Parametric optimization of electric field strength for cancer electrochemotherapy on a chip-based model

Zhao, Deyao; Wu, Mengxi; Dong, Hongjuan; Liang, Zicai; Wei, Zewen; Li, Zhihong

doi:10.7150/thno.21099

Cited by 9 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, animal models for drug testing are labor/time intensive, costly, and most importantly, often yield untranslatable results due to the physiological differences between humans and animal models 11-14. Therefore, the creation of cost-effective, reliable, and pragmatic in vitro models that can be used for accurately screening anticancer drug effects as well as overcoming the drawbacks of conventional models is of great importance for improving the current clinical management of primary tumor and metastatic cancer 15-18.…”

Section: Introductionmentioning

confidence: 99%

Metastasis-on-a-chip mimicking the progression of kidney cancer in the liver for predicting treatment efficacy

Wang¹,

Wu²,

Wu³

et al. 2020

Theranostics

View full text Add to dashboard Cite

Metastasis is one of the most important factors that lead to poor prognosis in cancer patients, and effective suppression of the growth of primary cancer cells in a metastatic site is paramount in averting cancer progression. However, there is a lack of biomimetic three-dimensional (3D) in vitro models that can closely mimic the continuous growth of metastatic cancer cells in an organ-specific extracellular microenvironment (ECM) for assessing effective therapeutic strategies.Methods: In this metastatic tumor progression model, kidney cancer cells (Caki-1) and hepatocytes (i.e., HepLL cells) were co-cultured at an increasing ratio from 1:9 to 9:1 in a decellularized liver matrix (DLM)/gelatin methacryloyl (GelMA)-based biomimetic liver microtissue in a microfluidic device.Results: Via this model, we successfully demonstrated a linear anti-cancer relationship between the concentration of anti-cancer drug 5-Fluorouracil (5-FU) and the percentage of Caki-1 cells in the co-culture system (R2 = 0.89). Furthermore, the Poly(lactide-co-glycolide) (PLGA)-poly(ethylene glycol) (PEG)-based delivery system showed superior efficacy to free 5-FU in killing Caki-1 cells.Conclusions: In this study, we present a novel 3D metastasis-on-a-chip model mimicking the progression of kidney cancer cells metastasized to the liver for predicting treatment efficacy. Taken together, our study proved that the tumor progression model based on metastasis-on-a-chip with organ-specific ECM would provide a valuable tool for rapidly assessing treatment regimens and developing new chemotherapeutic agents.

show abstract

Section: Introductionmentioning

confidence: 99%

Metastasis-on-a-chip mimicking the progression of kidney cancer in the liver for predicting treatment efficacy

Wang¹,

Wu²,

Wu³

et al. 2020

Theranostics

View full text Add to dashboard Cite

show abstract

“…In spite of the extensive clinical application of cisplatin, the side effects and resistance of patients towards this drug have promoted the development of novel combination therapy 11,12 . Amongst, electrochemotherapy 12–15 is a novel therapeutical strategy for the treatment of solid tumors by exposing the cells to electric fields to improve the bioactivity of non‐permeable or low permeable anticancer drugs, such as cisplatin. It is reported that the introduction of electric fields can effectively enhance the cytotoxicity of cisplatin via increasing the cell membrane permeability to this drug 16,17 .…”

Section: Introductionmentioning

confidence: 99%

Cisplatin under oriented external electric fields: A deeper insight into electrochemotherapy at the molecular level

Zhang

Xiao-ling

et al. 2020

Int J of Quantum Chemistry

View full text Add to dashboard Cite

Electrochemotherapy is an effective strategy for the treatment of solid tumors by exposing tumor cells to electric fields to enhance the bioactivity of non‐permeable or low permeable anticancer drugs, such as cisplatin. To understand the improved efficiency of cisplatin in the electrochemotherapy, the effects of oriented external electric fields (OEEFs) on the geometric structure and relevant electronic properties of cisplatin have been systemically investigated by density functional theory (DFT) computations in this work. Our results reveal that the presence of selective OEEFs on cisplatin can not only weaken its Pt‐Cl bonds, but also enhance the intramolecular charge transfer in it, which effectively accelerates the critical hydrolysis step involved in the mechanism of biological activity for this drug. Moreover, the chosen OEEFs can facilitate the attack of the singly aquated cis‐[Pt(NH3)2(H2O)Cl]+ on DNA, and enlarge the water solubility of cisplatin and its aquated product. These OEEF‐induced geometric and electronic modifications can indeed help to improve the antitumor activity of cisplatin, which provides a deeper insight into the higher efficacy of electrochemotherapy than traditional chemotherapy from a molecular point of view.

show abstract

“…Electrochemotherapy (ECT) [25][26][27] is an effective treatment strategy of solid tumors by exposing tumor issue under pulse electric fields to enhance the anticancer activity of specific drugs. Previous studies have revealed that electrochemotherapy can selectively kill tumor cells to avoid harming the surrounding normal tissue, [28] and has been approved to be used in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Cyclometalated gold(III)‐hydride under oriented external electric fields: a new strategy to modulate its reactivity?

Li,

Cheng,

Jiang

et al. 2023

Chemistry A European J

View full text Add to dashboard Cite

Selected gold complexes have been regarded as promising anti‐cancer agents because they can bind with protein targets containing thiol or selenol moieties, but their clinical applications were hindered by the unbiased binding towards off‐target thiol‐proteins. Recently, a novel gold(III)‐hydride complex (abbreviated as 1) with visible light‐induced thiol reactivity has been reported as potent photo‐activated anticancer agents (Angew. Chem. Int. Ed., 2020, 132, 11139). To explore new strategies to stimuli this potential antitumor drug, the effect of oriented external electric fields (OEEFs) on its geometric structure, electronic properties, and chemical reactivity was systematically investigated. Results reveal that imposing external electric fields along the Au−H bond of 1 can effectively activate this bond, which is conducive to its dissociation and the binding of Au site to potential targets. Hence, this study provides a new OEEF‐strategy to activate this reported gold(III)‐hydride, revealing its potential application in electrochemical therapy. We anticipate this work could promote the development of more electric field‐activated anticancer agents. However, further experimental research should be conducted to verify the conclusions obtained in this work.

show abstract

Parametric optimization of electric field strength for cancer electrochemotherapy on a chip-based model

Cited by 9 publications

References 39 publications

Metastasis-on-a-chip mimicking the progression of kidney cancer in the liver for predicting treatment efficacy

Metastasis-on-a-chip mimicking the progression of kidney cancer in the liver for predicting treatment efficacy

Cisplatin under oriented external electric fields: A deeper insight into electrochemotherapy at the molecular level

Cyclometalated gold(III)‐hydride under oriented external electric fields: a new strategy to modulate its reactivity?

Contact Info

Product

Resources

About