PARASITISM OF PHOTOSYNTHETIC DINOFLAGELLATES BY THREE STRAINS OF <i>AMOEBOPHRYA</i> (DINOPHYTA): PARASITE SURVIVAL, INFECTIVITY, GENERATION TIME, AND HOST SPECIFICITY<sup>1</sup>

Coats, D. Wayne; Park, Myung Gil

doi:10.1046/j.1529-8817.2002.01200.x

Cited by 140 publications

(181 citation statements)

References 22 publications

(38 reference statements)

Supporting

Mentioning

177

Contrasting

Order By: Relevance

“…In total, 7,388 genes, including a wealth of genes related to cell division, reconstruction, and proliferation, are expressed at the 96-h stage (see Data set S1 in the supplemental material). During this life stage, the parasitoid trophont undergoes karyokinesis, and each cell forms its own flagellum (26).…”

Section: Discussionmentioning

confidence: 99%

“…S1 in the supplemental material). Infection of the host culture was established following the methods of Coats and Park (26). Infective parasitoid dinospores for the experiment were harvested from an infected host culture by gravity filtration through a 10-m-pore-size mesh.…”

Section: Culturesmentioning

confidence: 99%

“…Samples (10 ml) were fixed with formaldehyde (10% CaCO 3 -buffered formaldehyde; 2% final concentration), and three 1-ml aliquots were counted in duplicate after sedimentation in chambers using a microscope (Zeiss Axiovert 200M) (26). Parasitoid prevalence (i.e., the percentage of infected host cells) was assessed by detecting well-maturing parasitoids using the natural autofluorescence of the parasitoid (29).…”

Section: Culturesmentioning

confidence: 99%

“…A motile vermiform stage of brief duration, composed of several rows of biflagellate cells with concerted swimming behavior, is then released from the host after the intracellular maturation of the parasitoid. Soon after this release, this structure dissociates into hundreds of free-living infective dinospores (26,29).…”

mentioning

confidence: 99%

“…The life cycle begins with small infective biflagellate cells termed dinospores (22,25,26) (see Fig. S1 in the supplemental material).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Wohlrab

Glöckner

et al. 2014

Eukaryot Cell

View full text Add to dashboard Cite

The regulatory circuits during infection of dinoflagellates by their parasites are largely unknown on the molecular level. Here we provide molecular insights into these infection dynamics. Alexandrium tamarense is one of the most prominent harmful algal bloom dinoflagellates. Its pathogen, the dinoflagellate parasitoid Amoebophrya sp., has been observed to infect and control the blooms of this species. We generated a data set of transcripts from three time points (0, 6, and 96 h) during the infection of this parasite-host system. Assembly of all transcript data from the parasitoid (>900,000 reads/313 Mbp with 454/Roche next-generation sequencing [NGS]) yielded 14,455 contigs, to which we mapped the raw transcript reads of each time point of the infection cycle. We show that particular surface lectins are expressed at the beginning of the infection cycle which likely mediate the attachment to the host cell. In a later phase, signal transduction-related genes together with transmembrane transport and cytoskeleton proteins point to a high integration of processes involved in host recognition, adhesion, and invasion. At the final maturation stage, cell division-and proliferation-related genes were highly expressed, reflecting the fast cell growth and nuclear division of the parasitoid. Our molecular insights into dinoflagellate parasitoid interactions point to general mechanisms also known from other eukaryotic parasites, especially from the Alveolata. These similarities indicate the presence of fundamental processes of parasitoid infection that have remained stable throughout evolution within different phyla.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Culturesmentioning

confidence: 99%

Section: Culturesmentioning

confidence: 99%

mentioning

confidence: 99%

“…The life cycle begins with small infective biflagellate cells termed dinospores (22,25,26) (see Fig. S1 in the supplemental material).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Wohlrab

Glöckner

et al. 2014

Eukaryot Cell

View full text Add to dashboard Cite

show abstract

Introduction and Overview

Kirchman

2008

Microbial Ecology of the Oceans

View full text Add to dashboard Cite

Phylogenetic composition and distribution of picoeukaryotes in the hypoxic northwestern coast of the Gulf of Mexico

2012

View full text Add to dashboard Cite

Coastal marine hypoxic, or low-oxygen, episodes are an increasing worldwide phenomenon, but its effect on the microbial community is virtually unknown by far. In this study, the community structure and phylogeny of picoeukaryotes in the Gulf of Mexico, which are exposed to severe hypoxia in these areas was explored through a clone library approach. Both oxic surface waters and suboxic bottom waters were collected in August 2010 from three representative stations on the inner Louisiana shelf near the Atchafalaya and Mississippi River plumes. The bottom waters of the two more western stations were much more hypoxic in comparison to those of the station closest to the Mississippi River plume, which were only moderately hypoxic. A phylogenetic analysis of a total 175 sequences, generated from six 18S rDNA clone libraries, demonstrated a clear dominance of parasitic dinoflagellates from Marine alveolate clades I and II in all hypoxic waters as well as in the surface layer at the more western station closest to the Atchafalaya River plume. Species diversity was significantly higher at the most hypoxic sites, and many novel species were present among the dinoflagellate and stramenopile clades. We concluded that hypoxia in the Gulf of Mexico causes a significant shift in picoeukaryote communities, and that hypoxia may cause a shift in microbial food webs from grazing to parasitism.

show abstract

PARASITISM OF PHOTOSYNTHETIC DINOFLAGELLATES BY THREE STRAINS OF AMOEBOPHRYA (DINOPHYTA): PARASITE SURVIVAL, INFECTIVITY, GENERATION TIME, AND HOST SPECIFICITY¹

Cited by 140 publications

References 22 publications

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Introduction and Overview

Phylogenetic composition and distribution of picoeukaryotes in the hypoxic northwestern coast of the Gulf of Mexico

Contact Info

Product

Resources

About

PARASITISM OF PHOTOSYNTHETIC DINOFLAGELLATES BY THREE STRAINS OF AMOEBOPHRYA (DINOPHYTA): PARASITE SURVIVAL, INFECTIVITY, GENERATION TIME, AND HOST SPECIFICITY1

Cited by 140 publications

References 22 publications

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp

Introduction and Overview

Phylogenetic composition and distribution of picoeukaryotes in the hypoxic northwestern coast of the Gulf of Mexico

Contact Info

Product

Resources

About

PARASITISM OF PHOTOSYNTHETIC DINOFLAGELLATES BY THREE STRAINS OF AMOEBOPHRYA (DINOPHYTA): PARASITE SURVIVAL, INFECTIVITY, GENERATION TIME, AND HOST SPECIFICITY¹