Paraoxonase lactonase activity (PON-HTLase), asymmetric dimethylarginine (ADMA) and platelet activating factor-acetylhydrolase (PAF-AH) activity in non-obese women with PCOS

Bayrak, Tülin; Dursun, Polat; Bayrak, Ahmet; Gültekın, Murat; Kolusarı, Ali; Çakır, Erdinç; Özyurt, M.sabri; Zeyneloğlu, Hulusi Bülent

doi:10.3109/09513590.2012.683068

Cited by 19 publications

(16 citation statements)

References 26 publications

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“…ADMA belongs to group of methylarginines derived from the degradation of methylated proteins. In addition to higher symmetric dimethylarginine (SDMA) [47], ADMA levels have been shown to be significantly higher in PCOS women [48-51]. DDAH2 enzyme plays a pivotal role in clearance of ADMA in endothelial cells [4].…”

Section: Discussionmentioning

confidence: 99%

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Krishna

Joseph

Thomas

et al. 2017

Cell Physiol Biochem

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Background: Though oxidative stress is associated with Polycystic Ovary Syndrome (PCOS), the status of nitric oxide is still unclear. Nitric Oxide (NO) plays pivotal roles in many physiological functions which are compromised in PCOS. Our recent study reveals lowered T-regulatory cells (Tregs) in PCOS, and Treg generation is known to be regulated by NO levels. However concrete evidences are lacking on mechanisms modulating NO levels under PCOS. Methods: This is a retrospective case-control cohort study, comprised of PCOS women (N=29) and normal menstruating women as controls (N=20). We analysed NOx (nitrite+nitrate) and hydrogen peroxide (H₂O₂) concentrations, transcript levels of endothelial nitric oxide synthase (eNOS)/inducible nitric oxide synthase (iNOS) and arginine modulators, hydrogen peroxide regulators in the cohort. Results: PCOS women showed reduced plasma NOx(nitrate+nitrite) and H₂O₂ compared to controls. We report reduction in transcript levels of iNOS/NOS2 and eNOS/NOS3 in PCOS peripheral blood. The transcripts involved in arginine bioavailability: Argininosuccinate lyase (ASL), Solute Carrier Family1, member 7 (SLC7A1) and Arginase 1 (ARG1) and Asymmetric Dimethyl Arginine (ADMA) metabolism: Protein arginine methyltransferase 1 (PRMT1) and Dimethylarginine dimethylaminohydrolase 2 (DDAH2) also showed differential expression. H₂O₂ concentration in PCOS women was also found to be reduced. The reduction can be attributed to increase in catalase levels as a consequence of the body’s effort to alleviate the oxidative burden in the system. Conclusion: Our study advocates that PCOS women have lowered NO due to reduced iNOS/eNOS expression, low H₂O₂, high ADMA synthesis and reduced arginine bioavailability. An in-depth analysis of redox biology of PCOS to open up potential therapeutic strategies is highly recommended.

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Section: Discussionmentioning

confidence: 99%

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Krishna

Joseph

Thomas

et al. 2017

Cell Physiol Biochem

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“…Reactive oxygen species play an important role in luteolysis in rodent ovaries (22). The decreased levels of Paraoxonase lactonase (antioxidant enzyme), platelet-activating factor acetylhydrolase (antioxidant enzyme preventing LDL oxidation), but elevated asymmetric dimethylation (ADMA as an inhibitor of nitric oxid synthesis) might be a marker of development of atherosclerosis in the PCOS patients associated with insulin resistance (23). Paraoxonase 1 (PON1) as an enzymatic antioxidant prevents lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Ultra-structural Study by Transmission Electron Microscopy: Effect of Omega-3 on Ovary Cell Organelles after Experimental Induced Poly Cystic Ovary Syndrome

Ouladsahebmadarek¹,

Khaki²

2014

IJWHR

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Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done. Materials and Methods: Wistar female rats (n=40) were allocated into four groups, one control (n=10) and three test groups (n=30), that one group received omega-3 (60 mg/rat/orally/daily), second and third groups were induced PCO by single injection of estradiol-valerate (16mg/ kg/ i.m), third group also received omega-3 (240 mg/kg) for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis. Results: vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly (p<0.05) in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly (p<0.05). Conclusion: Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue.

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“…The POase and/or AREase activities are reportedly lower (25,26,27,28) or similar (29,30) and the HTase activity is decreased (31) in PCOS patients compared with control women. However, the level, lactonase activity, and NLA of PON1 have not yet been assessed in PCOS patients.…”

Section: Introductionmentioning

confidence: 91%

Lactonase activity and status of paraoxonase 1 in Chinese women with polycystic ovarian syndrome

Zhang

Liu

et al. 2015

European Journal of Endocrinology

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Objective: To study the relationship between the lactonase activities and status of paraoxonase 1 (PON1) and its association with the PON1 genetic polymorphisms in women with polycystic ovarian syndrome (PCOS). Design: A case-control study. Methods: A total of 455 PCOS patients and 441 control women were included in this study. The lactonase activities and concentrations of PON1 were assayed using 5-thiobutyl butyrolactone (TBBL) and 7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin (DEPCyMC) respectively. A normalized lactonase activity (NLA) was estimated based on the ratio of TBBLase:DEPCyMCase activity. The PON1 genotypes, serum malondialdehyde (MDA) levels and total antioxidant capacity were analyzed. Results: The lactonase activities and levels of PON1 were higher in PCOS patients than in the control women. However, the NLA did not significantly differ between groups. The K108C/T variation of the PON1 gene showed decreased lactonase activities and levels of PON1 in a genotype-dependent manner (CCOCTOTT); the 192Q/R variation of the PON1 gene showed increased PON1 lactonase activities and NLA; and the 55L/M variation of the PON1 gene showed decreased lactonase activities and levels of PON1 but an increased NLA. A multivariable regression analysis showed that the K108C/T, 192Q/R, and 55L/M variations of the PON1 gene, serum apolipoprotein A1, and MDA levels were significant predictors of PON1 lactonase activity, PON1 level, and NLA. Conclusions: The serum lactonase activities and concentrations of PON1 are increased in PCOS patients. The increased oxidative stress and the K108C/T, 192Q/R, and 55L/M genetic polymorphisms of PON1 may be associated with these changes.

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Paraoxonase lactonase activity (PON-HTLase), asymmetric dimethylarginine (ADMA) and platelet activating factor-acetylhydrolase (PAF-AH) activity in non-obese women with PCOS

Abstract: Decreased PON1-HTLase and increased ADMA levels might be a relevant marker for the development of future atherosclerotic heart disease (AHD) in non-obese PCOS patients. Further studies are needed to confirm our results.

Cited by 19 publications

References 26 publications

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Ultra-structural Study by Transmission Electron Microscopy: Effect of Omega-3 on Ovary Cell Organelles after Experimental Induced Poly Cystic Ovary Syndrome

Lactonase activity and status of paraoxonase 1 in Chinese women with polycystic ovarian syndrome

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