Paraoxonase (Pon1) Q192R polymorphism and serum Pon1 activity in diabetic patients on maintenance hemodialysis

Zhang, B; Eto, Sumiya; Fan, Pengcheng; Bian, Che; Shimoji, Eiso; Saito, T.; Saku, Keijirou

doi:10.5414/cnp60257

Cited by 16 publications

(11 citation statements)

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“…Serum basal paraoxonase (PON1) activity was measured on an autoanalyzer (Hitachi 7600-020S; Hitachi High-Technologies, Tokyo, Japan) with paraoxon as a substrate, as described previously ( 25 ). Plasma PAF-AH activity was measured using an Azwell Auto PAF-AH reagent kit (Alfresa, Osaka, Japan) as described previously ( 26 ).…”

Section: Effects Of Rosuvastatin On Charge-based Ldl and Sdldl Subframentioning

confidence: 99%

Effects of rosuvastatin on electronegative LDL as characterized by capillary isotachophoresis: the ROSARY Study

Zhang

Matsunaga

Rainwater

et al. 2009

Journal of Lipid Research

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Section: Effects Of Rosuvastatin On Charge-based Ldl and Sdldl Subframentioning

confidence: 99%

Effects of rosuvastatin on electronegative LDL as characterized by capillary isotachophoresis: the ROSARY Study

Zhang

Matsunaga

Rainwater

et al. 2009

Journal of Lipid Research

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“…Zhang et al studied the Q192R frequency in patients with type 2 DM with diabetic nephropathy on dialysis, and in non-DM patients on dialysis. 29 Diabetic nephropathy patients on HD displayed significantly lower HDL-C levels and serum PON1 activity than the nondiabetic patients on HD, but there was no difference in the distribution of the Q192R genotypes. Similar to our findings, Reiterová et al 30 excluded the effect of the ET1 G5727 (K198N) polymorphism on the progression of autosomal dominant polycystic kidney disease.…”

Section: Discussionmentioning

confidence: 84%

“…Zhang et al . studied the Q192R frequency in patients with type 2 DM with diabetic nephropathy on dialysis, and in non‐DM patients on dialysis . Diabetic nephropathy patients on HD displayed significantly lower HDL‐C levels and serum PON1 activity than the nondiabetic patients on HD, but there was no difference in the distribution of the Q192R genotypes.…”

Section: Discussionmentioning

confidence: 99%

Genetic polymorphisms and the risk of progressive renal failure in elderly Hungarian patients

Zsom

Fülöp

Zsom

et al. 2011

Hemodialysis International

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The relationship between renal disease progression and genetic polymorphism of enzymes influencing endothelial function remains incompletely understood. We genotyped three cohorts of elderly Hungarian patients: 245 patients with end-stage renal disease (ESRD) on chronic hemodialysis (HD), 88 patients with mild chronic kidney disease (CKD), and 200 healthy controls. The underlying diagnoses of renal diseases were primary glomerulonephritis, interstitial nephritis, hypertension, diabetic nephropathy, and hereditary diseases. We examined genetic polymorphisms of eight candidate genes associated with endothelial function: endothelial constitutive nitric oxide synthase (ecNOS) T-786C, endothelin-1 G5727T, methylenetetrahydrofolate reductase (MTHFR) C677T, paraoxonase-1 Q192R and M55L, angiotensinogen M235T, angiotensin-converting enzyme (ACE) I/D and angiotensin II type 1 receptor A1166C gene. Six gene polymorphisms were detected by real-time polymerase chain reaction with melting-point analysis, and two via allele-specific amplification and gel electrophoresis. Control group patients were in Hardy-Weinberg equilibrium for all tested genotypes. In ESRD patients attributed to hypertension, the endothelin gene G5727T GG genotype occurred significantly less but GT genotype more frequently (P < 0.01 for both). In ESRD patients attributed to primary glomerulonephritis, more ACE DD and less ID genotypes were found (P < 0.02 for both) than in the controls. The underlying diagnosis may modify the association of genetic polymorphism and dialysis-dependent ESRD.

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“…The overexpression of apoA-1 in mice has been associated with a significant reduction in the atherosclerotic process [23,41,43,44]. In addition, Singaraja et al showed that the overexpression of ABCA1 significantly reduces the formation of atherosclerotic lesions in apoE-deficient mice [42].…”

Section: Discussionmentioning

confidence: 99%

“…Feces were collected during the 48-h period and the cholesterol and bile acids were extracted. The mice were euthanized after 48 h and their livers were used for radioactivity measurement (total liver [ 3 H]-cholesterol) [23]. The results are expressed as the percent of the radioactivity injected recovered in plasma, liver, and feces (in cholesterol and bile acids fractions) and the plasma volume was estimated as 3.5% of the animal weight.…”

Section: Methodsmentioning

confidence: 99%

Human paraoxonase 1 overexpression in mice stimulates HDL cholesterol efflux and reverse cholesterol transport

Ikhlef¹,

Berrougui

Simo³

et al. 2017

PLoS ONE

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This study was aimed to investigate the effect of human PON1 overexpression in mice on cholesterol efflux and reverse cholesterol transport. PON1 overexpression in PON1-Tg mice induced a significant 3-fold (p<0.0001) increase in plasma paraoxonase activity and a significant ~30% (p<0.0001) increase in the capacity of HDL to mediate cholesterol efflux from J774 macrophages compared to wild-type mice. It also caused a significant 4-fold increase (p<0.0001) in the capacity of macrophages to transfer cholesterol to apoA-1, a significant 2-fold (p<0.0003) increase in ABCA1 mRNA and protein expression, and a significant increase in the expression of PPARγ (p<0.0003 and p<0.04, respectively) and LXRα (p<0.0001 and p<0.01, respectively) mRNA and protein compared to macrophages from wild-type mice. Moreover, transfection of J774 macrophages with human PON1 also increased ABCA1, PPARγ and LXRα protein expression and stimulates macrophages cholesterol efflux to apo A1. In vivo measurements showed that the overexpression of PON1 significantly increases the fecal elimination of macrophage-derived cholesterol in PON1-Tg mice. Overall, our results suggested that the overexpression of PON1 in mice may contribute to the regulation of the cholesterol homeostasis by improving the capacity of HDL to mediate cholesterol efflux and by stimulating reverse cholesterol transport.

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Paraoxonase (Pon1) Q192R polymorphism and serum Pon1 activity in diabetic patients on maintenance hemodialysis

Cited by 16 publications

References 0 publications

Effects of rosuvastatin on electronegative LDL as characterized by capillary isotachophoresis: the ROSARY Study

Effects of rosuvastatin on electronegative LDL as characterized by capillary isotachophoresis: the ROSARY Study

Genetic polymorphisms and the risk of progressive renal failure in elderly Hungarian patients

Human paraoxonase 1 overexpression in mice stimulates HDL cholesterol efflux and reverse cholesterol transport

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