Parathyroid Hormone-Related Peptide Stimulates Proliferation of Highly Tumorigenic Human SV40-Immortalized Breast Epithelial Cells

Cataisson, Christophe; Lieberherr, Michèle; Cros, Magali; Gauville, C; Graulet, AM; Cotton, J. P.; Calvo, Fabien; Vernejoul, M. C. De; Foley, John; Bouizar, Zhor

doi:10.1359/jbmr.2000.15.11.2129

Cited by 33 publications

(36 citation statements)

References 45 publications

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“…Other more recent studies using monoclonal antibodies detected positive signals in 100% of invasive carcinomas tested (Iezzoni et al 1998;Surowiak et al, 2003) .Similarly, both PTHrP and its receptor PTH1-R have been detected in breast cancers (Carron et al, 1997, Downey et al, 1997, Iezzoni et al 1998Linforth et al 2002 ). There is now considerable evidence that PTHrP influences the growth and differentiation of mammary tissue, both in vitro and in vivo (Wysolmerski,et al 1995, Ferrari, et al 1992, Birch,et al 1995, Lupareilo et al 1997, Cataisson et al 2000. PTHrP is secreted by epithelial cells in the breast (Sebag et al 1994) and that both normal myoepithelial and tumoral epithelial mammary cells (Seitz et al 1993) secrete PTHrP in vitro (Birch,et al 1995Luparello et al 1997,Cataisson et al 2000.…”

Section: Pthrp and Breast Cancermentioning

confidence: 99%

“…There is now considerable evidence that PTHrP influences the growth and differentiation of mammary tissue, both in vitro and in vivo (Wysolmerski,et al 1995, Ferrari, et al 1992, Birch,et al 1995, Lupareilo et al 1997, Cataisson et al 2000. PTHrP is secreted by epithelial cells in the breast (Sebag et al 1994) and that both normal myoepithelial and tumoral epithelial mammary cells (Seitz et al 1993) secrete PTHrP in vitro (Birch,et al 1995Luparello et al 1997,Cataisson et al 2000. Analyses of human breast cancer cell lines and immunological and in situ hybridization studies of human tumors indicate that neoplasic epithelial cells express both PTHrP and the PTH/PTHrP receptor (Birch,et al 1995,Iezzoni et al 1998, Cataison et al, 2000.…”

Section: Pthrp and Breast Cancermentioning

confidence: 99%

“…Bone metastases of breast cancers are predominantly osteolytic and cause skeletal lesions leading to fractures, intractable bone pain, nerve compression, and hypercalcemia (Arguello et al 1988, Bouizar et al, 1993. In vitro studies identified PTHrP in primary cultures of mammary tumors and in breast cancer cell lines, although the amount varied from one line to another (Birch et al 1995;Guise et al 1996;Cataisson et al, 2000, Saito et al, 2005. Breast cancer cells frequently spread to bone, where they form osteolytic metastases.…”

Section: Parathyroid Hormone In Invasive Breast Cancer 311 Role Of mentioning

confidence: 99%

“…It is not only linked to the development of bone metastases; it concentration is also increased when breast tumor cells metastasize to bone (Bundred et al 1992) Metastasis is a complex process involving a cascade of relatively well known cellular events . Many factors (Guise et al, 1996 and, including PTHrP, can facilitate metastasis to bone (Vargas et al 1992;, Guise et al, 2002, Bouizar et al, 1993, Cataisson et al , 2000 (Figure 4) .These factors promote the differentiation and activation of osteoclasts, and hence bone resorption. There is considerable evidence that PTHrP promotes metastases to bone (Guise et al 1996(Guise et al , 2002.…”

Section: Parathyroid Hormone In Invasive Breast Cancer 311 Role Of mentioning

confidence: 99%

“…In contrast, S2T2 cells, which also did not grow in soft agar, produced slow growing tumors (<8 mm in diameter 8 to 10 weeks after inoculation) in nude mice. The NS2T2A1 cell line was derived from an S2T2 tumor that had been grown in nude mice, reestablished in vitro and then repassaged in nude mice (Berthon et al 1992, Cataisson et al 2000. The NS2T2A1 line produced colonies in soft agar and tumors that grew rapidly in nude mice.…”

Section: Pthrp In Different Breast Cell Modelsmentioning

confidence: 99%

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Parathyroid Hormone Related Protein: A Marker of Breast Tumor Progression and Outcome

Bouizar¹

2011

Breast Cancer - Recent Advances in Biology, Imaging and Therapeutics

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Section: Pthrp and Breast Cancermentioning

confidence: 99%

Section: Pthrp and Breast Cancermentioning

confidence: 99%

Section: Parathyroid Hormone In Invasive Breast Cancer 311 Role Of mentioning

confidence: 99%

Section: Parathyroid Hormone In Invasive Breast Cancer 311 Role Of mentioning

confidence: 99%

Section: Pthrp In Different Breast Cell Modelsmentioning

confidence: 99%

See 3 more Smart Citations

Parathyroid Hormone Related Protein: A Marker of Breast Tumor Progression and Outcome

