Parasites of sentinel bivalves in the NOAA status and trends program: Distribution and relationship to contaminant body burden

Kim, Yungkul; Powell, Eric N.; Wade, Terry L.; Presley, B.J.; Sericano, José L.

doi:10.1016/s0025-326x(98)00131-3

Cited by 41 publications

(28 citation statements)

References 21 publications

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“…Moreover, since MLR/MET values were >1.2 µm µm -1 throughout the studied period, it seems that mussels were subjected to some source of environmental stress (Cajaraville et al 1992. Histopathological alterations are useful to assess the general health status of sentinel molluscs and hence ecosystem health (Kim et al 1998). Mussels commonly harbour parasites and diseases of a variety of types that can significantly affect their health and fecundity (Pérez Camacho et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Garmendia¹,

Soto²,

Cajaraville³

et al. 2010

Aquat. Biol.

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Mussels Mytilus galloprovincialis were collected monthly in 2 localities in the Northern Iberian Peninsula, Oia (Galicia) and Mundaka (Basque Coast), from April 2005 to May 2006, to investigate whether seasonal variability in biomarkers follows the same pattern at both geographical areas. The following battery of biomarkers was analysed: lysosomal membrane stability and structural changes, intracellular neutral lipid accumulation, cell type composition in the digestive gland epithelium, structural changes of digestive alveoli and digestive gland histopathology. Additionally, gonad index was measured as a supporting parameter. Overall, results demonstrate that most studied biomarkers showed similar seasonal variability pattern in both localities. Lysosomal membrane stability (labilisation period [LP]) and most histopathological conditions showed similar seasonal variability patterns in both localities, although values of LP and prevalences of some parasites were higher in Mundaka. Data provide baseline values to be used as reference values for the biomonitoring programmes carried out in coastal areas from Galicia to the Basque Coast. It is concluded that for monitoring programmes based on biomarkers, samples must be taken at least twice per year (spring and autumn) to avoid the effect on biomarkers of the late stages of each reproductive cycle.

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Section: Discussionmentioning

confidence: 99%

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Garmendia¹,

Soto²,

Cajaraville³

et al. 2010

Aquat. Biol.

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“…The Ulrich et al (2007) study did not include histological confirmation of the PCR results, whereas the negative results that we found in our PCR assay were consistent with those from histological examination of the same oysters, as well as previous histological surveys of Crassostrea virginica in the Gulf of Mexico, including the annual Mussel Watch analyses that began in 1995. Since at least 1978, several thousand oysters have been examined histologically without finding any recognizable haplosporidian (Couch 1985, Gauthier et al 1990, Fisher et al 1996, Kim et al 1998, Fisher et al 2000, Winstead et al 2004, Kim & Powell 2006. In addition, at least one other study has examined Gulf of Mexico oysters using both PCR and histology.…”

Section: Discussionmentioning

confidence: 99%

“…Dis Aquat Org 93: [251][252][253][254][255][256] 2011 C. virginica from Nova Scotia, Canada, to Biscayne Bay, Florida, USA (Haskin & Andrews 1988, Barber et al 1997, Stephenson et al 2003, but none of the several histological studies of oysters in the Gulf of Mexico have reported H. nelsoni or any other haplosporidian (Couch 1985, Gauthier et al 1990, Fisher et al 1996, Kim et al 1998, Winstead et al 2004, Kim & Powell 2006. The most comprehensive of these is the annual status and trends (S&T) …”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Widespread survey finds no evidence of Haplosporidium nelsoni (MSX) in Gulf of Mexico oysters

Ford

Paterno²,

Scarpa³

et al. 2011

Dis. Aquat. Org.

