Parasitic Helminth Sample Preparation for Taxonomic Study

Thaenkham, Urusa; Chaisiri, Kittipong; Hui En Chan, Abigail

doi:10.1007/978-981-19-1786-8_9

Cited by 1 publication

(1 citation statement)

References 34 publications

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“…For the preparation of permanent mounting sheets, the helminths were placed for one hour in acetocarmine, and then removed from the dye and washed in 70% ethyl alcohol to eliminate excess dye, followed by dehydration in a graded ethanol series, cleared in clove oil, and finally mounted in Canada balsam. Copepod crustaceans were rinsed in pure glycerin for a period of two weeks (Hayward & Rohde, 1999;Eiras et al, 2006;Almeida & Almeida, 2014;Thaenkham et al, 2022;Wood et al, 2023). The identification of monogeneans, trematodes, and copepods parasites was based on specialized publications (Gibson et al, 2002;Kohn et al, 2006Kohn et al, , 2007Gibbons, 2010;Cohen et al, 2013;Luque et al, 2016;Eiras et al, 2017;Mendoza-Garfi et al, 2017;Smit et al, 2019).…”

Section: Metazoan Parasitesmentioning

confidence: 99%

Ectoparasitic community of the gills of Pacific sierra Scomberomorus sierra Jordan & Starks, 1895 (Actinopteri: Scombridae) from northern Peru

Ferré-Alcántara,

Minaya,

Alvariño

et al. 2023

MACN

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Scombrids have mainly been studied for systematic, zoogeographic and parasitological purposes; however, there are few studies focused on the interaction between ecological parameters and the parasite community of a given host. Therefore, the objective of this research was to analyze the ectoparasite community of Scomberomorus from Northern Peru. A total of 58 specimens of S. sierra were obtained and were necropsied to search for parasites, which were collected and preserved. The main parasitological indices, degree of aggregation, parasite association with size, weight and sex, and finally, the alpha diversity indices of these parasitic fauna were calculated. 98% of the fish were parasitized by at least one parasitic species. The parasites with the highest prevalence were Didymocylindrus sp. (Trematoda) and Thoracocotyle crocea (Monogenea), while the parasite with the highest average intensity was Caligus pelamydis (Copepoda). The mean intensity and abundance of infection of T. crocea and unidentified Didymozoidae. were found to be positively related to the size of S. sierra. Sex showed significant differences only with the mean abundance of T. crocea, and with the mean intensity of Didymocylindrus sp. The five new records of parasites in S. sierra from Peru were Didymocylindrus sp., Glomeritrema sp., unidentified Didymozoidae, Scomberocotyle scomberomori, and C. pelamydis. This study represents the first ecologicalparasitic record for S. sierra in northern Peru.

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