substituted for primary immune antibody and primary antibody blocked with PTHrP antigen with a section of normal human skin as positive control, all as described in Danks et al (1993). Antibody to human PTH (Biogenex) was also used as described in Danks et al (1993).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Sacci were incubated for a number of days to see if immunodetectable PTHrP was released into the medium as it is from sea bream pituitaries (Danks et al, 1993). Incubations were carried out at 18°C.…”

Section: Incubation Of Sea Bream Sacci Vasculosimentioning

confidence: 99%

“…Immunodetectable PTHrP is present in a variety of tumors, especially those of squamous cell origin (Danks et al, 1989); however, it is also found in a range of normal cells, particularly human keratinocytes (Danks et al, 1989). PTHrP can be detected in the pituitary and serum of a marine teleost, the sea bream (S. aurata), and it appears to be secreted by the pituitary in vitro (Danks et al, 1993). During the latter studies some cells of the saccus vasculosus epithelium also gave positive reactions with antiserum to PTHrP; these studies are now extended.…”

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Immunochemical Detection of Parathyroid Hormone-Related Protein in the Saccus Vasculosus of a Teleost Fish

Devlin¹,

Danks

Faulkner³

et al. 1996

General and Comparative Endocrinology

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Using antisera to regions of human parathyroid hormone-related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and waxembedded SV tissue reacted with antisera to the prepro region of human PTHrP (−13 to +2), the N-terminus PTHrP (1-16), and the midmolecule PTHrP (50-69). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and incubation media contained two major proteins of 14.3 and 15 kDa. By Western blotting these two proteins both reacted with the three antisera used for IHC, suggesting that they are immunochemically similar to human PTHrP (1-84). Ultrastructurally the coronet cells of Sparus saccus vasculosus resembled coronet cells described for other teleosts, with an abundant smooth endoplasmic reticulum (SER) which was more highly organized in the coronets. IHC at EM level showed reaction mainly with the membranes of the SER. These results suggest that S. aurata saccus vasculosus may produce a PTHrP-like molecule similar to human PTHrP.

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Section: Immunohistochemistrymentioning

confidence: 91%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Incubation Of Sea Bream Sacci Vasculosimentioning

confidence: 99%

mentioning

confidence: 99%

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Immunochemical Detection of Parathyroid Hormone-Related Protein in the Saccus Vasculosus of a Teleost Fish

Devlin¹,

Danks

Faulkner³

et al. 1996

General and Comparative Endocrinology

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Parathyroid hormone-related protein (PTHrP) in cartilaginous and bony fish tissues

et al. 1999

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Tissues from a range of fish were examined for the presence of parathyroid hormone‐related protein (PTHrP) to investigate PTHrP protein distribution and PTHrP gene expression in jawless fish, cartilaginous fish, and bony fish. Immunoreactive PTHrP was localized using antisera to N‐terminal and mid‐molecule regions of human PTHrP and PTHrP gene expression examined using a digoxigenin labeled riboprobe to a conserved region of the mammalian PTHrP gene. In all of the fish studied, PTHrP protein and messenger RNA (mRNA) were localized to the skin, kidney, and skeletal muscle, following the pattern seen in higher vertebrates. Additional sites of localization for both protein and mRNA included gill, nerve cord, and pituitary, as well as developing dermal denticles and rectal gland in the elasmobranch species. The sites of PTHrP distribution indicate that PTHrP may have roles in ionoregulation as well as growth and differentiation in fish, as has been suggested in higher vertebrates. The results imply that the distribution of PTHrP is widespread in fish and that there is homology between the PTHrP molecules found in humans and fish. The conservation of localization and possible similarity of the PTHrP molecules between tetrapods and fish suggests that PTHrP has a number of fundamental roles in vertebrates. J. Exp. Zool. 284:541–548, 1999. © 1999 Wiley‐Liss, Inc.

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Roles of Parathyroid Hormone and Parathyroid Hormone–Related Peptide in Calcium Metabolism and Bone Biology: Biological Actions and Receptors

Jüppner

Potts

2000

Comprehensive Physiology

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The sections in this article are: Parathyroid Hormone Chemistry Peripheral Metabolism Actions in Kidney and Bone Parathyroid Hormone‐Related Peptide Chemistry: Role of the Amino‐terminal Portion in Calcium Metabolism Biological Role in Bone Development Receptors That Mediate Analogous and Distinct Molecular Actions of Parathyroid Hormone and Parathyroid Hormone–Related Peptide The Parathyroid Hormone/Parathyroid Hormone–Related Peptide Receptor The Type 2 Parathyroid Hormone Receptor Additional Receptors Structure‐Based Design of Analogues Summary

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Parathyroid Hormone-Related Protein Is a Factor in Normal Fish Pituitary

Cited by 55 publications

References 0 publications

Immunochemical Detection of Parathyroid Hormone-Related Protein in the Saccus Vasculosus of a Teleost Fish

Immunochemical Detection of Parathyroid Hormone-Related Protein in the Saccus Vasculosus of a Teleost Fish

Parathyroid hormone-related protein (PTHrP) in cartilaginous and bony fish tissues

Roles of Parathyroid Hormone and Parathyroid Hormone–Related Peptide in Calcium Metabolism and Bone Biology: Biological Actions and Receptors

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