Paraoxonase 1 status is a major Janus-faced component of mild and moderate acute ischemic stroke and consequent disabilities

Increased malondialdehyde and nitric oxide formation, lowered total radical trapping capacity coupled with psychological stressors largely predict the phenome of first-episode mild depression in undergraduate students

“…In contrast to the a priori hypothesis, SDMD and FE-SDMD are not accompanied by increased AOPP and LOOH levels, and by lowered -SH groups and activities of the two catalytic sites of the PON1 enzyme (Brinholi et al 2023; Maes et al, 2011; Moreira et al, 2019). Another unexpected finding is that both SDMD and FE-SDMD were associated with decreased concentrations of LOOH.…”

“…PON1 status was determined by AREase and CMPAase activity and PON1 Q192R genotypes in the present investigation (Brinholi et al, 2023;Maes et al, 2022;Matsumoto et al, 2021;Moreira et al, 2017;. We investigated the rate of phenylacetate hydrolysis at low salt concentration by measuring the activity of AREase and CMPAase (Sigma, St. Louis, MO, USA) ( (Brinholi et al, 2023;Maes et al, 2022;Matsumoto et al, 2021;Moreira et al, 2017;. A Perkin Elmer® EnSpire model microplate reader (Waltham, MA, USA) was used to monitor the rate of phenylacetate hydrolysis at a constant temperature of 25 ˚C for 4 min (16 measurements with 15 s between each reading).…”

“…Moreira et al (2019) reported that, in mood disorders, ROI as measured by the number of previous depressive and manic episodes, is significantly correlated with decreased total PON1 and CMPAase activities. Therefore, it is difficult to draw comparisons between the findings of this study and those of the aforementioned research due to substantial differences in the study populations : Brinholi et al (2023) and Moreira et al (2019) examined recurrent MDD and bipolar depression, while we examined (FE-)SDMD. In addition, it is difficult to compare our findings with most prior publications on PON1 activities because most of those publications did not include both enzyme activities of PON1 or drew incorrect conclusions regarding its catalytic sites (Moreira et al, 2019).…”

“…All inter-assay coefficients of variation were <10.0%). PON1 status was determined by AREase and CMPAase activity and PON1 Q192R genotypes in the present investigation (Brinholi et al, 2023;Maes et al, 2022;Matsumoto et al, 2021;Moreira et al, 2017;. We investigated the rate of phenylacetate hydrolysis at low salt concentration by measuring the activity of AREase and CMPAase (Sigma, St. Louis, MO, USA) ( (Brinholi et al, 2023;Maes et al, 2022;Matsumoto et al, 2021;Moreira et al, 2017;.…”