Parathyroid allotransplantation without immunosuppression

Hasse, C.; Klöck, Gerd; Schlosser, A.; Zimmermann, U.; Rothmund, M.

doi:10.1016/s0140-6736(05)62473-7

Cited by 144 publications

(86 citation statements)

References 3 publications

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“…The animals had been habituated to consume the meal within 5 min. Blood samples were taken at 10 min and immediately before the start of the tests, and at 1, 3,5,7,10,15,20,25,30,45,60,75,90,105, and 120 min after the start of the tests. Blood lost during sampling was replaced by transfusing blood from normal donor rats after every blood sample.…”

Section: Glucose Tolerance Testingmentioning

confidence: 99%

“…During recent years, important advances have been made with this technology. Human trials have been started and showed temporary but pertinent survival of human microencapsulated tissue after allotransplantation of encapsulated parathyroid cells [3] and islets [4]. Also, microencapsulation has been shown to allow for prolonged survival of xenotransplanted islet grafts in both chemically induced and autoimmune diabetic rodents [5], dogs [4], and monkeys [6].…”

Section: Introductionmentioning

confidence: 99%

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Long-term biocompatibility, chemistry, and function of microencapsulated pancreatic islets

et al. 2003

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Section: Glucose Tolerance Testingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-term biocompatibility, chemistry, and function of microencapsulated pancreatic islets

et al. 2003

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“…During recent years, important advances have been made with this technology. The first allotransplantations in humans with encapsulated parathyroid cells and pancreatic islets have been successfully carried out [2,3]. Also, it has been shown to allow for successful xenotransplantation of islet grafts in both chemically induced and autoimmune diabetic rodents [4], dogs [5], and monkeys [6].…”

mentioning

confidence: 99%

Association between macrophage activation and function of micro-encapsulated rat islets

et al. 2003

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Aims/hypothesis. Survival of microencapsulated islet grafts is limited, even when inflammatory reactions against the capsules are restricted to a small portion of less than 10%. Methods. This study investigates both in vivo in rat recipients and in vitro whether cellular overgrowth on this minority of the capsules contributes to limitations in the functional survival of the 90% of the encapsulated islets which remain free of any cellular overgrowth. Results. In successful rat recipients of an allogenic microencapsulated islet graft we found that the vast majority of cells in the capsular overgrowth were activated ED-1 and ED-2 positive macrophages which were found in numbers of approximately 1500 per capsule. Co-culture of encapsulated islets with 1500 (nr8383) rat-macrophages per capsule showed that the activation of macrophages was caused by islet-derived bioactive factors since TNF-α and IL-1β secretion by macrophages was induced by islet-containing capsules and not by empty capsules. This activation of macrophages was associated with a decrease in function of the encapsulated islets as evidenced by a quantitatively reduced (35%) insulin response in static incubation and a slower response in perifusion. Conclusion/interpretation. Present research aims to design strategies for the temporary inhibition of macrophage activation since macrophages are predominantly present in the first two months after implantation. These strategies will serve as a pertinent basis for future clinical application of microencapsulated islets. [Diabetologia (2003) 46:666-673]

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“…Postoperative hypoparathyroidism may be observed in three different variations such as transient (occurs within 3-6 months), protracted (occurs within 1 year) or permanent (occurs after the 1 st year) (1). Because parathyroid hormone (PTH) has multiple metabolic functions, control of hypoparathyroidism symptoms with calcium (Ca) replacement therapy can be very difficult (2). Parathyroid allotransplantation for permanent hypoparathyroidism is a relatively new option for treatment (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, studies involving iso-, allo-, and xenotransplantation of parathyroid tissue in human and animals indicate promising results that go far beyond the preoperative autotransplantation techniques (17, 18). The use of cultured human parathyroid cells is an essential technique for allo-and xeno-transplantation because it provides better engraftment of parathyroid cells by means of better revascularization and a decrease in the expression of the major histocompatibility complex on parathyroid cells (2,19,20). To overcome organ rejection in parathyroid allotransplantation, a number of methods have been proposed, such as short-term immunosuppression and immune-alteration by depletion of passenger leukocytes or preoperative organ culture but unfortunately, those approaches resulted in prolonged but insufficient graft function (21-23).…”

mentioning

confidence: 99%

Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits

Ayşan

Düzköylü²,

Can

et al. 2017

Turk J Surg

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Objective: Parathyroid allotransplantation is a new method for the treatment of permanent hypoparathyrodism. Adenoma cells are not used for transplantation because of the potential for functional or histopathologic transformation. In this study, we transplanted human adenomatous parathyroid cells to rabbits. Material and Methods:Parathyroid adenoma tissue taken from a male patient was cryopreserved and transplanted into seven New Zealand white rabbits (mean weight, 3700±220 g; mean age, 4.5 months) under immunosuppression. The levels of parathormone, calcium and phosphorus were measured before and after transplantation, and the parathyroid cells were observed histopathologically. Results:Mean parathyroid hormone level was 0.5 pg/dL before transplantation and 6.6 pg/dL after transplantation (p<0.05). Preoperative mean calciumlevel was 14.1 mg/dL, and mean phosporus level was 3.5 mg/dL before transplantation while these values were 14.4 mg/dL and 3.3mg/dL, respectively, after transplantation (p>0.05). Morphologic transformation was not observed in parathyroid cells after transplantation. Conclusion:In short-term observation, adenomatous parathyroid cells can function without malignant transformation. In the future, the preliminary methodology in this study may serve as a safe alternative for allotransplantation into patients with permanent hypoparathyroidism.Keywords: Hypoparathyroidism, xenotransplantation, parathyroid adenoma ABSTRACT Turk J Surg 2017; 33: 91-95 DOI: 10.5152/turkjsurg.2017.3427 Cite this paper as:Ayşan E, Düzköylü Y, Can İ, Büyükpınarbaşılı N. Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits. Turk J Surg 2017; 33(2): 91-95 Original InvestigationProcedure for Parathyroid Tissue Donor Patient: A 49-yearold male patient was admitted to the outpatient clinic with malignant hypercalcemia (blood calcium level >11 mg/dL). Ultrasound evaluation of the neck revealed a 3 cm diameter mass inferior to the left lobe of the thyroid tissue. Parathyroid scintigraphy (Tc 99m MIBI) indicated that the mass was a parathyroid adenoma. A small Kocher neck incision was performed, and the mass was excised and then divided into two parts. One part was used for histopathologic evaluation and the other part was cryopreserved according to thetechnique described below for xenotransplantation. The mass was reported as parathyroid adenoma inthe histopathologic evaluation. Cryopreservation and Cell Preparation:Tissue was pooled and cryopreserved in three main steps: cell isolation, cell counting (total number of cells, rate of viability) and finally storing in a liquid nitrogen tank. Tissue samples were immediately placed in ice-chilled RPMI 1640 media after removal, and the cells were isolated. The entire protocol was carried out in sterile conditions in a sterile hood. All solutions and instruments were sterilized. The tissues were gently placed in a steel filter and rinsed with PBS+5% FCS (medium I). The tissues were then smashed with a syringe pi...

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Parathyroid allotransplantation without immunosuppression

Cited by 144 publications

References 3 publications

Long-term biocompatibility, chemistry, and function of microencapsulated pancreatic islets

Long-term biocompatibility, chemistry, and function of microencapsulated pancreatic islets

Association between macrophage activation and function of micro-encapsulated rat islets

Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits

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