Parasitological and immunological aspects of early Ascaris spp. infection in mice

Gazzinelli-Guimarães, Pedro Henrique; Gazzinelli-Guimarães, Ana Clara; Silva, Flaviane Nunes; Mati, Vitor Luís Tenório; Dhom-Lemos, Lucas de Carvalho; Barbosa, Fernando Sérgio; Passos, Lívia Silva Araújo; Gaze, Soraya; Carneiro, Cláudia Martins; Bartholomeu, Daniella Castanheira; Bueno, Lilian Lacerda; Fujiwara, Ricardo Toshio

doi:10.1016/j.ijpara.2013.02.009

Cited by 52 publications

(87 citation statements)

References 63 publications

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“…Another study recorded increased TNF levels in the lung on days 10 and 12 after the A. suum infection. This could be related with increased number of macrophages (a source of TNF) at the infl ammatory sites (Gazzinelli-Guimarães et al, 2013). In our study the high level of TNF-α after Hg intoxication probably together with IL-1β and IL-6 (Broekhuizen et al, 2005) caused a massive infl ammatory response what resulted in mice weakness and cachexia.…”

Section: Discussionsupporting

confidence: 48%

“…The production of IL-5 cytokine was more infl uenced by A. suum infection than by metal intoxication where the up-regulation of IL-5 in Pb intoxicated mice was probably caused by higher parasite burden. The early production of IL-5 might be associated with the immunopathology induced by the fi rst migrating larvae and the protective eosinophil-mediated immune response (Behm & Ovington 2000;Gazzinelli-Guimarães et al, 2013). Except the mercury the Th2 immunomodulative effect of A. suum on the production of a regulatory IL-10 cytokine was stronger.…”

Section: Discussionmentioning

confidence: 99%

“…Due to a higher infectivity rate of A. suum larvae between 100 th and 200 th days of culture a 120 days old A. suum eggs culture was used (Gazzinelli-Guimarães et al, 2013).…”

Section: Infectionmentioning

confidence: 99%

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Heavy metal intoxication compromises the host cytokine response inAscaris Suummodel infection

Dvorožňáková

Šoltýs

2016

Helminthologia

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SummaryLead (Pb), Cadmium (Cd) and Mercury (Hg) are recognized for their deleterious effect on the environment and immunity where subsequently compromised immune response affects the susceptibility to the potential parasitic infections. This study examined the host cytokine response after heavy metal intoxication (Pb, Cd, and Hg) and subsequent Ascaris suum infection in BALB/c mice. Pb modulated murine immune response towards the Th2 type of response (delineated by IL-5 and IL-10 cytokine production) what was also dominant for the outcome of A. suum infection. Chronic intoxication with Pb caused a more intensive development of the parasite infection. Cd stimulated the Th1 immune response what was associated with increase in IFN-γ production and reduction of larvae present in the liver of intoxicated mice. The larval burden was also low in mice intoxicated with Hg. This was probably not related to the biased Th1/Th2 type of immune response, but rather to the bad host conditions caused by mercury toxicity and high level of pro-cachectic cytokine TNF-α.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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Heavy metal intoxication compromises the host cytokine response inAscaris Suummodel infection

Dvorožňáková

Šoltýs

2016

Helminthologia

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“…Since then neutrophils have been shown to participate in the host response to helminths, but it is not always clear if they are mobilized because of concomitant tissue damage [7], the parasite (or its eggs/products) per se [6,23], or bacteria from the helminth as described for Wolbachia endobacteria causing neutrophil accumulation around the filarial nematode, Onchocerca volvulus [24].…”

Section: Discussionmentioning

confidence: 99%

“…Neutrophils have been implicated in an effective response to the larval stages of the nematodes Lithomosoides sigmodontis, Haemonchus contortus, and Strongyloides steroralis, which can involve myeloperoxidase (MPO) and the release of extracellular traps (i.e., proteases embedded in a chromatin net) [2][3][4][5]. Mice infected with Ascaris have increased numbers of neutrophils in their lungs at the peak of helminth migration (and tissue damage) through the airways [6]. Similar findings were presented for Nippostronglyus brasiliensis-infected mice, and suppression of this neutrophil response resulted in reduced mobilization of macrophages capable of killing the parasite and alternatively activated macrophages (AAMs) [7].…”

Section: Introductionmentioning

confidence: 99%

A Trypsin-Sensitive Proteoglycan from the Tapeworm <b><i>Hymenolepis diminuta</i></b> Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation

et al. 2018

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It has emerged that neutrophils can play important roles in the host response following infection with helminth parasites. Mice infected with the tapeworm, Hymenolepis diminuta, are protected from some inflammatory conditions, accompanied by reduced neutrophil tissue infiltration. Thus, the ability of a phosphate-buffered saline-soluble extract of the worm (H. diminuta extract [HdE]) was tested for (1) its ability to activate murine neutrophils (Ca²⁺ mobilization, reactive oxygen species (ROS) and cytokine production); and (2) affect neutrophil chemotaxis in vitro to the penta-peptide, WKYMVm, the chemokine, KC, and leukotriene B₄. HdE was not cytotoxic to neutrophils, elicited a Ca²⁺ response and ROS, but not, cytokine (KC, interleukin-10, tumour necrosis factor-α) generation. HdE is not a chemotactic stimulus for murine neutrophils. However, a heat- and trypsin-sensitive, acid-insensitive proteoglycan (sensitive to sodium metaperiodate) in the HdE significantly reduced neutrophil chemotaxis towards WKYMVm or KC, but not LTB₄. The latter suggested that the HdE interfered with p38 mitogen-activated protein kinase signalling, which is important in WKYMVm chemotaxis. Corroborating this, immunoblotting revealed reduced phosphorylated p38, and the downstream signal heat-shock protein-27, in protein extracts from HdE + WkYMVm treated cells compared to those exposed to the penta-peptide only. We speculate that HdE can be used to modify the outcome of neutrophilic disease and that purification of the bioactive proteoglycan(s) from the extract could be used as a template to design immunomodulatory drugs targeting neutrophils.

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Overview on Ascariasis in Humans in South Asia

Deslyper

Holland

2017

Neglected Tropical Diseases

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Parasitological and immunological aspects of early Ascaris spp. infection in mice

Cited by 52 publications

References 63 publications

Heavy metal intoxication compromises the host cytokine response inAscaris Suummodel infection

Heavy metal intoxication compromises the host cytokine response inAscaris Suummodel infection

A Trypsin-Sensitive Proteoglycan from the Tapeworm <b><i>Hymenolepis diminuta</i></b> Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation

Overview on Ascariasis in Humans in South Asia

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