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The advent of molecular detection assays has provided a set of very sensitive tools for the detection of pathogens in marine organisms, but it has also raised problems of how to interpret positive signals that are not accompanied by visual confirmation. PCR-positive results have recently been reported for Haplosporidium nelsoni (MSX), a pathogen of the oyster Crassostrea virginica in 31 of 40 oysters from 6 sites in the Gulf of Mexico and the Caribbean Sea. Histological confirmation of the PCR results was not undertaken, and no haplosporidian has been reported from the numerous histological studies and surveys of oysters in the region. To further investigate the possibility that H. nelsoni is present in this region, we sampled 210 oysters from 40 sites around the Gulf of Mexico and Puerto Rico using PCR and 180 of these using tissue-section histology also. None of the oysters showed evidence of H. nelsoni by PCR or of any haplosporidian by histology. We cannot, therefore, confirm that H. nelsoni is present and widespread in the Gulf of Mexico and the Caribbean Sea. Our results do not prove that H. nelsoni is absent from the region, but taken together with results from previous histological surveys, they suggest that for the purposes of controlling oyster importation, the region should continue to be considered free of the parasite. KEY WORDS: PCR · Oyster · Crassostrea virginica · Molecular detection · Infection · Shellfish importation · Haplosporidian Resale or republication not permitted without written consent of the publisherDis Aquat Org 93: [251][252][253][254][255][256] 2011 C. virginica from Nova Scotia, Canada, to Biscayne Bay, Florida, USA (Haskin & Andrews 1988, Barber et al. 1997, Stephenson et al. 2003, but none of the several histological studies of oysters in the Gulf of Mexico have reported H. nelsoni or any other haplosporidian (Couch 1985, Gauthier et al. 1990, Fisher et al. 1996, Kim et al. 1998, Winstead et al. 2004, Kim & Powell 2006. The most comprehensive of these is the annual status and trends (S&T) MATERIALS AND METHODSCrassostrea virginica were collected between December 2007 and February 2008 at 32 sites representing 14 bay systems from lower Laguna Madre, Texas, to Florida Bay, Florida, USA, and C. rhizophorae were collected from 2 bays in Puerto Rico (Fig. 1, Table 1). In May and July 2010, C. virginica were sampled from 6 additional sites, representing 2 bay systems in Texas and 1 in Florida (Fig. 1, Table 1). All samples were express shipped directly to HSRL. Upon receipt, 12 oysters from each sample were opened using a shucking knife, which was rinsed in 10% bleach between each oyster. A section of gill about 4 mm wide and encompassing all 4 demibranchs approximately 6 to 7 mm posterior to the gill-palp junction was excised and preserved in 70% ethanol (EtOH) for PCR analysis. Dissecting instruments were scraped clean in a bleach-sand mixture, dipped in EtOH and flamed between each oyster. Between the PCR section and the palps, and adjacent to the piece of gill ta...

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“…From a previous Mussel Watch Program by the EPA, Yevich and Barszcz (1983) reported correlations between locations with high parasitism and locations high in PCBs. Kim et al (1998) also reported that trematode sporocysts in M. edulis were positively correlated with body burdens of total PCBs. Evidence to support these observations from this study includes the fact that total PCB levels in mytilids were positively associated with infections by trematode sporocysts.…”

Section: Accepted Manuscriptmentioning

confidence: 90%

“…Negative correlations occurred with mirex for the mytilids and with a variety of pesticides and the PCBs in oysters. A negative correlation between cestodes from East-coast oysters with total hexachlorocyclohexane (HCH), a synthetic pesticide that does not occur naturally in the environment, was identi ed by Kim et al (1998). In this study, all potential relationships of cestodes in oysters with manufactured organic chemicals (i.e.…”

Section: Accepted Manuscriptmentioning

confidence: 94%

Relationship of parasites and pathologies to contaminant body burden in sentinel bivalves: NOAA Status and Trends ‘Mussel Watch’ Program

Kim

Powell

Wade

et al. 2008

Marine Environmental Research

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Parasites of sentinel bivalves in the NOAA status and trends program: Distribution and relationship to contaminant body burden

Cited by 41 publications

References 21 publications

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Seasonality in cell and tissue-level biomarkers in Mytilus galloprovincialis: relevance for long-term pollution monitoring

Widespread survey finds no evidence of Haplosporidium nelsoni (MSX) in Gulf of Mexico oysters

Relationship of parasites and pathologies to contaminant body burden in sentinel bivalves: NOAA Status and Trends ‘Mussel Watch’ Program

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