Bouizar¹

2011

Breast Cancer - Recent Advances in Biology, Imaging and Therapeutics

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Over‐expression of parathyroid hormone Type 1 receptor confers an aggressive phenotype in osteosarcoma

Yang

Hoang

Kubo

et al. 2007

Intl Journal of Cancer

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Osteosarcoma is the most common primary bone malignancy in children and is associated with rapid bone growth. Parathyroid hormone-related peptide (PTHrP) signaling via parathyroid hormone Type 1 receptor (PTHR1) is important for skeletal development and is involved in bone metastases in other tumors. The aim of this study was to investigate the status of PTHrP/PTHR1 and its possible role in osteosarcoma. In a preliminary screening, a higher level of PTHR1 mRNA, but not PTHrP, was found in 4 osteosarcoma xenografts as compared with 4 standard cell lines, or 5 patient derived cell lines (p < 0.05) using quantitative RT-PCR. It was therefore extended to 55 patient specimens, in which a significantly higher level of PTHR1 mRNA was detected in metastatic or relapsed samples than those from primary sites (p < 0.01). Cell behavior caused by PTHR1 overexpression was further studied in vitro using PTHR1 transfected HOS cell line as a model. Over-expression of PHTR1 resulted in increased proliferation, motility and Matrigel invasion without addition of exogenous PTHrP suggesting an autocrine effect. Importantly, the aggressiveness in PTHR1-expressing cells was completely reversed by RNAi mediated gene knockdown. In addition, PTHR1 overexpression led to delayed osteoblastic differentiation and upregulation of genes involved in extracellular matrix production, such as TGF-b1 and connective tissue growth factor. When cocultured with bone marrow derived monocytes, PTHR1 transfected HOS cells induced a greater number of osteoclasts. This study suggests that PTHR1 over-expression may promote osteosarcoma progression by conferring a more aggressive phenotype, and forming a more favorable microenvironment. ' 2007 Wiley-Liss, Inc.Key words: parathyroid hormone Type 1 receptor; metastasis; osteosarcoma Osteosarcoma is typically a high-grade malignancy that has a peak incidence in children and young adults. More than 75% of osteosarcoma in adolescents occurs near growing joints, such as the distal femur, proximal tibia and proximal humerus, implying an association with rapid bone growth.1 Osteosarcoma has a tendency to metastasize early. The 5-year event free survival of osteosarcoma has been improved dramatically from 10% to nearly 70% for patients without detectable metastases at initial diagnosis with the adoption of neo-adjuvant and adjuvant chemotherapy, which is aimed at eliminating microscopic metastatic disease and making primary tumor more amenable for limb salvage procedures. 2,3However, for patients with radiographically detectable metastases or relapse, cure is extremely difficult to achieve even using the regimens of intensified chemotherapy that have being explored during the past 2 decades.4,5 Therefore, identification of factors contributing to tumor progression and metastatic behavior will be essential to improve the outcome for patients with this disease.Parathyroid hormone Type 1 receptor (PTHR1) is the common receptor for parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP). PTH and PTHrP sha...

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Tumor expressed PTHrP facilitates prostate cancer‐induced osteoblastic lesions

Liao

Koh

et al. 2008

Intl Journal of Cancer

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Expression of parathyroid hormone-related protein (PTHrP) correlates with prostate cancer skeletal progression; however, the impact of prostate cancer-derived PTHrP on the microenvironment and osteoblastic lesions in skeletal metastasis has not been completely elucidated. In this study, PTHrP overexpressing prostate cancer clones were stably established by transfection of full length rat PTHrP cDNA. Expression and secretion of PTHrP were verified by western blotting and IRMA assay. PTHrP overexpressing prostate cancer cells had higher growth rates in vitro, and generated larger tumors when inoculated subcutaneously into athymic mice. The impact of tumor-derived PTHrP on bone was investigated using a vossicle co-implant model. Histology revealed increased bone mass adjacent to PTHrP overexpressing tumor foci, with increased osteoblastogenesis, osteoclastogenesis and angiogenesis. In vitro analysis demonstrated pro-osteoclastic and pro-osteoblastic effects of PTHrP. PTHrP enhanced proliferation of bone marrow stromal cells and early osteoblast differentiation. PTHrP exerted a pro-angiogenic effect indirectly, as it increased angiogenesis but only in the presence of bone marrow stromal cells. These data suggest PTHrP plays a role in tumorigenesis in prostate cancer, and that PTHrP is a key mediator for communication and interactions between prostate cancer and the bone microenvironment. Prostate cancer-derived PTHrP is actively involved in osteoblastic skeletal progression.

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Parathyroid Hormone-Related Peptide Stimulates Proliferation of Highly Tumorigenic Human SV40-Immortalized Breast Epithelial Cells

Abstract: ABSTRACT

Cited by 33 publications

References 45 publications

Parathyroid Hormone Related Protein: A Marker of Breast Tumor Progression and Outcome

Parathyroid Hormone Related Protein: A Marker of Breast Tumor Progression and Outcome

Over‐expression of parathyroid hormone Type 1 receptor confers an aggressive phenotype in osteosarcoma

Tumor expressed PTHrP facilitates prostate cancer‐induced osteoblastic lesions

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