Parasphingorhabdus halotolerans sp. nov. isolated from marine sediment in Jeju Island

Kim, So-Jeong; Kim, Minji; Lee, Ki-Eun; Cha, In-Tae; Park, Soo-Je

doi:10.1007/s00203-021-02366-8

Cited by 2 publications

(1 citation statement)

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“…A phylogenetic analysis of WS/DGAT homolog sequences assigned to Alphaproteobacteria and close relatives from genomes showed two clusters, cluster I containing representatives of the Sphingomonadaceae family, whereas cluster II includes members of the Hyphomicrobiales y Rhodobacterales orders ( Fig 5A ). Among the former, Parasphingorhabdus halotolerans JK6, an isolate obtained from marine sediments [ 60 ], shared high identity values (70.2–81.2%) with three sequences of the metagenomic dataset. A complete set of genes encoding homolog sequences of Kennedy pathway enzymes, and genes involved in β-oxidation located adjacent to the ws/dgat homolog were found in scaffold S1-6153 of the OR07 metagenomic dataset ( Fig 5B ).…”

Section: Resultsmentioning

confidence: 99%

High wax ester and triacylglycerol biosynthesis potential in coastal sediments of Antarctic and Subantarctic environments

et al. 2023

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The wax ester (WE) and triacylglycerol (TAG) biosynthetic potential of marine microorganisms is poorly understood at the microbial community level. The goal of this work was to uncover the prevalence and diversity of bacteria with the potential to synthesize these neutral lipids in coastal sediments of two high latitude environments, and to characterize the gene clusters related to this process. Homolog sequences of the key enzyme, the wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT) were retrieved from 13 metagenomes, including subtidal and intertidal sediments of a Subantarctic environment (Ushuaia Bay, Argentina), and subtidal sediments of an Antarctic environment (Potter Cove, Antarctica). The abundance of WS/DGAT homolog sequences in the sediment metagenomes was 1.23 ± 0.42 times the abundance of 12 single-copy genes encoding ribosomal proteins, higher than in seawater (0.13 ± 0.31 times in 338 metagenomes). Homolog sequences were highly diverse, and were assigned to the Pseudomonadota, Actinomycetota, Bacteroidota and Acidobacteriota phyla. The genomic context of WS/DGAT homologs included sequences related to WE and TAG biosynthesis pathways, as well as to other related pathways such as fatty-acid metabolism, suggesting carbon recycling might drive the flux to neutral lipid synthesis. These results indicate the presence of abundant and taxonomically diverse bacterial populations with the potential to synthesize lipid storage compounds in marine sediments, relating this metabolic process to bacterial survival.

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Section: Resultsmentioning

confidence: 99%