Paraoxonase 2 (PON2) expression is upregulated via a reduced-nicotinamide-adenine-dinucleotide-phosphate (NADPH)-oxidase-dependent mechanism during monocytes differentiation into macrophages

Shiner, Maayan; Fuhrman, Bianca; Aviram, Michael

doi:10.1016/j.freeradbiomed.2004.09.003

Cited by 61 publications

(45 citation statements)

References 48 publications

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“…By using Pgk2 as a marker mRNA to detect populations of mRNAs showing similar meiotic expression and translational delays, Ͼ70% of Pgk2 mRNA fractionates as RNPs in the testes of 17-day-old and 22-day-old mice and redistributes onto polysomes in adult mice (Table 10). Fourteen additional mRNAs show similar RNP and polysome distributions (Table 1) (26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36), all of which meet the strict criterion of at least 70% in RNPs in prepuberal testes and show a shift of 20% or more to polysomes in adults. Four genes can be added to this group when we lower the percentage of mRNA in the RNPs of 22-day-old mice from 70% to 62% (Table 1).…”

Section: Many Mrnas That Are Transcribed During Meiosis Move Betweenmentioning

confidence: 94%

Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated

Iguchi

Tobias

Hecht

2006

Proc. Natl. Acad. Sci. U.S.A.

137

145

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T he testis contains a diverse population of somatic and germ cell types. As spermatogenesis proceeds, diploid spermatogonia differentiate into meiotic spermatocytes, which divide twice without additional DNA replication, producing haploid round spermatids (1, 2). These spermatids transform into highly polarized and uniquely shaped spermatozoa. As the germ cells differentiate, the changing amounts and populations of mRNAs in the germ cells and somatic cells have been well documented by microarray analyses (3-7) and by the cloning and sequencing of cDNA libraries prepared from highly purified populations of individual cell types (8,9).Although these microarray and cloning studies provide valuable insight into the temporal appearance͞disappearance of individual mRNAs, they do not address the question of when the proteins encoded by the mRNAs are synthesized. In the germ cells of the testis, a temporal disconnect between mRNA transcription and protein synthesis is especially common, in part because RNA synthesis terminates during midspermiogenesis long before the spermatid completes its differentiation into the spermatozoon (1). Thus, posttranscriptional mechanisms play major roles in the temporal regulation of protein synthesis in developing male gametes.

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Section: Many Mrnas That Are Transcribed During Meiosis Move Betweenmentioning

confidence: 94%

Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated

Iguchi

Tobias

Hecht

2006

Proc. Natl. Acad. Sci. U.S.A.

137

145

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“…Endogenous substrates of PON2 are largely unknown, but it has been shown that its lactonase activity dominantly hydrolyzes the Pseudomonas aeruginosa signaling lactone 3OC12, for which reason PON2 may add to the anti-bacterial defense (16,17), a mechanism apparently sensitive to major calcium disturbances (18,19). Further, studies with PON2-deficient mice indicated that PON2 suppresses atherosclerosis, and several studies described a significant ROS-diminishing function of PON2 in various cells (8,9,18,20,21). Although fundamental to understanding PON2 function, it is yet unanswered whether the lactonase activity and anti-oxidative function are interrelated.…”

Section: Reactive Oxygen Species (Ros)mentioning

confidence: 99%

One Enzyme, Two Functions

Altenhöfer

Witte

Teiber

et al. 2010

Journal of Biological Chemistry

137

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The human enzyme paraoxonase-2 (PON2) has two functions, an enzymatic lactonase activity and the reduction of intracellular oxidative stress. As a lactonase, it dominantly hydrolyzes bacterial signaling molecule 3OC12 and may contribute to the defense against pathogenic Pseudomonas aeruginosa. By its anti-oxidative effect, PON2 reduces cellular oxidative damage and influences redox signaling, which promotes cell survival. This may be appreciated but also deleterious given that high PON2 levels reduce atherosclerosis but may stabilize tumor cells. Here we addressed the unknown mechanisms and linkage of PON2 enzymatic and anti-oxidative function. We demonstrate that PON2 indirectly but specifically reduced superoxide release from the inner mitochondrial membrane, irrespective whether resulting from complex I or complex III of the electron transport chain. , was critical to its activity. Importantly, none of these mutations altered the anti-oxidative/anti-apoptotic function of PON2, demonstrating unrelated activities of the same protein. Collectively, our study provides detailed mechanistic insight into the functions of PON2, which is important for its role in innate immunity, atherosclerosis, and cancer.

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“…It has been argued that during monocytic maturation into macrophages, PON2 stimulation may modulate cellular superoxide anion production. 43 …”

Section: Disease Progression Genesmentioning

confidence: 99%

Gene expression signature of primary imatinib-resistant chronic myeloid leukemia patients

et al. 2006

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Paraoxonase 2 (PON2) expression is upregulated via a reduced-nicotinamide-adenine-dinucleotide-phosphate (NADPH)-oxidase-dependent mechanism during monocytes differentiation into macrophages

Cited by 61 publications

References 48 publications

Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated

Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated

One Enzyme, Two Functions

Gene expression signature of primary imatinib-resistant chronic myeloid leukemia patients

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